Poloxamer 188 Exerts Direct Protective Effects on Mouse Brain Microvascular Endothelial Cells in an In Vitro Traumatic Brain Injury Model

Lotze, Felicia P.; Riess, Matthias L.

doi:10.3390/biomedicines9081043

Cited by 7 publications

(11 citation statements)

References 72 publications

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“…This includes the lowest cell viability and the worst membrane leakage, indicated by LDH release and FM1-43 incorporation, which makes reperfusion the critical turning point to intervene pharmacologically. Similar to a previous study in mouse brain microvascular ECs [36], our data indicate that P188 treatment upon reperfusion produced a dose-dependent attenuation of membrane leakage and cell death in mouse coronary artery ECs.…”

Section: Protection By P188supporting

confidence: 90%

Newly Developed Di-Block Copolymer-Based Cell Membrane Stabilizers Protect Mouse Coronary Artery Endothelial Cells against Hypoxia/Reoxygenation Injury

Gupta

Barajas

et al. 2023

Cells

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Reperfusion after ischemia causes additional cellular damage, known as reperfusion injury, for which there is still no effective remedy. Poloxamer (P)188, a tri-block copolymer-based cell membrane stabilizer (CCMS), has been shown to provide protection against hypoxia/reoxygenation (HR) injury in various models by reducing membrane leakage and apoptosis and improving mitochondrial function. Interestingly, substituting one of its hydrophilic poly-ethylene oxide (PEO) blocks with a (t)ert-butyl terminus added to the hydrophobic poly-propylene oxide (PPO) block yields a di-block compound (PEO-PPOt) that interacts better with the cell membrane lipid bi-layer and exhibits greater cellular protection than the gold standard tri-block P188 (PEO75-PPO30-PEO75). For this study, we custom-made three different new di-blocks (PEO113-PPO10t, PEO226-PPO18t and PEO113-PPO20t) to systemically examine the effects of the length of each polymer block on cellular protection in comparison to P188. Cellular protection was assessed by cell viability, lactate dehydrogenase release, and uptake of FM1-43 in mouse artery endothelial cells (ECs) following HR injury. We found that di-block CCMS were able to provide the same or better EC protection than P188. Our study provides the first direct evidence that custom-made di-block CCMS can be superior to P188 in improving EC membrane protection, raising their potential in treating cardiac reperfusion injury.

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Section: Protection By P188supporting

confidence: 90%

Newly Developed Di-Block Copolymer-Based Cell Membrane Stabilizers Protect Mouse Coronary Artery Endothelial Cells against Hypoxia/Reoxygenation Injury

Gupta

Barajas

et al. 2023

Cells

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“…Theoretically, the expression of CD31 in the NCHA group was expected to surpass that of the Gel group, yet experimental results revealed the contrary. Through a comprehensive review of the literature, it was found that high-molecular weight hyaluronic acid might hinder vascular formation [ 45 , 46 ], whereas F68 demonstrated a protective effect on damaged microvascular endothelial cells [ 47 ], which could elucidate this phenomenon.…”

Section: Resultsmentioning

confidence: 99%

Amorphous curcumin-based hydrogels to reduce the incidence of post-surgical intrauterine adhesions

Zhang,

He,

Chu

et al. 2024

Regenerative Biomaterials

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The incidence of intrauterine adhesions (IUA) was raised accompanied by the increasing utilization of intrauterine surgery. The postoperative physical barrier methods commonly used, such as balloons and other fillers, have limited effectiveness and may even cause further damage to the remaining endometrial tissue. Herein, we developed an injectable thermosensitive hydrogel using Pluronic F127/F68 as pharmaceutical excipients and curcumin as a natural active molecule. The hydrogel effectively addresses solubility and low bioavailability issues associated with curcumin. In vitro, drug release assays revealed that the amorphous curcumin hydrogel promotes dissolution and sustained release of curcumin. In vitro experiments reveal high biocompatibility of the hydrogel and its ability to enhance vascular formation while inhibiting the expression of fibrotic factor TGF-β1. To assess the effectiveness of preventing intrauterine adhesions, in vivo experiments were conducted using IUA rats and compared with a class III medical device, a new-crosslinked hyaluronic acid gel (NCHA). According to the study, curcumin hydrogel is more effective than the NCHA group in improving the regeneration of the endometrium, increasing the blood supply to the endometrium, and reducing the abnormal deposition of fibrin, thus preventing intrauterine adhesion more effectively. This study provides a promising strategy for treating and preventing IUA.

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“…Endothelial cells are integral to the function of the blood-brain barrier, and their detachment and disruption alters the function of the tight junctions in the blood-brain barrier which increases the permeability of injurious substances. Lotze et al investigated the effect of P188 on mouse brain microvascular endothelial cells in a combined model of simulated IR and compression (TBI) [ 16 , 56 ]. Mild injury models underwent 1 h of compression at the start of a 5-h hypoxia/2-h reoxygenation exposure.…”

Section: Astrocyte Endothelial Cell and Neuronal In Vitro Models Of T...mentioning

confidence: 99%

P188 Therapy in In Vitro Models of Traumatic Brain Injury

Zargari

Meyer

Riess

et al. 2023

IJMS

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Traumatic brain injury (TBI) is a significant cause of morbidity and mortality worldwide. Varied mechanisms of injury contribute to the heterogeneity of this patient population as demonstrated by the multiple published grading scales and diverse required criteria leading to diagnoses from mild to severe. TBI pathophysiology is classically separated into a primary injury that is characterized by local tissue destruction as a result of the initial blow, followed by a secondary phase of injury constituted by a score of incompletely understood cellular processes including reperfusion injury, disruption to the blood-brain barrier, excitotoxicity, and metabolic dysregulation. There are currently no effective pharmacological treatments in the wide-spread use for TBI, in large part due to challenges associated with the development of clinically representative in vitro and in vivo models. Poloxamer 188 (P188), a Food and Drug Administration-approved amphiphilic triblock copolymer embeds itself into the plasma membrane of damaged cells. P188 has been shown to have neuroprotective properties on various cell types. The objective of this review is to provide a summary of the current literature on in vitro models of TBI treated with P188.

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Poloxamer 188 Exerts Direct Protective Effects on Mouse Brain Microvascular Endothelial Cells in an In Vitro Traumatic Brain Injury Model

Cited by 7 publications

References 72 publications

Newly Developed Di-Block Copolymer-Based Cell Membrane Stabilizers Protect Mouse Coronary Artery Endothelial Cells against Hypoxia/Reoxygenation Injury

Newly Developed Di-Block Copolymer-Based Cell Membrane Stabilizers Protect Mouse Coronary Artery Endothelial Cells against Hypoxia/Reoxygenation Injury

Amorphous curcumin-based hydrogels to reduce the incidence of post-surgical intrauterine adhesions

P188 Therapy in In Vitro Models of Traumatic Brain Injury

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