Poly(3-hydroxybutyrate- co -3-hydroxyvalerate) production in a system with external cell recycle and limited nitrogen feeding during the production phase

Schmidt, Mélodi; Ienczak, Jaciane Lutz; Quines, Luci Kelin de Menezes; Zanfonato, Kellen; Schmidell, Willibaldo; Aragão, Gláucia Maria Falcão de

doi:10.1016/j.bej.2016.04.013

Cited by 34 publications

(25 citation statements)

References 26 publications

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“…Around 40-48% of the total production cost of PHA can be ascribed to raw materials (Schmidt et al, 2016). As raw material is the main carbon source for PHA synthesis and bacteria growing, using organic waste as a source of organic matter shows as a 7 promising alternative for the reduction in PHA production costs.…”

Section: Waste Carbon Sourcementioning

confidence: 99%

“…A mineral synthetic medium including alkylphenols or mono-aromatic hydrocarbons was used as synthetic industrial wastewaters (Venkateswar-Reddy et al, 2015). The production of PHA was evaluated by Schmidt et al (2016) using a glucose solution in order to simulate the carbon concentration usually found in agro-industrial wastes.…”

Section: Waste Carbon Sourcementioning

confidence: 99%

“…Two main reasons to not require any pre-treatment have been observed. On one hand, the authors used a synthetic waste as a carbon source (Schmidt et al, 2016;Cha et al, 2016;Palmeiro-Sánchez et al, 2016;Amulya et al, 2016;Chen et al, 2016a;Venkateswar-Reddy et al, 2016;Fradinho et al, 2016) or, on the other hand, the waste used was rich in sugars or VFA (Cruz et al, 2015;Cruz et al, 2016;Elain et al, 2016;de Paula et al, 2016). All pilot-scale studies showed a substrate pre-treatment (Table 3).…”

Section: Substrate Pre-treatmentmentioning

confidence: 99%

“…The genus Bacillus was the most employed in pure culture (Patel et al, 2015;Ribeiro et al, 2016;Pan et al, 2016;Rodriguez-Contreras et al, 2016;Venkateswar-Reddy et al, 2015). Some bacteria of the genus Cupriavidus, mainly C. necator, were also extensively employed for PHA production (Schmidt et al, 2016;Cruz et al, 2014;Cruz et al, 2015;Ribeiro et al, 2016;Haas et al, 2015). Genera Haloferax and Halomonas were also employed at lab-scale, but to a lesser extent than genus Bacillus and genus Cupriavidus (Alsafadi and Al-Mashaqbeh, 2017;Elain et al, 2016;Pais et al, 2016;Bera et al, 2015).…”

Section: Substrate Pre-treatmentmentioning

confidence: 99%

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Challenges of scaling-up PHA production from waste streams. A review

Rodríguez-Pérez

Serrano

Pantión

et al. 2018

Journal of Environmental Management

217

106

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The search for new materials that replace fossil fuel-based plastics has been focused on biopolymers with similar physicochemical properties to fossil fuel-based plastics, such as Polyhydroxyalkanoates (PHA). The present paper reviews the challenges of scaling-up PHA production from waste streams during the period from 2014 to 2016, focusing on the feasibility of the alternatives and the most promising alternatives to its scaling-up. The reviewed research studies mainly focus on reducing costs or obtaining more valuable polymers. In the future, the integration of PHA production into processes such as wastewater treatment plants, hydrogen production or biodiesel factories could enhance its implementation at industrial scale.

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Section: Waste Carbon Sourcementioning

confidence: 99%

Section: Waste Carbon Sourcementioning

confidence: 99%

Section: Substrate Pre-treatmentmentioning

confidence: 99%

Section: Substrate Pre-treatmentmentioning

confidence: 99%

See 2 more Smart Citations

Challenges of scaling-up PHA production from waste streams. A review

Rodríguez-Pérez

Serrano

Pantión

et al. 2018

Journal of Environmental Management

217

106

View full text Add to dashboard Cite

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“…The Gram-negative, non-spore forming soil bacterium, Cupriavidus necator H16, is of biotechnological importance principally due to its ability to accumulate >80% of its dry cell weight as polyhydroxyalkanoate (PHA) -a biodegradable polymer and an alternative to petroleum-based polymers (1,2). The accumulation of PHA (specifically 3polyhydroxybutyrate (3-PHB)) by the bacterium is a carbon conservation mechanism.…”

Section: Introductionmentioning

confidence: 99%

Data Driven Modelling of a Chemically Defined Growth Medium for Cupriavidus necator H16

Azubuike

Edwards

Gatehouse

et al. 2019

Preprint

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Cupriavidus necator is a Gram-negative soil bacterium of major biotechnological interest. It is a producer of the bioplastic 3-polyhydroxybutyrate, has been exploited in bioremediation processes, and it's lithoautotrophic capabilities suggest it may function as a microbial cell factory upgrading renewable resources to fuels and chemicals. It remains necessary however to develop appropriate experimental resources to permit controlled bioengineering and system optimisation of this microbial chassis. A key resource for physiological, biochemical and metabolic studies of any microorganism is a chemically defined growth medium. Here we use 1 mL micro-well cell cultures, automated liquid handling and a statistical engineering approach to develop a model that describes the effect of key media components and their interactions on C. necator culture cell density. The model is predictive and was experimentally validated against novel media compositions. Moreover, the model was further validated against larger culture volumes at 100 mL and 1 L volumes and found to correlate well. This approach provides valuable and quantifiable insights into the impact of media components on cell growth as well as providing predictions to guide culture scale-up.

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Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

Luo

Liu

et al. 2019

Biotechnology Journal

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In recent years, naturally biodegradable polyhydroxyalkanoate (PHA) monopolymers have become focus of public attentions due to their good biocompatibility. However, due to its poor mechanical properties, high production costs, and limited functionality, its applications in materials, energy, and biomedical applications are greatly limited. In recent years, researchers have found that PHA copolymers have better thermal properties, mechanical processability, and physicochemical properties relative to their homopolymers. This review summarizes the synthesis of PHA copolymers by the latest biosynthetic and chemical modification methods. The modified PHA copolymer could greatly reduce the production cost with elevated mechanical or physicochemical properties, which can further meet the practical needs of various fields. This review further summarizes the broad applications of modified PHA copolymers in biomedical applications, which might shred lights on their commercial applications.

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Poly(3-hydroxybutyrate- co -3-hydroxyvalerate) production in a system with external cell recycle and limited nitrogen feeding during the production phase

Cited by 34 publications

References 26 publications

Challenges of scaling-up PHA production from waste streams. A review

Challenges of scaling-up PHA production from waste streams. A review

Data Driven Modelling of a Chemically Defined Growth Medium for Cupriavidus necator H16

Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

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