Poly (3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) scaffolds coated with PhaP‐RGD fusion protein promotes the proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells in vitro

Abstract

Human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs) have been widely used in tissue engineering. The aim of this study is to evaluate the ability of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds coated with polyhydroxyalkanoate binding protein fused with arginyl-glycyl-aspartic acid (PhaP-RGD) to promote the proliferation and chondrogenic differentiation of hUC-MSCs seeded on them. The PhaP-RGD fusion protein was expressed by Escherichia coli. PHBHHx films were coated with PhaP-RGD fusion protein and the physiochemical properties were examined. hUC-MSCs were seeded on PHBHHx films with or without PhaP-RGD precoating and tested for changes in morphology, viability, and chondrogenic differentiation. We found that PhaP-RGD-coated PHBHHx films had similar surface morphology to uncoated PHBHHx. The water contact angle of the coated PHBHHx surface was lower than that of the uncoated surface (10.63° vs. 98.69°). At 7 and 14 days after seeding, the PhaP-RGD-coated PHBHHx group showed greater numbers of viable cells compared to the uncoated PHBHHx group. The expression levels of aggrecan and collagen II were enhanced in the PhaP-RGD-coated PHBHHx group relative to the uncoated PHBHHx group. Histological analysis using toluidine blue staining showed elevated formation of proteoglycan producing chondrocytes in the PhaP-RGD-coated PHBHHx group. Additionally, the synthesis of proteoglycan and collagen was significantly enhanced within the PhaP-RGD constructs. Taken together, PhaP-RGD coating promotes the proliferation and chondrogenic differentiation of hUC-MSCs seeded on PHBHHx films. PhaP-RGD-coated PHBHHx may be a useful scaffold for cartilage tissue engineering.

Human umbilical cord derivatives regenerate intervertebral disc

“…The alternative studies led to isolation of MSCs from perinatal sources, which are more primitive, potent and proliferative, and can be acceptable for allogeneic and xenogeneic transplantation studies (Beeravolu et al ., ; Rachakatla et al ., ; Weiss and Troyer, ). Consequently, several reports have indicated that perinatal MSCs have the potential to produce ECM which is an integral component of NP of the IVD (Anderson et al ., ; Chon et al ., ; Ibrahim et al ., ; Li et al ., , ).…”

Chondrogenic commitment of human umbilical cord blood-derived mesenchymal stem cells in collagen matrices for cartilage engineering

“…The use of matrix scaffolds is a key component in the success of cartilage engineering. A number of recent studies have shown that UCB-MSCs are able to differentiate into chondrocytes in scaffolds454647. For example, equine UCB-MSCs in membrane culture at low oxygen tension in the presence of TGF-β3 (10 ng/ml) produce a comparatively larger amount of high-quality neo-cartilage in comparison with differentiated cells in the pellet model46.…”

The Effect of miR122 and miR185 on Hepatic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells

“…The pluripotent stem cells surface markers express in hUC-MSCs including Oct-4, Sox-2 [36] . hUC-MSCs have the potential of multidirectional differentiation, and which can differentiate into chondrocytes [37] , hepatocytes [38] , and adipocytes [39] . The cells we obtained from umbilical cord express CD90 (99.16% ± 0.85%) and CD105 (99.26% ± 0.78%), but no expression of CD34 (0.59% ± 0.16%) and CD45 (1.50% ± 2.04%) by these cells and 66.57% ± 4.61% cells expressed positively Oct-4 analyzed by ow cytometry.…”