Pollution-induced community tolerance of freshwater biofilms: measuring heterotrophic tolerance to Pb using an enzymatic toxicity test

Fechner, Lise C.; Dufour, Marine; Gourlay-Francé, Catherine

doi:10.1007/s10646-012-0964-6

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…A decrease of this parameter had previously been observed as tolerance levels increased for biofilms exposed to metals either in the laboratory or in the field (e.g. Fechner et al, 2012aFechner et al, , 2012b. However no such clear tendency was observed here over the four season experiment.…”

Section: Seasonal Variations Of Tolerance Measurementssupporting

confidence: 47%

“…The presence of those hereby unmonitored micropollutants (both organic and metal) during biofilm growth could be one of the explanation of biofilms' sensitivity to the low metal pressure monitored even at both downstream sites. As a matter of fact, metal exposure levels for each metal at both downstream sites are between 3 and 50 times smaller than exposure levels used to generate PICT effects in our previous microcosm experiments (Fechner et al, 2012a(Fechner et al, , 2011.…”

Section: 2mentioning

confidence: 77%

“…Studies performed in the laboratory under controlled conditions do show tolerance induction after exposure to a given toxicant. For instance, using the same short-term toxicity test, we previously observed an increase of tolerance to Cd, Ni, Zn and Pb for biofilms exposed separately to those metals in microcosms (Fechner et al, 2012a(Fechner et al, , 2011. However those experiments have also confirmed the existence of co-tolerance phenomenons.…”

Section: 2mentioning

confidence: 80%

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Impact of an urban multi-metal contamination gradient: Metal bioaccumulation and tolerance of river biofilms collected in different seasons

Faburé

Dufour²,

Autret

et al. 2015

Aquatic Toxicology

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Section: Seasonal Variations Of Tolerance Measurementssupporting

confidence: 47%

Section: 2mentioning

confidence: 77%

Section: 2mentioning

confidence: 80%

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Impact of an urban multi-metal contamination gradient: Metal bioaccumulation and tolerance of river biofilms collected in different seasons

Faburé

Dufour²,

Autret

et al. 2015

Aquatic Toxicology

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“…Induced tolerance is observed by comparing the sensitivity of natural communities to a particular contaminant or contaminant mixture (Kim-Tiam et al 2014), using short-term toxicity tests performed under controlled conditions. These tests are based on representative endpoints such as, photosynthetic efficiency (Dorigo et al 2010;Tlili et al 2010), respiration potential (Dorigo et al 2010;) enzymatic activities (Fechner et al 2012), and metabolic capacities (Boivin et al 2005). Different studies have thus highlighted an increased tolerance of microbial communities to various substances such as metals and metalloids, and organic pesticides following chronic exposure to these toxicants in various ecosystems (see for reviews Blanck 2002;Imfeld and Vuilleumier 2012;.…”

Section: Introductionmentioning

confidence: 99%

Improved short-term toxicity test protocol to assess metal tolerance in phototrophic periphyton: toward standardization of PICT approaches

Lambert

Pesce

Foulquier

et al. 2014

Environ Sci Pollut Res

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Pollution-induced community tolerance (PICT) approaches involve comparing tolerance levels of natural communities to a particular contaminant or a contaminant mixture using short-term toxicity tests performed under controlled conditions. However, results from toxicity tests can be modulated by various environmental and experimental conditions, raising questions about their reproducibility and comparability. In this context, the present study aimed to determine the influence of exposure duration, periphyton suspension concentration, and periphyton maturation stage on the measurement of short-term effects of copper on phototrophic periphyton communities. Our results showed the very weak influence of exposure duration in the tested range (2-6 h) on toxicity level, whereas periphyton biomass in the tested suspension (in terms of both chlorophyll a concentrations and dry weight), proved a crucial determinant in toxicity assessment. Results also highlighted the potential tolerance increase with the periphyton maturation stage. This parameter conditioned the positive linear relationship between tolerance level and periphyton suspension concentration, leading to an increase in the linear regression slope with the maturation stage. This suggests that such a relationship is probably highly periphyton-dependent. Consequently, to enable data toxicity comparisons, an a priori normalization of the periphyton suspension biomass is necessary, and PICT approaches require the use, as much of possible, of periphyton with similar maturation stage. Finally, the present study clearly shows that a better standardization of PICT approaches could help to improve reproducibility. It could thus facilitate the comparison of tolerance levels measured in the same study (e.g., spatial and/or temporal and/or inter-treatment comparison) as well as the comparison obtained from different experimental and in situ research.

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“…Community structure can also be assessed by identifying and enumerating taxa present on the CES. DNA-based molecular approaches are increasingly used to elucidate the composition of biofilm communities (Hoellein et al, 2010;Tlili et al, 2011;Fechner, Dufour & Gourlay-Franc e, 2012). Specific ecosystem functions (e.g.…”

Section: Endpointsmentioning

confidence: 99%

A novel method to assess effects of chemical stressors on natural biofilm structure and function

et al. 2015

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SUMMARY1. The regulation and management of chemical contaminants rarely use community-and ecosystemlevel endpoints, partly due to a lack of suitable methods. To overcome this limitation, we propose contaminant exposure substrata (CES), an adaptation of the widely used nutrient-diffusing substratum method, to assess responses of biofilm communities to chemical contaminants in situ. 2. We describe methods for using CES to assess effects on biofilm biomass, community structure, process rates, biofilm-consumer interactions and biofilm chemistry. We also provide equations to calculate the flux of soluble chemicals from CES and describe an approach to compare contaminant dose in CES assays to the contaminant dose encountered by biofilms in polluted surface waters. 3. Data from four case studies demonstrate that CES can detect impairment of biofilm structure and function. 4. The adaptability, simplicity and cost-effectiveness of CES make them valuable tools to assess community-and ecosystem-level responses to contaminants, suggesting potential for routine use and incorporation of data generated from such assays into contaminant regulation and management.

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Pollution-induced community tolerance of freshwater biofilms: measuring heterotrophic tolerance to Pb using an enzymatic toxicity test

Cited by 7 publications

References 23 publications

Impact of an urban multi-metal contamination gradient: Metal bioaccumulation and tolerance of river biofilms collected in different seasons

Impact of an urban multi-metal contamination gradient: Metal bioaccumulation and tolerance of river biofilms collected in different seasons

Improved short-term toxicity test protocol to assess metal tolerance in phototrophic periphyton: toward standardization of PICT approaches

A novel method to assess effects of chemical stressors on natural biofilm structure and function

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