Pollen Viability Study of Solanum erianthum D. Don

Ghosh, Soumi; Mondal, Tustu; Roy, Anjalika

doi:10.32381/jpsr.2020.36.1-2.34

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings were supported by observations that pollen viability values determined by the TTC staining assay gave consistent results of pollen germination in banana ( Soares et al . 2016 ) and Solanum erianthum ( Ghosh et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Pollen viabilities and gene expression profiles across Musa genomes

Mingmanit,

Boonsrangsom,

Sujipuli

et al. 2023

AoB PLANTS

View full text Add to dashboard Cite

Banana (Musa spp.) is a major global economic fruit crop. However, cross-pollination from other Musa cultivars grown in nearby plantations results in seeded fruit that exceeds market demand. This study investigated pollen viability and germination and examined the expression profiles of pollen development-related genes across seven Musa genomes (AA, BB, AAA, BBB, AAB, ABB and ABBB). Twenty-three Musa cultivars were assessed for pollen viability using LAO and TTC staining methods. Results revealed that pollen viability obtained from both methods was significantly different among all the studied cultivars. Cultivars carrying BB (diploid) genomes had higher viability percentages than AA (diploid), AAA, BBB, AAB and ABB (triploid) and ABBB (tetraploid) genomes. Germination of the studied cultivars was also investigated on pollen culture medium, with results showing significant differences between the pollen of each cultivar. The best germinating cultivar was TKM (11.0%), carrying BB genome. Expression profiles of pollen development-related genes by RT-qPCR indicated that both TPD1A and MYB80 genes were highly expressed in triploid Musa genomes but the PTC1 gene showed down-regulated expression, resulting in non-viable pollen. Pollen viability, pollen germination and pollen development-related genes differed across Musa cultivars. This knowledge will be useful for the selection of male parents for Musa cross-breeding programs. Pollen viability should also be considered when planning Musa production to avoid seeded fruit.

show abstract

Section: Discussionmentioning

confidence: 99%