Poloxamer/sodium cholate co-formulation for micellar encapsulation of doxorubicin with high efficiency for intracellular delivery: An in-vitro bioavailability study

Tasca, Elisamaria; Andreozzi, Patrizia; Giudice, Alessandra Del; Galantini, Luciano; Schillén, Karin; Giuliani, Anna Maria; Ramírez, María de los Ángeles; Moya, Sergio; Giustini, Mauro

doi:10.1016/j.jcis.2020.06.096

Cited by 22 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Investigations on the interaction between BAs and poloxamers have recently demonstrated that BAs can be adsorbed on the corona of block copolymer micelles, thereby promoting block copolymers as alternative BA sequestrants [135][136][137]. Similar Pluronic/BA mixed micelles were proven to be appealing drug-delivery vehicles and to efficiently load drugs like Clozapine [138] and Doxorubicin [139,140]. Because the BAs are anionic surfactants, it is expected that cationic block copolymers provide better BA binding and sequestration.…”

Section: Ba Polymer Mixturesmentioning

confidence: 99%

Physiology and Physical Chemistry of Bile Acids

Gregorio

Cautela

Galantini

2021

IJMS

Self Cite

View full text Add to dashboard Cite

Bile acids (BAs) are facial amphiphiles synthesized in the body of all vertebrates. They undergo the enterohepatic circulation: they are produced in the liver, stored in the gallbladder, released in the intestine, taken into the bloodstream and lastly re-absorbed in the liver. During this pathway, BAs are modified in their molecular structure by the action of enzymes and bacteria. Such transformations allow them to acquire the chemical–physical properties needed for fulling several activities including metabolic regulation, antimicrobial functions and solubilization of lipids in digestion. The versatility of BAs in the physiological functions has inspired their use in many bio-applications, making them important tools for active molecule delivery, metabolic disease treatments and emulsification processes in food and drug industries. Moreover, moving over the borders of the biological field, BAs have been largely investigated as building blocks for the construction of supramolecular aggregates having peculiar structural, mechanical, chemical and optical properties. The review starts with a biological analysis of the BAs functions before progressively switching to a general overview of BAs in pharmacology and medicine applications. Lastly the focus moves to the BAs use in material science.

show abstract

Section: Ba Polymer Mixturesmentioning

confidence: 99%

Physiology and Physical Chemistry of Bile Acids

Gregorio

Cautela

Galantini

2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…This sensitivity of the fluorescent lifetime to the local micro-environment can be taken advantage of to track the liberation of a fluorescent drug from a polymer NP, for example. If we look at the case of DOX, a widely used chemotherapeutic drug that happens to be intrinsically fluorescent, we can measure its fluorescent lifetime to determine whether or not it has been liberated from its delivery vessel [ 86 , 87 , 88 , 89 , 90 ]. In a recent study, it was demonstrated that the encapsulation of DOX inside PEO–PPO–PEO triblock copolymers, which self-assemble into micelles above the critical concentration, was possible.…”

Section: In Vitro Techniquesmentioning

confidence: 99%

“…In a recent study, it was demonstrated that the encapsulation of DOX inside PEO–PPO–PEO triblock copolymers, which self-assemble into micelles above the critical concentration, was possible. The encapsulation acts as a means to keep the DOX in an apolar environment, and as such we can distinguish it from free DOX via the different fluorescent lifetime, i.e., apolar vs. polar local micro-environment [ 90 ]. The lifetime value also distinguishes the location of DOX located inside the polar interior of the micelles or in the PEO corona.…”

Section: In Vitro Techniquesmentioning

confidence: 99%

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

et al. 2021

View full text Add to dashboard Cite

Nanoformulations offer multiple advantages over conventional drug delivery, enhancing solubility, biocompatibility, and bioavailability of drugs. Nanocarriers can be engineered with targeting ligands for reaching specific tissue or cells, thus reducing the side effects of payloads. Following systemic delivery, nanocarriers must deliver encapsulated drugs, usually through nanocarrier degradation. A premature degradation, or the loss of the nanocarrier coating, may prevent the drug’s delivery to the targeted tissue. Despite their importance, stability and degradation of nanocarriers in biological environments are largely not studied in the literature. Here we review techniques for tracing the fate of nanocarriers, focusing on nanocarrier degradation and drug release both intracellularly and in vivo. Intracellularly, we will discuss different fluorescence techniques: confocal laser scanning microscopy, fluorescence correlation spectroscopy, lifetime imaging, flow cytometry, etc. We also consider confocal Raman microscopy as a label-free technique to trace colocalization of nanocarriers and drugs. In vivo we will consider fluorescence and nuclear imaging for tracing nanocarriers. Positron emission tomography and single-photon emission computed tomography are used for a quantitative assessment of nanocarrier and payload biodistribution. Strategies for dual radiolabelling of the nanocarriers and the payload for tracing carrier degradation, as well as the efficacy of the payload delivery in vivo, are also discussed.

show abstract

“…In this framework, fluorescence microscopy and laser scanning confocal microscopy (LSCM) are key experimental techniques to unravel the behavior of nanomaterials designed for pharmaceutical applications in biological environments [10][11][12][13]. Compared to other imaging techniques, in fluorescence microscopy the signal (and, consequently, the contrast) is provided by fluorescence (or autofluorescence); therefore, choosing the appropriate fluorescent probes allows for the highlighting of specific characteristics of the sample (as hydrophobic/hydrophilic regions), and/or the labeling of selected groups of the sample of interest, in an extremely tunable and variable manner.…”

Section: Introductionmentioning

confidence: 99%

Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

et al. 2021

View full text Add to dashboard Cite

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

show abstract

Poloxamer/sodium cholate co-formulation for micellar encapsulation of doxorubicin with high efficiency for intracellular delivery: An in-vitro bioavailability study

Cited by 22 publications

References 43 publications

Physiology and Physical Chemistry of Bile Acids

Physiology and Physical Chemistry of Bile Acids

Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate

Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Contact Info

Product

Resources

About