Poloxamer bioadhesive hydrogel for buccal drug delivery: Cytotoxicity and trans-epithelial permeability evaluations using TR146 human buccal epithelial cell line

Zeng, Ni; Mignet, Nathalie; Dumortier, G.; Olivier, Elodie; Séguin, Johanne; Maury, Marc; Scherman, Daniel; Rat, Patrice; Boudy, Vincent

doi:10.1016/j.ijpharm.2015.09.045

Cited by 27 publications

(12 citation statements)

References 46 publications

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“…For MTT assay, the fibres were sterilized by UV radiation for 4 h first, then extracted in culture medium for 24 h and diluted into varying concentrations (10, 5, 2, 1 and 0.5 mg/ml) . TR146 cells were seeded in 96‐well plate at a density of about 1 × 10 4 cells per well.…”

Section: Methodsmentioning

confidence: 99%

Self-assembled liposome from core-sheath chitosan-based fibres for buccal delivery of carvedilol: formulation, characterization and in vitro and ex vivo buccal absorption

Chen

Pan

Duan

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives A novel drug delivery system based on self-assembled liposome from core-sheath nanofibres for buccal delivery of Carvedilol (Car) was explored. Methods The Car-loaded PVP/PC (phospholipids) layer was coated with chitosan-PVA (CS-PVA) or CS-PVP to increase retention period in the mouth. SEM, confocal laser scanning microscopy (CLSM), XRD and Fourier transform infrared spectroscopy were applied to characterize fibre diameter and drug state. Appearance, particle size and encapsulation efficiency of self-assembled liposome were investigated by transmission electron microscopy (TEM) and Zeta-sizer Nano. The dissolution test and permeation tests across porcine buccal mucosa and TR146 cell model also were run. Key findings Confocal laser scanning microscopy and XRD confirmed the coresheath structure of coaxial fibre and non-crystalline form of Car, separately. TEM demonstrated the sphere morphology of self-assembled liposome from spun fibres after contacting water. The dissolution test implied the ratio of PC to Car had a huge impact on drug release. The permeation tests across porcine buccal mucosa and TR146 cell model showed similar result, namely our formulation having a better permeation performance than Car suspension. The indirect toxicity against TR146 cells presented 5 mg/ml (or lower) of fibre extraction was safe for cells. Conclusions These researches exhibited this drug delivery system was promising and advantageous for Car buccal delivery.Core-sheath fiber for buccal permeation Jianting Chen et al.

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Section: Methodsmentioning

confidence: 99%

Self-assembled liposome from core-sheath chitosan-based fibres for buccal delivery of carvedilol: formulation, characterization and in vitro and ex vivo buccal absorption

Chen

Pan

Duan

et al. 2019

Journal of Pharmacy and Pharmacology

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“…TR146 cells were recently used for the evaluation of nanosystems 76,77 and for cytotoxicity studies of bioadhesive hydrogels for buccal drug delivery. 78 Approaches that have shown promising effects in enhancing transport of macromolecules in several in vitro and in vivo buccal model systems have been extensively reviewed previously. [79][80][81] TR146 cells grown at the air-liquid interface have also been used in commercially available 3D models of human oral mucosa.…”

Section: лmentioning

confidence: 99%

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

et al. 2018

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Understanding the function of oral mucosal epithelial barriers is essential for a plethora of research fields such as tumor biology, inflammation and infection diseases, microbiomics, pharmacology, drug delivery, dental and biomarker research. The barrier properties are comprised by a physical, a transport and a metabolic barrier, and all these barrier components play pivotal roles in the communication between saliva and blood. The sum of all epithelia of the oral cavity and salivary glands is defined as the blood-saliva barrier. The functionality of the barrier is regulated by its microenvironment and often altered during diseases. A huge array of cell culture models have been developed to mimic specific parts of the blood-saliva barrier, but no ultimate standard in vitro models have been established. This review provides a comprehensive overview about developed in vitro models of oral mucosal barriers, their applications, various cultivation protocols and corresponding barrier properties.

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“…MTT was utilized to assess the tissue viability of EpiOral TM after contact with the optimized wafers and films. The assay investigates the reduction of yellow MTT to an insoluble purple formazan predominantly by enzymes (succinate dehydrogenase) found in the mitochondria of viable cells [52,53,54]. The MTT assay demonstrated some level of cytotoxicity by NIC, with the non-viable cell’s inability to reduce yellow MTT to purple formazan.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Clay-Functionalized Wafers and Films for Nicotine Replacement Therapy via Buccal Mucosa

Boateng

Okeke

2019

Pharmaceutics

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The functional physicochemical properties of nicotine (NIC)-loaded composite freeze-dried wafers and solvent-evaporated films comprising hydroxypropylmethylcellulose (HPMC) and sodium alginate (SA), stabilized with magnesium aluminium silicate (MAS), have been reported. The formulations were characterized for swelling capacity, mucoadhesion, in vitro drug dissolution properties in simulated saliva (SS) and PBS at pH 6.8, and ex vivo and in vitro permeation using pig buccal mucosa membrane and EpiOralTM buccal tissue culture, respectively; finally, the cell viability of the EpiOralTM tissues after contact with the NIC-loaded formulations was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the functional characteristics compared with those of commercially available NIC strips. Swelling and NIC release from the HPMC–SA wafers were more prolonged (30 min) compared to the commercially available NIC strips which disintegrated rapidly and released the drug within 5 min. Generally, swelling, mucoadhesion, and drug release was faster in PBS than in SS, and the presence of MAS was essential for maintaining a high dose recovery compared to non-MAS formulations and commercial NIC strips, which showed lower percentage of NIC content, possibly due to evaporation during analysis. Permeation studies showed that the NIC released was able to cross both porcine buccal membrane and the EpiOralTM buccal tissue, with the latter showing higher permeation flux for all the formulations tested. All the NIC-loaded, MAS-stabilized formulations showed high tissue viability, with values above 80%, showing their great potential for use as buccal delivery platforms for NIC replacement therapy to aid smoking cessation.

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Poloxamer bioadhesive hydrogel for buccal drug delivery: Cytotoxicity and trans-epithelial permeability evaluations using TR146 human buccal epithelial cell line

Cited by 27 publications

References 46 publications

Self-assembled liposome from core-sheath chitosan-based fibres for buccal delivery of carvedilol: formulation, characterization and in vitro and ex vivo buccal absorption

Self-assembled liposome from core-sheath chitosan-based fibres for buccal delivery of carvedilol: formulation, characterization and in vitro and ex vivo buccal absorption

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

Evaluation of Clay-Functionalized Wafers and Films for Nicotine Replacement Therapy via Buccal Mucosa

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