Pollen tube behavior in yellow passion fruit following compatible and incompatible crosses

Rêgo, M. M. R do; Rêgo, Elizanilda Ramalho do; Bruckner, Cláudio Horst; Silva, E.A.M. Da; Finger, Fernando L.; Pereira, K. J. C.

doi:10.1007/s001220051531

Cited by 30 publications

(27 citation statements)

References 14 publications

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“…Even with association between systems, the sporophytic system is considered predominant, with the presence of glycoproteins, causing SI reaction on the flower stigma. The rejection of pollen tube grow of Passiflora occurs predominantly in the stigma (RÊGO et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

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Method for Overcoming the Passion Fruit Self-Incompatibility

2016

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-The methodology of self-pollination of passion fruit flowers at anthesis after stigma excision was proposed. This method was compared with self-pollination procedures at anthesis and at the stage of flower bud. Two genotypes (M7 and N9), propagated by cuttings were studied. Self-pollinations were performed at 1 pm, 1 pm and 5 pm or 5 pm. Fruit set (%), fruit mass (g), pulp mass (g), pulp/fruit ratio and seed number per fruit were evaluated and compared to fruits derived from M7 x N9 and N9 x M7 crosses. No fruit set was obtained from self-pollination at anthesis, which confirms the self-incompatibility of both genotypes. Self-pollination after stigma excision was the best procedure, reaching average fruit set of 73.61 % (13 hours) and 81.94 % (double self-pollination at 1 pm and 5 pm). Although two self-pollinations after stigma excision at anthesis is more efficient, performing only one self-pollination at 1 pm requires less labor. The amount of seeds produced per fruit is satisfactory to generate inbred population. Index terms: Passiflora edulis, pollination, selfing, endogamous progenies. MÉTODO PARA SUPERAÇÃO DA AUTOINCOMPATIBILIDADE DO MARACUJAZEIRORESUMO -Foi proposta a metodologia de autopolinização de flores de maracujazeiro no estádio de antese, após a excisão do estigma. Este método foi comparado com os procedimentos de autopolinização na antese e no estádio de botão floral, ambos sem remoção do estigma. Dois genótipos (M7 e N9), propagados por estaquia, foram estudados. As autopolinizações foram realizadas às 13 e 17 h ou às 17 h. Foram avaliadas a frutificação (%), massa do fruto (g), massa da polpa (g), relação polpa/fruto e número de sementes por fruto, e comparadas às características de frutos derivados dos cruzamentos M7 x N9 e N9 x M7. Não houve frutificação da autopolinização no estádio de antese, que confirma a autoincompatibilidade dos dois genótipos estudados. A autopolinização após a excisão do estigma é o melhor procedimento, que atinge médias de frutificação de 73,61 %, com apenas uma autopolinização às 13 h, e 81,94 %, com dupla autopolinização às 13 e 17 h. Contudo, apesar de ser mais eficiente fazer duas autopolinizações, após a excisão do estigma na antese, é menos trabalhoso realizar apenas uma autopolinização às 13 h. A quantidade de sementes produzida por fruto é satisfatória para gerar nova população endogâmica. Termos para indexação: Passiflora edulis, polinização, autofecundação, progênies endogâmicas.

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Section: Resultsmentioning

confidence: 99%

“…) is an allogamous plant due to sporophytic selfincompatibility (BRUCKNER et al, 1995). The incompatible pollen tube growth is inhibited at the stigmatic surface (RÊGO et al, 1999;RÊGO et al, 2000). Suassuna et al (2003) proposed the presence of a gametophytic locus associated with the sporophytic system.…”

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confidence: 99%

Method for Overcoming the Passion Fruit Self-Incompatibility

2016

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“…Due to its considerable economic importance in tropical agricultural systems, sour passion fruit is used as a model species to study the SI mechanisms in Passifloraceae (Bruckner et al 1995;Ręgo et al 2000;Suassuna et al 2003). The molecular basis of SI in passion fruit is unknown, but the plant displays a homomorphic sporophytic SI (SSI) system (Bruckner et al 1995), which is also observed in other families as Brassicaceae, Asteraceae, Sterculiaceae and Convolvulaceae.…”

Section: Introductionmentioning

confidence: 99%

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

et al. 2014

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Self-incompatibility (SI) is a genetic mechanism in angiosperms that prevents selfing. The SI system in passion fruit (Passiflora edulis Sims) was investigated using hand pollinations. Pollen tube growth was inspected by microscopy, and sequence analysis of potential regulators of this process was carried out. The results revealed that the pollen tubes grew slowly and were often completely arrested in the stigma in an incompatible combination. Under these circumstances the pollen tube was rapidly and significantly rearranged, followed by the rapid deposition of callose in the stigma during the SI response. The structural changes in the pollen grain after an incompatible pollination were investigated using scanning electron microscopy. Furthermore, ultrastructural observations during incompatible interactions showed that the membrane system of the pollen tube was damaged, and fertilisation was not observed or was considerably delayed when compared to compatible interactions. The analysis presented here provides evidence that the passion fruit genome presents similar sequences to those encoding factors involved in SI in different species. These results suggest that, in the SI system of passion fruit, the rejection of an incompatible pollen grain is characterised by drastic structural changes in both pollen and pollen tube.

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“…Species of Passiflora L. are characterized by having a sporophytic and gametophytic selfincompatibility system (Rêgo et al 1999(Rêgo et al , 2000Suassuna et al 2003), and the recognition reaction of pollen, as in other angiosperms, occurs mainly on the stigmatic surface (Rêgo et al 2000). The stigma of 1% OsO 4, washed in 0.1 M sodium phosphate buffer at pH 7.2 (Weber 1992), dehydrated in acetone, critical point dried (Gersterberger & Leins 1978), sputter-coated with gold using a Balzers SCD 050, and examined using a Jeol 6060 SEM.…”

Section: Introductionmentioning

confidence: 99%

The formation of the stigmatic surface in Passiflora elegans (Passifloraceae)

Silvério

Mariath

2010

Rodriguésia

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The stigma surface is a complex multicellular structure where the development of the pollen tube begins. This development is necessary for sucess in fertilization and depends on recognition processes that involve the anatomy of the stigma. Passiflora is an economically important genus because of its edible fruits. Many authors have described the stigma of Passiflora but nothing is known about the ontogenesis of this structure. This work aimed to describe the formation of the stigmatic surface of Passiflora elegans. Results showed that, in bud, the stigmatic surface of this species is flat with small cells. The cells in the subdermal layer have large vacuoles and the nucleus, near to the external periclinal walls. During its development the stigma surface becomes uneven due to the elongation of cells in the subdermal layer. Elongation results in an increase of external secretory surface area of the stigmas, and probably plays an important role in pollen recognition. The polysaccharide content found in the inner walls of these structures might be involved in the signal process for pollen tube growth during its early development. The morphological evidence presented here shows that, as the stigma of Passiflora is formed by dermal and subdermal cells, it should not be characterized as colleters or papillae and, therefore, it is defined here as stigma emergences. Key-words: anatomy, stigma development, stigma emergence, pollination. ResumoA superfície estigmática é uma estrutura multicelular complexa, onde o tubo polínico inicia o seu desenvolvimento, necessária para a fecundação. Este desenvolvimento depende de condições favoráveis que envolvem a anatomia do estigma durante o processo de reconhecimento. Passiflora é um gênero economicamente importante devido aos seus frutos comestíveis. O estigma de Passiflora tem sido descrito por vários autores, mas o seu processo de formação é desconhecido. Esse trabalho tem por objetivo descrever o processo de formação da superfície estigmática de Passiflora elegans. Os resultados demonstram que durante a fase de botão jovem, a superfície estigmática é composta por pequenas células e apresenta superfície plana. As células da camada subdepidérmica apresentam grandes vacúolos e núcleo, próximo da parede periclinal externa. Durante o seu desenvolvimento, a superfície estigmática torna-se irregular devido ao alongamento de células da camada subdepidérmica. Essas modificações resultam em um acréscimo da superfície secretora externa do estigma, e provavelmente desempenham um importante papel no reconhecimento do pólen. Os conteúdos polissacarídicos encontrados na superfície interna dessas estruturas podem estar envolvidos com os processos de sinalização do tubo polínico durante seu desenvolvimento inicial. As evidências morfológicas observadas nesse trabalho demonstram que as estruturas presentes na superfície do estigma de Passiflora são constituídas por células de origem dérmica e subdérmica, e não devem ser caracterizadas como coléteres ou papilas, sendo assim, caracterizad...

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Pollen tube behavior in yellow passion fruit following compatible and incompatible crosses

Cited by 30 publications

References 14 publications

Method for Overcoming the Passion Fruit Self-Incompatibility

Method for Overcoming the Passion Fruit Self-Incompatibility

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

The formation of the stigmatic surface in Passiflora elegans (Passifloraceae)

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