Pollen Tube Guidance by Attractant Molecules: LUREs

Okuda, Satoshi; Higashiyama, Tetsuya

doi:10.1247/csf.10003

Cited by 26 publications

(23 citation statements)

References 41 publications

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“…Thus, in total, three different sets of DEFL genes were found, each overrepresented among the transcripts showing that expression character: ES-enriched; MPenriched; and dual gametophyte-enriched. Some members of this family have previously been shown to be expressed in synergids in maize and to function as pollen tube attractants in Torenia [28,[44][45][46][47].…”

Section: Gene Ontology Functional Category Enrichmentmentioning

confidence: 99%

“…Characterization focused on two families with known gametophyte members: DEFENSIN/LURE (DEFL) [44], and Zm Egg Apparatus1 (ZMEA1)-LIKE (EAL) [58][59][60][61]; and two families that had not previously been shown to have gametophyte-expressed members, CLAVATA3-ESR (CLE) [62], and LITTLE ZIPPER (ZPR) [63] (Figures 5 and 6; Additional files 17 and 18).…”

Section: Analysis Of Small Peptide Gene Family Expressionmentioning

confidence: 99%

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Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes

et al. 2014

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Background: Plant gametophytes play central roles in sexual reproduction. A hallmark of the plant life cycle is that gene expression is required in the haploid gametophytes. Consequently, many mutant phenotypes are expressed in this phase. Results:We perform a quantitative RNA-seq analysis of embryo sacs, comparator ovules with the embryo sacs removed, mature pollen, and seedlings to assist the identification of gametophyte functions in maize. Expression levels were determined for annotated genes in both gametophytes, and novel transcripts were identified from de novo assembly of RNA-seq reads. Transposon-related transcripts are present in high levels in both gametophytes, suggesting a connection between gamete production and transposon expression in maize not previously identified in any female gametophytes. Two classes of small signaling proteins and several transcription factor gene families are enriched in gametophyte transcriptomes. Expression patterns of maize genes with duplicates in subgenome 1 and subgenome 2 indicate that pollen-expressed genes in subgenome 2 are retained at a higher rate than subgenome 2 genes with other expression patterns. Analysis of available insertion mutant collections shows a statistically significant deficit in insertions in gametophyte-expressed genes.

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Section: Gene Ontology Functional Category Enrichmentmentioning

confidence: 99%

Section: Analysis Of Small Peptide Gene Family Expressionmentioning

confidence: 99%

Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes

et al. 2014

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“…In this system, it was unambiguously shown that the synergids produce pollen tube attractants (Higashiyama et al, 2001). Recently, the Torenia LURE proteins, secreted cysteine-rich peptides that accumulate in the filiform apparatus of the synergid cells, have been identified as pollen tube attractants (Okuda et al, 2009;Okuda and Higashiyama, 2010). In Arabidopsis, synergids and other embryo sac cells produce a diverse set of cysteine-rich proteins (Jones-Rhoades et al, 2007;Dresselhaus and Márton, 2009;Wuest et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells

et al. 2012

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The directional growth of the pollen tube from the stigma to the embryo sac in the ovules is regulated by pollen-pistil interactions based on intercellular communication. Although pollen tube growth is regulated by the cytoplasmic Ca2+ concentration ([Ca2+]cyt), it is not known whether [Ca2+]cyt is involved in pollen tube guidance and reception. Using Arabidopsis expressing the GFP-based Ca2+-sensor yellow cameleon 3.60 (YC3.60) in pollen tubes and synergid cells, we monitored Ca2+ dynamics in these cells during pollen tube guidance and reception under semi-in vivo fertilization conditions. In the pollen tube growing towards the micropyle, pollen tubes initiated turning within 150 μm of the micropylar opening; the [Ca2+]cyt in these pollen tube tips was higher than in those not growing towards an ovule in assays with myb98 mutant ovules, in which pollen tube guidance is disrupted. These results suggest that attractants secreted from the ovules affect Ca2+ dynamics in the pollen tube. [Ca2+]cyt in synergid cells did not change when the pollen tube grew towards the micropyle or entered the ovule. Upon pollen tube arrival at the synergid cell, however, [Ca2+]cyt oscillation began at the micropylar pole of the synergid, spreading towards the chalazal pole. Finally, [Ca2+]cyt in the synergid cell reached a maximum at pollen tube rupture. These results suggest that signals from the pollen tube induce Ca2+ oscillations in synergid cells, and that this Ca2+ oscillation is involved in the interaction between the pollen tube and synergid cell.

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“…Multiple signaling events have to occur successfully after pollination before the pollen tube can finally join the female gametophyte (Lausser and Dresselhaus 2010;Marton and Dresselhaus 2010;Okuda and Higashiyama 2010). Double fertilization results in two individual fertilization products: the diploid zygote that will develop into the embryo and the triploid nutritive tissue of the endosperm.…”

Section: Cell Death Decisions During Fertilization and Seed Developmentmentioning

confidence: 99%

Control of Programmed Cell Death During Plant Reproductive Development

Olvera-Carrillo

Salanenka

Nowack

2011

Biocommunication of Plants

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Programmed cell death (PCD) is an actively controlled, genetically encoded self-destruct mechanism of the cell. While many forms of PCD have been described and molecularly dissected in animals, we know to date only little about the control of PCD processes in plants. Nevertheless, plant PCD is a crucial component of a plant's reaction to its biotic and abiotic environment and a central theme during plant development. In this chapter, we review the communication events triggering and executing, or preventing, PCD during plant reproductive development. These comprise intracellular communication, as well as signaling between cells and tissues, and the intricate communication between genetically distinct individuals that are necessary for successful plant reproduction.

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Pollen Tube Guidance by Attractant Molecules: LUREs

Cited by 26 publications

References 41 publications

Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes

Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes

Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells

Control of Programmed Cell Death During Plant Reproductive Development

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