Pollen Viability in Rosa

Pearson, Hugh M.; Harney, Patricia M.

doi:10.21273/hortsci.19.5.710

Cited by 8 publications

(4 citation statements)

References 6 publications

(10 reference statements)

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“…These data were consistent with the results of Luo et al. (2020) in Castanea mollissima pollen grains and Pearson and Harney (1984) in Rosa pollen grains. There were no significant differences between the pollen viability estimated from the TTC test and the pollen germination.…”

Section: Discussionsupporting

confidence: 92%

“…The pollen viability indicated by red ink and fluorescein diacetate staining was somewhat higher than the actual pollen germination. These data were consistent with the results of Luo et al (2020) in Castanea mollissima pollen grains and Pearson and Harney (1984) in Rosa pollen grains. There were no significant differences between the pollen viability estimated from the TTC test and the pollen germination.…”

Section: Pollen Germination and Pollen Staining Testssupporting

confidence: 93%

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Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Jia

Wang²,

Mi³

et al. 2022

Front. Plant Sci.

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Pollen morphology, pollen vigor, and long-term pollen storage are critical for plant cross-breeding and genetic improvement of Exochorda racemosa. We developed a protocol for viability determination and storage of E. racemosa pollen for breeding new varieties. The medium components for E. racemosa pollen germination was optimized by using an Orthogonal Assay Test Strategy (OATS). The germination rates of E. racemosa pollen were investigated after storing at different temperatures and different storage periods. The size of E. racemosa pollen was medium with three germination ditches, and the sculptural type of pollen was striate. Red ink and 2,3,5-triphenyl tetrazolium chloride (TTC) can effectively distinguish viable pollen from the unviable pollen of E. racemosa. The most suitable medium (CK2) for E. racemosa was composed of 150 g· L–1 sucrose, 100 mg·L–1 boric acid, 150 mg· L–1 Ca(NO3)2 and 50 mg· L–1 GA3. Low-temperature stress produced the greater inhibition of pollen tube growth compared with high-temperature conditions. The CK2 medium at pH 6.5 resulted in the highest pollen germination rate and most extended pollen tube length. The optimal temperature for storage of dried pollen was –80°C (P < 0.01), and the germination rate was 53.60% after storage for 390 days. Thawing in a 35°C water bath produced the best viability of E. racemosa pollen after storage at –20°C and –80°C. The short-term storage of E. racemosa fresh pollen at 4°C was better than that at –20°C and –80°C (P < 0.01). It is possible to evaluate pollen quality and store pollen grains for E. racemosa by the parameters defined in this study.

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Section: Discussionsupporting

confidence: 92%

Section: Pollen Germination and Pollen Staining Testssupporting

confidence: 93%

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Jia

Wang²,

Mi³

et al. 2022

Front. Plant Sci.

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“…In a study by Ilgın et al (2007), pollen viability was higher than that of pollen germination. This result was con sistent with previous studies (Pearson and Harney, 1984;Bolat and Pirlak, 1999;Stanley and Linskens, 2012). Vego and Miljković (2012) found that the best conditions for pollen germination of caprifig were in a medium containing 3% sucrose and 0.01% boric acid solution at a temperature of 30°C in the dark.…”

Section: Introductionsupporting

confidence: 91%

Evaluation of viability and germination of pollen grains of three local caprifig cultivars and their effect on some characteristics of fig fruits (Ficus carica L.)

Barakat,

Draie

2024

Adv. Hort. Sci.

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This research was carried out in fig fields in the village of Kafar-Jales, in the north of Syria. The analyses were conducted in the laboratories of the Faculty of Agriculture at Idlib University during the 2022 farming season, to evaluate the viability and germination of three local varieties of Caprifig (Bunduqi, Azraq, Panjani). The morphological characteristics, the date of the exodus of insects (Blastophaga psenes) from male fruits, the quantity of pollen, and the percentage of germination and viability were studied on four nutritive media at a temperature of 27°C. The measurements were taken after 24 h, 48 h, and 72 h. These three pollinators were used to pollinate three varieties of edible figs (white Satehi, Safrawi, and Habashi) to study the effect of pollen source on the productive characteristics of edible fig fruits. The result showed significant superiority of the Bunduqi cultivar over the rest of the cultivars in traits of the fruit’s early ripening, pollen quantity, and date of departure of insects. The Azraq cultivar is found to be superior to Bunduqi and Panjani cultivars in the germination rate of pollen at all stages of the experiment and in all used media. The addition of stigma flower extract in culture media increased germination percentage by 35% for all studied Caprifig cultivars. The addition of 10% sucrose in the cultivation environment increased the percentage of pollen germination by 20%. The cultivars that were inoculated with the Panjani pollinator outperformed the characteristics of the length, diameter, and weight, demonstrating that the fruit’s quality characteristics are affected by the genotype of the pollinator used. The results of this research can be beneficial for both fig growers and plant breeders, as they help to select the best pollinators and contribute to the development and improvement of the quality of the cultivated fig fruits.

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“…Acetocarmine has been widely used to stain pollen grains in fruit crops (Pham et al, 2015;Wang et al, 2015) and indicate the presence of enzymes or cytoplasm. Higher pollen variability was reported in roses (Pearson and Harney, 1984), pomegranate (Sharma and Gaur, 1984), jatropha (Lyra et al, 2011), dwarf guava (Nongyai et al, 2015), and litchi where 1% acetocarmine was considered more suitable for pollen viability assessment (Gupta et al, 2018). In the current study, the use of 1% acetocarmine dye properly stained the pollens for viability assessment in guava cultivars and pollens of white flesh Round cultivar showed higher viability at all cold treatment time intervals.…”

Section: Effect Of Cold Treatment and Genotype On Pollen Viabilitysupporting

confidence: 48%

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

Bokhari

2023

PAKJAS

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Androgenesis is reported in a few tree crops such as citrus and papaya; however, there is little information available about guava. Different modified Chu (N6) media and cold pretreatments of floral buds were tested to induce embryogenic calli in the anthers of two white flesh guava cultivars, ‘Round’ and ‘Pyriform’. Four modified Chu (N6) media formulations, i.e., M1 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP, 0.5 mgL-1 zeatin and 5% coconut water), M2 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP), M10 (0.5 mgL-1 2,4-D), and M11 (0.5 mgL-1 2,4-D and 50 mgL-1 PVP) enhanced anther swelling and callus formation. Genotypic variability was found in pollen viability, phenolic exudation, and anther tissue browning after cold pretreatment for 0-72 hrs. intervals. Anthers of ‘Round’ showed more pollen viability across cold pretreatment intervals with less phenolic exudations and tissue browning than ‘Pyriform’. On both M10 and M11 media, anthers with uni-nucleate microspores developed embryogenic calli; however, anthers of ‘Pyriform’ resulted in more calli induction (69.58%) on M10 media, whereas anthers of ‘Round’ developed more calli (66.60%) on M11 media. The proliferating calli in white flesh ‘Round’ and ‘Pyriform’ cultivars and pink flesh ‘Round’ cultivar formed more embryogenic masses upon transfer to growth hormone-free Chu (N6) media under light conditions; however, embryos showed delayed maturity with limited germination. More genotypes shall be explored to enhance embryo germination, and embryogenic media shall be further fine-tuned. This study provides the foundation for the generation of homozygous lines in guava using androgenesis

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Pollen Viability in Rosa

Cited by 8 publications

References 6 publications

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Evaluation of viability and germination of pollen grains of three local caprifig cultivars and their effect on some characteristics of fig fruits (Ficus carica L.)

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

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