Pollen sterols—a mass spectrographic survey

Standifer, L. N.; Devys, Michel; Barbier, Michel

doi:10.1016/s0031-9422(00)85638-1

Cited by 47 publications

(25 citation statements)

References 13 publications

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“…However, some plants display particular pollen sterolic profile with large absolute amounts of less conventional sterols such as cholestenone in the pollen of S. fragilis . This has been already shown for the pollen of heather ( Calluna vulgaris , Ericaceae) that contains large amount of stigmasterol, as well as for the pollen of cottonwood ( Populus fremontii , Salicaceae) and cat's ear ( Hypochoeris radicata , Asteraceae) (Standifer et al, ) that both display high level of cholesterol.…”

Section: Discussionmentioning

confidence: 72%

“…Although S. caprea and S. fragilis display similar sterolic composition as also observed for species belonging to Rosaceae and, to a lesser extent, to Boraginaceae; Standifer et al () found little evidence that taxonomy could be used to predict pollen sterol profile since three species belonging to Salicaceae (e.g., Populus genus, Salix genus) varied widely in the content of C 27 , C 28, and C 29 sterols. This sterolic diversity is not limited to pollen but concerns all plant parts.…”

Section: Discussionmentioning

confidence: 86%

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Generalized host‐plant feeding can hide sterol‐specialized foraging behaviors in bee–plant interactions

Vanderplanck

Zerck

Lognay

et al. 2019

Ecology and Evolution

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Host-plant selection is a key factor driving the ecology and evolution of insects.While the majority of phytophagous insects is highly host specific, generalist behavior is quite widespread among bees and presumably involves physiological adaptations that remain largely unexplored. However, floral visitation patterns suggest that generalist bees do not forage randomly on all available resources. While resource availability and accessibility as well as nectar composition have been widely explored, pollen chemistry could also have an impact on the range of suitable host-plants. This study focuses on particular pollen nutrients that cannot be synthesized de novo by insects but are key compounds of cell membranes and the precursor for molting process: the sterols. We compared the sterol composition of pollen from the main hostplants of three generalist bees: Anthophora plumipes, Colletes cunicularius, and Osmia cornuta, as well as one specialist bee Andrena vaga. We also analyzed the sterols of their brood cell provisions, the tissues of larvae and nonemerged females to determine which sterols are used by the different species. Our results show that sterols are not used accordingly to foraging strategy: Both the specialist species A. vaga and the generalist species C. cunicularius might metabolize a rare C 27 sterol, while the two generalist species A. plumipes and O. cornuta might rather use a very common C 28 sterol. Our results suggest that shared sterolic compounds among plant species could facilitate the exploitation of multiple host-plants by A. plumipes and O. cornuta whereas the generalist C. cunicularius might be more constrained due to its physiological requirements of a more uncommon dietary sterol. Our findings suggest that a bee displaying a generalist foraging behavior may sometimes hide a sterol-specialized species. This evidence challenges the hypothesis that all generalist free-living bee species are all able to develop on a wide range of different pollen types. K E Y W O R D Sbee-flower interactions, generalization, insects, physiological constraints, sterols | 151 VANDERPLANCK Et AL.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 86%

Generalized host‐plant feeding can hide sterol‐specialized foraging behaviors in bee–plant interactions

Vanderplanck

Zerck

Lognay

et al. 2019

Ecology and Evolution

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“…The pollen of most plant species accumulate intermediates such as 24-methylenecholesterol and 9β,19-cyclopropyl sterols [30]. However, sitosterol can be the main sterol in some species, as found in Baccharis viminea, Juniperus uthaensis, Polygonum sp., Hydrophyllum capitatum, Pinus sylvestris, and other plants [31]. Large amounts of stigmasterol and cholesterol were found in the pollen of heather (Calluna vulgaris) and in cottonwood (Populus fremontii), respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The main Psyche sterol present in the pollen of plants is 24-methylenecholesterol [16][17][18]. However, other studies reported that besides the 24-methylenecholesterol, the pollen presents phytosterols as desmosterol, campesterol, fucosterol, sitosterol, and even small quantities of cholesterol [17].…”

Section: Introductionmentioning

confidence: 99%

Neutral Sterols of Cephalic Glands of Stingless Bees and Their Correlation with Sterols from Pollen

Ferreira-Caliman

Silva

Mateus

et al. 2012

Psyche: A Journal of Entomology

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Sterols are essential molecules in the membrane lipid composition and precursors of important sterol hormones that regulate many developmental processes. Insects are unable to synthesize sterols de novo and, thus, all phytophagous insects depend on an exogenous source of sterols for growth, development, and reproduction. The sterol requirements of social bees are not fully known due to the fact that there is no well-defined diet available throughout the year with regard to floral resources. Our study aimed to characterize the sterols present in pollen stored in Melipona marginata and Melipona scutellaris colonies, as well as evaluating their presence in the mandibular, hypopharyngeal, and cephalic salivary gland secretions. We analyzed the chemical composition of pollen stored in the colonies and the composition of the cephalic glands of workers in three adult functional phases (newly emerged, nurses, and foragers) by gas chromatography and mass spectrometry. The results showed that the pollen analyzed contained campesterol, stigmasterol, sitosterol, isofucosterol, lanosterol, and small amounts of cholesterol. The glands showed the same compounds found in the pollen analyzed, except lanosterol that was not found in M. scutellaris glands. Surprisingly, cholesterol was found in some glands with relative ratios greater than those found in pollen.

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“…Pollen lipids have also been studied (Hisamichi and Abe 1967;Kvanta 1968;Lepage and Boch 1968;and Standifer et al 1968). Previous investigations with pine pollen grains have dealt with development or morphology or both, or they have dealt with physiology or biochemistry or both; but to date, pine pollen investigations have not dealt with morphological investigations of lipid-extracted pollen grains.…”

mentioning

confidence: 99%

Ultrastructural changes caused by lipid extraction of pollen of Pinus echinata

Hess¹,

Weber²,

Allen³

et al. 1973

Can. J. Bot.

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1973. Ultrastructural changes caused by lipid extraction of pollen of PLrus eclrbrcrtn. Can. J. Bot. 51: 1685-1688.Lipid-extracted and non-lipid-extracted Pitrus eclrit~ara Mill. pollen grains were examined by light microscopy, scanning electron microscopy, and freeze-etching and thin-sectioning procedures used with transmission electron microscop Non-l~pid-extracted ollen rains floated in aqueous solutions, sacci were not collapsed, and organeiks were not disrupted: Lipifextracted pollen grains did not float in aqueous solutions, sacci collapsed, and organelles were disrupted. In the vacuum of the scanning electron microscope the sporoderm in the aperture-bearing area collapsed causing sacci to come into contact with each other.

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Pollen sterols—a mass spectrographic survey

Cited by 47 publications

References 13 publications

Generalized host‐plant feeding can hide sterol‐specialized foraging behaviors in bee–plant interactions

Generalized host‐plant feeding can hide sterol‐specialized foraging behaviors in bee–plant interactions

Neutral Sterols of Cephalic Glands of Stingless Bees and Their Correlation with Sterols from Pollen

Ultrastructural changes caused by lipid extraction of pollen of Pinus echinata

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