Pollution from azithromycin-manufacturing promotes macrolide-resistance gene propagation and induces spatial and seasonal bacterial community shifts in receiving river sediments

Milaković, Milena; Vestergaard, Gisle; González-Plaza, Juan José; Petrić, Ines; Šimatović, Ana; Senta, Ivan; Kublik, Susanne; Schloter, Michael; Smalla, Kornelia; Udiković-Kolić, Nikolina

doi:10.1016/j.envint.2018.12.050

Cited by 78 publications

(43 citation statements)

References 69 publications

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“…Prior work illustrates that macrolide resistance is among the most prevalent in the wider environment, representing a rich source of resistance determinants with strong potential for horizontal transfer ( 32 ). Our detection of a core airway resistome dominated by macrolide resistance is therefore of particular concern considering the selective pressure exerted by macrolides toward resistance in both the airway and wider environment ( 30 , 33 , 34 ). Recent work focused on environmental resistomes illustrates their distinct core and discriminatory elements ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease

Aogáin¹,

Lau

Cai

et al. 2020

Am J Respir Crit Care Med

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Rationale: Long-term antibiotic use for managing chronic respiratory disease is increasing; however, the role of the airway resistome and its relationship to host microbiomes remains unknown. Objectives: To evaluate airway resistomes and relate them to host and environmental microbiomes using ultradeep metagenomic shotgun sequencing. Methods: Airway specimens from 85 individuals with and without chronic respiratory disease (severe asthma, chronic obstructive pulmonary disease, and bronchiectasis) were subjected to metagenomic sequencing to an average depth exceeding 20 million reads. Respiratory and device-associated microbiomes were evaluated on the basis of taxonomical classification and functional annotation including the Comprehensive Antibiotic Resistance Database to determine airway resistomes. Co-occurrence networks of gene–microbe association were constructed to determine potential microbial sources of the airway resistome. Paired patient-inhaler metagenomes were compared ( n = 31) to assess for the presence of airway–environment overlap in microbiomes and/or resistomes. Measurements and Main Results: Airway metagenomes exhibit taxonomic and metabolic diversity and distinct antimicrobial resistance patterns. A “core” airway resistome dominated by macrolide but with high prevalence of β-lactam, fluoroquinolone, and tetracycline resistance genes exists and is independent of disease status or antibiotic exposure. Streptococcus and Actinomyces are key potential microbial reservoirs of macrolide resistance including the ermX , ermF , and msrD genes. Significant patient-inhaler overlap in airway microbiomes and their resistomes is identified where the latter may be a proxy for airway microbiome assessment in chronic respiratory disease. Conclusions: Metagenomic analysis of the airway reveals a core macrolide resistome harbored by the host microbiome.

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Section: Discussionmentioning

confidence: 99%

Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease

Aogáin¹,

Lau

Cai

et al. 2020

Am J Respir Crit Care Med

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“…Microbial communities in the sediment of receiving streams were previously found to be influenced by the effluent of WWTP discharge and stream water composition (Milaković et al, 2019). From the point of discharge to around 3.5 km downstream, the microbial diversity of the sediment remained relatively stable because most of the microorganisms in the stream water were still adapted to the Y-WWTP environment.…”

Section: Discussionmentioning

confidence: 99%

Behaviors of Homologous Antibiotic Resistance Genes in a Cephalosporin WWTP, Subsequent WWTP and the Receiving River

Lei

Zhang

Ning

et al. 2021

Front. Environ. Sci.

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High concentrations of antibiotics in antibiotic production wastewater can cause the widespread transmission of antibiotic resistance genes (ARGs). Here, we collected a set of time series samples from a cephalosporin production wastewater treatment plant (X-WWTP), the subsequent municipal WWTP (Y-WWTP) and the receiving stream. Using a functional gene microarray, GeoChip 5.0, which contains multiple homologous probes for 18 ARG and 13 antibiotic metabolism gene (AMG) families, we found that more than 50% of homologous probes for 20 gene families showed a relative abundance higher in X-WWTP, while only 10–20% showed lower relative abundance. The different response patterns of homologous ARG (hARGs) within the same ARG family imply environmental selection pressures are only responsible for the ARG enrichment and spread of some specific instead of all ARG-containing microorganisms, which contradicted the traditionally held belief that environmental selection pressures, especially antibiotic concentration, select for all ARG-containing microorganisms thereby selecting different hARGs in the same ARG family in an undifferentiated way. Network results imply that hARGs from three β_lactamase families enriched under the selection pressure of high cephalosporin antibiotic concentrations in X-WWTP formed positively correlated homologous ARG clusters (pohARGCs). The pohARGCs were also enhanced in the sediment of the receiving stream. The enrichment of hARGs from three β_lactamase families was likely through microorganisms belonging to the Betaproteobacteria genus.

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“…The limits of detection and quantification of each antibiotic are featured in Table 2. The four antibiotics investigated in this research work were selected according to their potential risk for the environment and reported occurrence [11][12][13][14][15][16][17][18][19][20][21]. All of them are representative human-use and veterinary-use antibiotics belonging to the main antibiotic groups.…”

Section: Antibiotic Characterizationmentioning

confidence: 99%

Towards the Removal of Antibiotics Detected in Wastewaters in the POCTEFA Territory: Occurrence and TiO2 Photocatalytic Pilot-Scale Plant Performance

Moles¹,

Mosteo²,

Gómez

et al. 2020

Water

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This research aims to assess the presence of four antibiotic compounds detected in the influent and effluent of wastewater treatment plants (WWTPs) in the POCTEFA territory (north of Spain and south of France) during the period of 2018–2019, and to relate the removal of antibiotic compounds with the processes used in the WWTPs. The performance of a photocatalytic TiO2/UV-VIS pilot-scale plant was then evaluated for the degradation of selected antibiotics previously detected in urban treated effluent. The main results reflect that azithromycin had the highest mass loadings (11.3 g/day per 1000 inhabitants) in the influent of one of the selected WWTPs. The results also show considerable differences in the extent of antibiotics removal in WWTPs ranging from 100% for sulfadiazine to practically 0% for trimethoprim. Finally, the photocatalytic TiO2/UV-VIS pilot-scale plant achieved the removal of the four antibiotics after 240 min of treatment from 78%–80% for trimethoprim and enrofloxacin, up to 100% for amoxicillin, sulfadiazine and azithromycin. The catalyst recovery via mechanical coagulation–flocculation–decantation was almost total. The Ti concentration in the effluent of the TiO2/UV-VIS pilot-scale plant was lower than 0.1% (w/w), and its release into the environment was subsequently minimized.

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Pollution from azithromycin-manufacturing promotes macrolide-resistance gene propagation and induces spatial and seasonal bacterial community shifts in receiving river sediments

Cited by 78 publications

References 69 publications

Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease

Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease

Behaviors of Homologous Antibiotic Resistance Genes in a Cephalosporin WWTP, Subsequent WWTP and the Receiving River

Towards the Removal of Antibiotics Detected in Wastewaters in the POCTEFA Territory: Occurrence and TiO2 Photocatalytic Pilot-Scale Plant Performance

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