POLR3G promotes EMT via PI3K/AKT signaling pathway in bladder cancer

Abstract

RNA Polymerase III Subunit G (POLR3G) promotes tumorigenesis, metastasis, cancer stemness, and chemoresistance of breast cancer and lung cancer; however, its biological function in bladder cancer (BLCA) remains unclear. Through bioinformatic analyses, we found that POLR3G expression was significantly elevated in BLCA tumor tissues and was associated with decreased survival. Multivariate Cox analysis indicated that POLR3G could serve as an independent prognostic risk factor. Our functional investigations revealed that POLR3G deficiency resulted in reduced migration and invasion of BLCA cells both in vitro and in vivo. Additionally, the expressions of EMT‐related mesenchymal markers were also downregulated in POLR3G knockdown cells. Mechanistically, we showed that POLR3G could activate the PI3K/AKT signaling pathway. Inhibition of this pathway with LY294002 reduced the enhanced migration and invasion of BLCA cells induced by POLR3G overexpression, whereas the activation of this pathway using 740Y‐P restored the abilities that were inhibited by POLR3G knockdown. Taken together, our findings suggested that POLR3G is a prognostic predictor for BLCA and promotes EMT of BLCA through activation of the PI3K/AKT signaling pathway.

SLC7A11 promotes EMT and metastasis in invasive pituitary neuroendocrine tumors by activating the PI3K/AKT signaling pathway

“…In glioblastoma, NQO1 could facilitate EMT through the PI3K/Akt/mTOR pathway ( 39 ). Chen also reported POLR3G promotes EMT of BLCA through regulation of the PI3K/AKT pathway ( 40 ). KEGG analysis indicated an enrichment of DEGs in the PI3K/AKT signaling pathway.…”

Association between lung cancer and bladder cancer risk: a bidirectional Mendelian randomization study

“…Many studies have found that the development of lung cancer and bladder cancer was associated with activation of the PI3K/Akt/mTOR and MAPK signaling pathway [30][31][32][33]. Chen et al discovered that RNA Polymerase III Subunit G (POLR3G) plays a contributory role in the development of lung cancer and bladder cancer [34]. Lung cancer and bladder cancer may share similar biological mechanisms, which could explain the causal relationship between these two cancers.…”