Poly(3-hydroxyalkanoate) produced from Pseudomonas aeruginosa 42A2 (NCBIM 40045): Effect of fatty acid nature as nutrient

Study of the crosslinking reaction (natural and UV induced) in polyunsaturated PHA from linseed oil

“…As previously reported, P. aeruginosa 42A2 is a versatile bacterium that produces mcl-PHA random copolymers when grown on either residual agro-industrial hydrophobic substrates [13] or defined alkyl substrates [14].…”

Effect of emulsified feeding of oily substrate via submerged ceramic membranes on surfactant production in Pseudomonas aeruginosa fermentation

“…The duration of the conventional batch process for PHA production was 48 h, during which Fig. 5 Droplet-size distribution achieved with feeding via 5 lm membrane at different feeding modes; distribution achieved by mixing-only of the solution at 100 rpm is shown for comparison purpose 30 g/L of oil (fed in the beginning after inoculation) was consumed and roughly 22 g/L biomass (dcw) was produced along with approximately 5 g/L of the product [21]. Total reaction volume was 2 L. A high amount of foam production was a major problem faced with this process which not only affected sterile working but also the accurate analysis of the process, due to significant loss of culture volume that left the reactor along with exiting foam.…”

“…Thus, a lot of substrate was not converted to the desired product. Nevertheless, it could be observed that with emulsified feeding a relative excess of the carbon source (oil) in the culture could be obtained, which is important for PHA production [21]. In the same process time, a higher amount of substrate could be consumed by the cells resulting in a higher production and productivity, which could further be improved by engineering the microorganism to produce less EPS and more of the desired product.…”