Poly(3-Hydroxybutyrate)-Multiwalled Carbon Nanotubes Electrospun Scaffolds Modified with Curcumin

Abstract

Appropriate selection of suitable materials and methods is essential for scaffolds fabrication in tissue engineering. The major challenge is to mimic the structure and functions of the extracellular matrix (ECM) of the native tissues. In this study, an optimized 3D structure containing poly(3-hydroxybutyrate) (P3HB), multiwalled carbon nanotubes (MCNTs) and curcumin (CUR) was created by electrospinning a novel biomimetic scaffold. CUR, a natural anti-inflammatory compound, has been selected as a bioactive component to increase the biocompatibility and reduce the potential inflammatory reaction of electrospun scaffolds. The presence of CUR in electrospun scaffolds was confirmed by 1H NMR and Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) revealed highly interconnected porosity of the obtained 3D structures. Addition of up to 20 wt% CUR has enhanced mechanical properties of the scaffolds. CUR has also promoted in vitro bioactivity and hydrolytic degradation of the electrospun nanofibers. The developed P3HB-MCNT composite scaffolds containing 20 wt% of CUR revealed excellent in vitro cytocompatibility using mesenchymal stem cells and in vivo biocompatibility in rat animal model study. Importantly, the reduced inflammatory reaction in the rat model after 8 weeks of implantation has also been observed for scaffolds modified with CUR. Overall, newly developed P3HB-MCNTs-CUR electrospun scaffolds have demonstrated their high potential for tissue engineering applications.

Novel strategy of cartilage repairing via application of P. atlantica with stem cells and collagen

“…Cell morphology revealed fibroblastic‐like adherent cells in all culture flasks. Flow cytometric findings of the current study was in line with the results of previous studies confirming the expression of CD44 (95.8%) and CD90 (77.2%) as mesenchymal stem cell markers and negative expression for CD34 and CD45 as hematopoietic cell markers with only 2.39% and 2.73% of cells, respectively 36 …”

Synthesis of Hydrophilic Poly(butylene succinate-butylene dilinoleate) (PBS-DLS) Copolymers Containing Poly(Ethylene Glycol) (PEG) of Variable Molecular Weights

“…Polymers, which are derived from renewable resources, are becoming increasingly more important as they are one of the key approaches in solving environmental problems such as increasing pollution and energy shortages caused by petroleum consumption [ 1 , 2 ]. Another great advantage is their use in medical applications, including tissue engineering in soft and hard tissue repair, drug delivery systems and resorbable sutures [ 3 , 4 , 5 , 6 ]. While the majority of research in recent decades has been focused on poly(lactic acid)/polylactide (PLA) and its copolymers, increasing interest is being directed towards another aliphatic polyester, poly(butylene succinate) (PBS), characterized by interesting physical and biological properties [ 7 ].…”

Review of Hybrid Materials Based on Polyhydroxyalkanoates for Tissue Engineering Applications

“…MWCNT addition resulted in a significant increase in the scaffold’s elastic Young’s modulus, tensile strength and yield strength [ 54 ]. It was found that incorporation of curcumin up to 20 wt% into PHB/MWCNT electrospun scaffolds had a significant effect on increasing ultimate strength values as compared to the neat PHB nanofibers [ 81 ].…”

“…It has been shown that addition of graphene nanoplatelets into PHB matrix led to decrease in the rate of degradation due to the non-degradable nature of graphene [ 89 ]. Incorporation of 20% curcumin increased the biodegradation rate of PHB/MWCNT electrospun nanofibers to about 35% of mass loss after 4 weeks compared to 6% mass loss of neat PHB nanofibers [ 81 ].…”

“…In another work, curcumin loaded PHB/MWCNT electrospun scaffolds forced mesenchymal stem cells to differentiate towards osteoblasts. In vivo biocompatibility studies revealed that curcumin strongly reduced inflammatory reaction after 8 weeks of implantation of the scaffolds [ 81 ].…”