Pollen morphology as fertility predictor in hybrid tea roses

Pipino, Luca; Labeke, Marie-Christine Van; Mansuino, Andrea; Scariot, Valentina; Giovannini, A.; Leus, Leen

doi:10.1007/s10681-010-0298-5

Cited by 21 publications

(20 citation statements)

References 29 publications

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“…Unlike many other cultivated plant species (Kilian et al, 2007; Gil-Ariza et al, 2009; Wang et al, 2010; Gross et al, 2014), we did not see a reduction over time when we considered the year of release of the variety. This may corroborate observations on the sensitivity of rose for inbreeding depression, which makes selfing unsuitable as a breeding strategy (Pipino et al, 2011). It might also be the result of the large range of ornamental traits that breeders have selected for, causing selection to never be consistently in the same direction.…”

Section: Discussionsupporting

confidence: 86%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

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Petal color is one of the key characteristics determining the attractiveness and therefore the commercial value of an ornamental crop. Here, we present the first genome-wide association study for the important ornamental crop rose, focusing on the anthocyanin and carotenoid contents in petals of 96 diverse tetraploid garden rose genotypes. Cultivated roses display a vast phenotypic and genetic diversity and are therefore ideal targets for association genetics. For marker analysis, we used a recently designed Axiom SNP chip comprising 68,000 SNPs with additionally 281 SSRs, 400 AFLPs and 246 markers from candidate genes. An analysis of the structure of the rose population revealed three subpopulations with most of the genetic variation between individual genotypes rather than between clusters and with a high average proportion of heterozygous loci. The mapping of markers significantly associated with anthocyanin and carotenoid content to the related Fragaria and Prunus genomes revealed clusters of associated markers indicating five genomic regions associated with the total anthocyanin content and two large clusters associated with the carotenoid content. Among the marker clusters associated with the phenotypes, we found several candidate genes with known functions in either the anthocyanin or the carotenoid biosynthesis pathways. Among others, we identified a glutathione-S-transferase, 4CL, an auxin response factor and F3'H as candidate genes affecting anthocyanin concentration, and CCD4 and Zeaxanthine epoxidase as candidates affecting the concentration of carotenoids. These markers are starting points for future validation experiments in independent populations as well as for functional genomic studies to identify the causal factors for the observed color phenotypes. Furthermore, validated markers may be interesting tools for marker-assisted selection in commercial breeding programmes in that they provide the tools to identify superior parental combinations that combine several associated markers in higher dosages.

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Section: Discussionsupporting

confidence: 86%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

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“…Some literatures have postulated that figs control seed loss though variation in style length [14–18]. In some plants such as fig ( Ficus maxima ), it is believed that stigma length shows an allometric relationship with style length.…”

Section: Discussionmentioning

confidence: 99%

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Valdiani

Kadir

Saad³

et al. 2012

The Scientific World Journal

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The ambiguity of crossability in Andrographis paniculata (AP) was pointed out in the present research. Accordingly, the effects of different style length and crossing time on intraspecific crossability of seven AP accessions in 21 possible combinations were investigated. The best results came out between 08:00 to 11:00 h for manual out-crossing of AP, while the time from 12:00 to 18:00 h showed a decreasing trend. Moreover, 12 mm style length was found as the most proper phenological stage in terms of stigmatic receptivity to perform out-crossing in this plant. All in all, AP behaved unlikely in each combination, and a significant difference was observed in crossability of AP accessions (P < 0.01). The lowest and highest crossability rate was found in hybrids 21 (11261NS × 11344K) and 27 (11322PA × 11350T) with 0.25% and 13.33%, respectively. Furthermore, a significant negative relationship between style length and crossibility (r 2 = 0.762∗∗) was recorded in this research. As a final conclusion, crossing time and proper style length can improve the intraspecific crossability in the species, considerably. Despite all the mentioned contrivances, we still believe that a genetic incongruity should be involved as an additional obstacle in crossability of those combinations that failed or responded deficiently to outcrossing.

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“…Changes in pollen viability and germination rate during storage at -20 °C For this study, five cultivars ('Golden Fashion', 'Dolphin', 'Swan', 'Touch of Class', 'New Fashion') with high fertility pollen, based on their diameter larger than 30 µm (Pipino et al 2011b), were selected; these cultivars were also suggested by NIRP International, provider of the used material, as cultivars with high potential for breeding programs. The pollen aspect during TTC coloration and pollen grain germination is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Prolonged Cold Storage Affects Pollen Viability and Germination along with Hydrogen Peroxide and Nitric Oxide Content in <i>Rosa hybrida</i>

MACOVEI¹,

Caser²,

DONÀ³

et al. 2016

Not Bot Hort Agrobot Cluj

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Roses (Rosa hybrida) are the most important ornamental cut-flowers and breeders' main focus is to develop new desirable modern cultivars. Rose breeding programs center on the introduction of new flower colors, thornless stems, higher production and good post-harvest performance. The study of the main pollen traits, such as pollen quantity and quality, viability, longevity, morphological homogeneity, germination and tube growth, is important for building suitable breeding programs. Recently, a number of studies have shown that reactive oxygen species, like hydrogen peroxide, and nitric oxide, are involved in a wide range of signaling processes including pollen tube growth and pollen-pistil recognition. Pollen viability after anther dehiscence is crucial for successful crossbreeding. In the present work, pollen grains from 5 hybrid tea rose cultivars were stored at -20 °C up to 12 months. Pollen viability and germination rate was monitored in order to provide useful information about pollen storage length. Additionally, pollen grains were tested for their content in hydrogen peroxide and nitric oxide by using a novel approach where the fluorescence is read in a quantitative RealTime PCR (qRT-PCR) machine. Pollen viability and in vitro pollen germination capacity varied among the rose genotypes, while a progressive decrease was evidenced during 12 months of storage at low temperature. Both hydrogen peroxide and nitric oxide production were found to be genotypedependent, whilst accumulation of the two molecules was observed during the storage period. A putative detrimental effect of these molecules during pollen conservation is hypothesized.

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Pollen morphology as fertility predictor in hybrid tea roses

Cited by 21 publications

References 29 publications

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Prolonged Cold Storage Affects Pollen Viability and Germination along with Hydrogen Peroxide and Nitric Oxide Content in <i>Rosa hybrida</i>

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