Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures

Rienzi, Laura; Martínez, Francisco Martínez; Ubaldi, Filippo Maria; Minasi, MG; Iacobelli, Marcello; Tesařík, Jan; Greco, Ermanno

doi:10.1093/humrep/deh101

Cited by 191 publications

(104 citation statements)

References 30 publications

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“…Since there are considerable apparent between-species and study differences, it was appropriate to examine the effect of vitrifying MII equine oocytes with or without their cumulus investment on post-warming meiotic spindle quality. And while post-warming spindle quality was generally poor in vitrified-warmed oocytes, despite a 6 h post-warming incubation to allow spindle reassembly and repair (Rienzi et al 2004), cumulus-enclosed oocytes fared significantly better than denuded oocytes (38 vs 3% normal spindles). In short, our results support the hypothesis that the cumulus helps to protect equine oocytes from damage during vitrification.…”

Section: Discussionmentioning

confidence: 98%

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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Vitrifying oocytes is a potentially valuable means of preserving the female germ line, but significantly compromises oocyte developmental competence. This study examined the hypothesis that the cumulus complex protects the oocyte during vitrification. Vitrified-warmed immature cumulus oocyte complexes (COCs) were labelled with a plasma membrane impermeant DNA marker (ethidium homodimer-1) to examine the percentage and location of dead cumulus cells, and to investigate the effect of the proportion of dead cells (C1,C2 or C3) on the success of in vitro maturation (IVM). Further, oocytes were labelled for connexin-43 or injected with Lucifer yellow dye to determine whether the integrity of the gap junctions between an oocyte and its cumulus was compromised by vitrification. Finally, the effect of denuding immature and mature oocytes on their ability to withstand vitrification was examined. Cryopreserving immature COCs increased the number of dead cumulus cells (13 vs 2.6% for controls; P!0.05). However, an increased proportion of dead cumulus cells did not affect post-warming maturation rates (w30% MII ) presumably because dead cells were located at the periphery of the cumulus mass and cumulus-oocyte gap junction communication was not disrupted. Moreover, cumulus removal prior to IVM or vitrification indicated that while the cumulus does protect immature oocytes during vitrification it does so by mechanisms other than support during maturation. Cumulus presence was also found to protect mature equine oocytes against vitrification-induced damage since cumulus-enclosed MII oocytes preserved their meiotic spindle quality better during vitrification than denuded oocytes (38.1 vs 3.1% normal spindles; P!0.05).

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Section: Discussionmentioning

confidence: 98%

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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“…In addition, although depolymerization of the oocyte's meiotic spindle is known to occur, a number of studies have now shown that the spindle reforms in the majority of oocytes after thawing [24][25][26][27][28][29]. The advent of ICSI for insemination has overcome any concern regarding potential premature cortical granule reaction [11].…”

Section: Introductionmentioning

confidence: 99%

Oocyte cryopreservation: is it time to remove its experimental label?

Noyes

Boldt

Nagy

2010

J Assist Reprod Genet

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As more reproductive-age women survive cancer at the expense of gonadotoxic therapy, the need for viable fertility preservation options has become paramount. Embryo cryopreservation, often using donor sperm, has been the standard offered these women over the past 20 years. Preservation of unfertilized oocytes now represents an acceptable and often equally viable alternative, particularly for single women, due to technologic advances made in the past decade. Given such, oocyte cryopreservation's experimental designation and need for IRB approval should thus be revisited.

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“…Disruption of the meiotic spindle increases the risk of aneuploidy. These concerns were studied by Rienzi et al by slow freezing oocytes and looking at the meiotic spindle using computer-assisted polarization microscopy (Rienzi et al, 2004). This technique allowed visualization of the spindle in real time by evaluating living oocytes.…”

Section: Is Oocyte Cryopreservation "Experimental?"mentioning

confidence: 99%

Oocyte Cryopreservation for the Elective Preservation of Reproductive Potential

Bigelow¹,

Copperman²

2012

Current Frontiers in Cryobiology

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Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures

Abstract: The current techniques of oocyte freezing and thawing inevitably cause meiotic spindle destruction. All spindles observed in thawed oocytes result from post-thaw reconstruction.

Cited by 191 publications

References 30 publications

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Oocyte cryopreservation: is it time to remove its experimental label?

Oocyte Cryopreservation for the Elective Preservation of Reproductive Potential

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