Pollen shells and soluble factors play non-redundant roles in the development of allergic conjunctivitis in mice

Fukase, Saaya; Ando, Tomoaki; Matsuzawa, Moe; Kimura, Meiko; Sone, Yoshitsugu; Izawa, Kumi; Kaitani, Ayako; Kamei, Anna; Kojima, Mayuki; Nakano, Nobuhiro; Maeda, Keiko; Shimizu, Toshiaki; Ogawa, Hideoki; Okumura, Ko; Nishiyama, Makoto; Murakami, Akira; Ebihara, Nobuyuki; Kitaura, Jiro

doi:10.1016/j.jtos.2021.08.009

Cited by 5 publications

(17 citation statements)

References 65 publications

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“…Thus, we propose that the sialylation is one of the most important factors that regulate Muc5ac gel-forming mucin functions in the conjunctiva. We previously reported that the pollen shells play an essential role in conjunctival local sensitization as well as in the elicitation phase of the allergic conjunctivitis 69 . In addition, raw ragweed pollens have been shown to release sub-pollen particles upon being soaked in water, which in turn enhance the allergic inflammation 55 .…”

Section: Discussionmentioning

confidence: 99%

“…The experimental allergic conjunctivitis models were induced as previously described 69 . For Balb/c background strains, each foot pad of Balb/c or Ao mice received 50 μg of RW pollens emulsified in 50 μL of Imject Alum Adjuvant (ThermoFisher Scientific) on day 0.…”

Section: Allergic Conjunctivitis Modelsmentioning

confidence: 99%

“…7a). For B6J background strains, the local sensitization model was used 69 . Each conjunctival sac received 2.5 μL of 200 mg/mL RW pollen suspension once a day for 5 days per week for 3 weeks.…”

Section: Allergic Conjunctivitis Modelsmentioning

confidence: 99%

“…Mouse eyes were scored for the signs of immediate hypersensitivity responses 30 min after the topical challenges of RW pollen suspension, according to previously published criteria 69 . Conjunctival redness, chemosis, tearing, and eyelid edema were graded as 0 (absent), 1 (mild), 2 (moderate), or 3 (severe) by a person blind to the mouse identity.…”

Section: Evaluation Of Allergic Conjunctivitis Modelmentioning

confidence: 99%

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The protective role of conjunctival goblet cell mucin sialylation

et al. 2023

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Gel-forming mucins secreted by conjunctival goblet cells have been implicated in the clearance of allergens, pathogens, and debris. However, their roles remain incompletely understood. Here we show that human and mouse conjunctival goblet cell mucins have Alcian blue-detectable sialic acids, but not sulfates in the steady state. Interestingly, Balb/c mouse strain lacks this sialylation due to a point mutation in a sialyltransferase gene, St6galnac1, which is responsible for sialyl-Tn synthesis. Introduction of intact St6galnac1 to Balb/c restores the sialylation of conjunctival goblet cell mucus. Sialylated mucus efficiently captures and encapsulates the allergen particles in an impenetrable layer, leading to the protection of mice from the development of allergic conjunctivitis. Expression of ST6GALNAC1 and sialyl-Tn is upregulated in humans under conditions with chronic stimuli. These results indicate that the sialylated glycans on the ocular mucins play an essential role in maintaining the conjunctival mucosa by protecting from the incoming foreign bodies such as allergen particles.

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Section: Discussionmentioning

confidence: 99%

Section: Allergic Conjunctivitis Modelsmentioning

confidence: 99%

Section: Allergic Conjunctivitis Modelsmentioning

confidence: 99%

Section: Evaluation Of Allergic Conjunctivitis Modelmentioning

confidence: 99%

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The protective role of conjunctival goblet cell mucin sialylation

et al. 2023

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“… 111 Furthermore, in a recent study in mice model of OA, repeated topical applications of a mixture of particulate air pollutants and pollen extract induced eosinophilic conjunctivitis suggesting that particulate pollutants probably act as adjuvants in development of allergic conjunctivitis. 112 How pollutants aggravate OA and whether conjunctival epithelial integrity is altered by pollution and/or oxidative stress, is an area of further investigation.…”

Section: Factors Contributing To Ocular Barrier Dysregulation In Oamentioning

confidence: 99%

Epithelial barrier dysfunction in ocular allergy

Singh

Diebold

Sahu

et al. 2021

Allergy

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The epithelial barrier is the first line of defense that forms a protective barrier against pathogens, pollutants, and allergens. Epithelial barrier dysfunction has been recently implicated in the development of allergic diseases such as asthma, atopic dermatitis, food allergy, and rhinitis. However, there is limited knowledge on epithelial barrier dysfunction in ocular allergy (OA). Since the ocular surface is directly exposed to the environment, it is important to understand the role of ocular epithelia and their dysfunction in OA. Impaired epithelial barrier enhances allergen uptake, which lead to activation of immune responses and development of chronic inflammation as seen in allergies. Abnormal expression of tight junction proteins that helps to maintain epithelial integrity has been reported in OA but sufficient data not available in chronic atopic (AKC) and vernal keratoconjunctivitis (VKC), the pathophysiology of which is not just complex, but also the current treatments are not completely effective. This review provides an overview of studies, which indicates the role of barrier dysfunction in OA, and highlights how ocular barrier dysfunction possibly contributes to the disease pathogenesis. The review also explores the potential of ocular epithelial barrier repair strategies as preventive and therapeutic approach.

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