Pollen quality of fresh and 1-year-old single pollen diets for worker honey bees (<i>Apis mellifera</i>L.)

Pernal, Stephen F.; Currie, R. William

doi:10.1051/apido:2000130

Cited by 185 publications

(186 citation statements)

References 77 publications

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“…The protein content of the pollen diets used in our experiment (16.5 and 25.2 % of proteins in the BLow^and BHigh^diets, respectively) can be considered representative of different nutritional scenarios, and is similar to nutritional reference values used in other studies (Di Pasquale et al 2013;Pernal and Currie 2000;Standifer et al 1970). Our results confirm the positive effect of a high-protein diet on HPG size for bees sampled the 8th day, which corresponds to the age of maximum development of HPGs in worker bees (Al-Ghamdi et al 2011;Lass and Crailsheim 1996).…”

Section: Discussionsupporting

confidence: 85%

Combined effect of pollen quality and thiamethoxam on hypopharyngeal gland development and protein content in Apis mellifera

et al. 2016

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-Pollen diet is essential to nurse bees to develop hypopharyngeal glands (HPGs) and produce royal jelly to feed larvae. The effect of different diet regimes combined with pesticide exposure on the development of HPGs in honeybees was studied. In particular, a high-quality pollen mixture (high pollen diversity and protein content) and a low-quality diet (low pollen diversity and protein content) were combined with the chronic administration of thiamethoxam (10 and 40 μg/L). The impact of these stressors was evaluated by measuring the size of HPG acini and the total protein content of bee heads in 8-and 12-day-old bees. Bees fed with sub-lethal concentrations of thiamethoxam and with the low-quality diet exhibited smaller and irregularly shaped acini. The total protein content in bee head was also affected by pesticide administration, while the pollen quality did not cause significant modifications. In conclusion, these results highlighted the importance of the food quality, considered as nutritional value and pesticide contamination, for the physiological development of honeybees.HPGs / honeybee / neonicotinoid / pollen diet / chronic toxicity

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Section: Discussionsupporting

confidence: 85%

Combined effect of pollen quality and thiamethoxam on hypopharyngeal gland development and protein content in Apis mellifera

et al. 2016

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“…HPG development (Pernal & Currie 2000), suggesting that IC in bees is not sensitive to the amount of protein. However, we cannot exclude the possibility that other pollen with poorer or richer protein content or challenges with pathogens would induce differences in IC.…”

Section: Discussionmentioning

confidence: 99%

“…The honeybee is a valuable model for such studies, because they usually pollinate a variety of plants but sometimes are forced to feed on single crops (monocultures). In addition, pollen, which is the main source of dietary protein and contains essential amino acids for their development (de Groot 1953), can influence longevity, the development of hypopharyngeal glands (HPG) and ovaries (Pernal & Currie 2000) and the susceptibility to pathogens (Rinderer et al 1974;Rinderer & Elliott 1977); but to what extent pollen can affect IC is not known. Finally, honeybee populations have been declining over the last years and a current idea suggests that honeybee colonies may suffer from a compromised immune system (van Engelsdorp et al 2008), which could be related to poor nutrition commonly associated with colony losses (van Engelsdorp et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Diet effects on honeybee immunocompetence

Alaux¹,

Ducloz²,

Crauser³

et al. 2010

Biol. Lett.

658

540

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The maintenance of the immune system can be costly, and a lack of dietary protein can increase the susceptibility of organisms to disease. However, few studies have investigated the relationship between protein nutrition and immunity in insects. Here, we tested in honeybees (Apis mellifera) whether dietary protein quantity (monofloral pollen) and diet diversity (polyfloral pollen) can shape baseline immunocompetence (IC) by measuring parameters of individual immunity (haemocyte concentration, fat body content and phenoloxidase activity) and glucose oxidase (GOX) activity, which enables bees to sterilize colony and brood food, as a parameter of social immunity. Protein feeding modified both individual and social IC but increases in dietary protein quantity did not enhance IC. However, diet diversity increased IC levels. In particular, polyfloral diets induced higher GOX activity compared with monofloral diets, including protein-richer diets. These results suggest a link between protein nutrition and immunity in honeybees and underscore the critical role of resource availability on pollinator health.

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“…Samples contaminated with fat body or gut contents were discarded, and clear samples were dispensed into siliconized microcentrifuge tubes (Thermo Fisher Scientific, Waltham, MA, USA) containing 100 μL of HPLC-grade methanol (Sigma-Aldrich, St. Louis, MO, USA) and stored at − 40°C to permit multiple samples to be analyzed in the same assay run. To determine ovarian status, whole ovaries were removed and rinsed with saline, suspended in 2-3 mL of saline, then assessed using a 5-point scale (Pernal and Currie 2000), in which 0 indicates ovarioles with no signs of swelling, 4 indicates queen-like ovarioles with elongated ooyctes, and intermediate scores are assigned based on degree of swelling and presence of constrictions between follicles, following standard methods (Rueppell et al 2011). Both ovaries were inspected, and a score was given based on the most advanced development observed.…”

Section: Hemolymph and Tissue Collectionsmentioning

confidence: 99%

“…Heavy selection for behavioral traits may result in loss or masking of subtle aspects of physiological regulation. Also, many studies that describe ovarian activation typically report low scores, rarely exceeding a 1 (slight swelling of the ovarioles) or 2 (moderate swelling of the ovarioles) on the Pernal and Currie (2000) scale. Other investigators have taken a more conservative approach in classifying only ovarioles with developed ooyctes and clear signs of yolk deposition as activated (Malka et al 2007).…”

Section: Ecdysteroid Titers Were Unrelated To Ovary Activation and Ovmentioning

confidence: 99%

Queen mandibular pheromone modulates hemolymph ecdysteroid titers in adult Apis mellifera workers

Trawinski

Fahrbach

2018

Apidologie

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-To test the hypothesis that exposure to queen mandibular pheromone (QMP) modulates ecdysteroid production in adult worker honey bees, ecdysteroids were measured in hemolymph and other tissues of individual adult worker honey bees reared with or without QMP in cages and field colonies. Ecdysteroid titers were higher in caged workers exposed to QMP continuously from the first day of adult life than in workers reared without QMP. Statistical cluster analysis suggested the possibility that a subgroup of workers (Bresponders^) is more sensitive to QMP in this regard than other workers. In 12-day-old workers, ecdysteroid titers in workers reared in queenright (QR) colonies were similar to those observed in cages with QMP, but lower than those in queenless (QL) colonies. Differences in number of ovarioles or degree of ovarian activation did not correlate with hemolymph ecdysteroids. Previous studies have demonstrated ecdysteroids in hemolymph in very young adult workers and in workers in QL colonies; the present study indicates that production of ecdysteroids occurs in older adult worker honey bees in the absence of morphological signs of ovarian activation, with cage studies revealing a modulatory role for QMP masked in the complex environment of the hive.ecdysteroids / ovary / queen mandibular pheromone / queenless / queenright

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Pollen quality of fresh and 1-year-old single pollen diets for worker honey bees (Apis melliferaL.)

Cited by 185 publications

References 77 publications

Combined effect of pollen quality and thiamethoxam on hypopharyngeal gland development and protein content in Apis mellifera

Combined effect of pollen quality and thiamethoxam on hypopharyngeal gland development and protein content in Apis mellifera

Diet effects on honeybee immunocompetence

Queen mandibular pheromone modulates hemolymph ecdysteroid titers in adult Apis mellifera workers

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