Pollen grain size in four ploidy levels of genusAvena

Katsiotis, Andreas; Forsberg, R. A.

doi:10.1007/bf01678036

Cited by 32 publications

(29 citation statements)

References 7 publications

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“…A strong correlation between pollen grain length, grain width and pore diameter can be noted. In earlier studies on Poaceae pollen grain size a correlation between pollen grain length (longest axis) and pollen grain width (axis orthogonal to previous mentioned axis) has already been shown in Avena sativa (Katsiotis and Forsberg 1995). Our results confirm this observation.…”

Section: Discussionsupporting

confidence: 91%

“…However, thresholds in parameter size differ between the studies and no definite criteria for discrimination emerge which could be useful for all study areas. In a study on Avena sativa Katsiotis and Forsberg (1995) discovered that the grain size increases with the ploidy level. Additionally, the authors found a positive correlation between major and minor axis of the grains.…”

Section: Morphological Differentiation Of Pollen Grainsmentioning

confidence: 99%

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Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

Schüler

Behling

2010

Veget Hist Archaeobot

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Despite the dominance of grasslands during the last glacial period, especially in South America, the highly uniform morphology of Poaceae pollen grains has so far allowed only very few palynological studies based on Poaceae pollen. In our study we compare two methods of distinguishing between South American grassland ecosystems based on quantitative morphology of Poaceae pollen grains. We investigated data sets from Páramo in southern Ecuador, Campos de Altitude and Campos in south-eastern and southern Brazil as well as data sets from the Pampa in Argentina by measuring the pollen grain length, grain width, pore diameter and annulus width. Firstly we investigated the potential influence of chemical treatment of pollen grains on pollen grain size as well as the measurement setting for defining the boundary conditions for using Poaceae pollen grains in a palaeoecological investigation. Finally the measured pollen grain parameters were analyzed by comparison of average grain length using statistical tests. This approach reveals highly significant differences in average grain size between all grassland ecosystems. Assuming that a certain grain size range can be assigned to a certain Poaceae taxon, conclusions about differences and similarities in taxa composition can be derived. We used two methods of multivariate data analysis. One uses the pollen grain parameters directly for a Principle Component Analysis (PCA). The other is an already established method in grassland ecology which defines parameter based pollen grain types to investigate similarities between grassland ecosystems. Both approaches confirm the results of the grain length analysis. In this work we demonstrate that the method we developed has the potential to provide acquisition of so far inaccessible information on spatial and temporal patterns and dynamics of South American grasslands.

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Section: Discussionsupporting

confidence: 91%

Section: Morphological Differentiation Of Pollen Grainsmentioning

confidence: 99%

Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

Schüler

Behling

2010

Veget Hist Archaeobot

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“…cause an increase of cell size in plants what could lead to an increase of the size of some organs, like flowers and seeds. The positive relationship between ploidy level and pollen size has been reported for several plant genera either in natural or artificially induced polyploids (Katisiotis andForsberg 2005, Celenk et al 2008).…”

Section: Resultsmentioning

confidence: 74%

Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

Sousa

Pierre

Torres

et al. 2013

An. Acad. Bras. Ciênc.

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The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri-and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia may be used as an additional taxonomic character of the genus.

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“…O tamanho do grão de pólen em várias espécies tem relação com o nível de ploidia (Raseira & Raseira, 1996). A relação positiva entre o nível de ploidia e tamanho do pólen tem sido relatada para vários gêneros de plantas poliploides, como Avena L. (Katsiotis & Forsberg, 1995), Mentha L. (Celenk et al 2008) e Lippia L. (Sousa et al 2013). Conforme Simioni et al (2004) plantas que produzem grãos de pólen com 30% a 40% de incremento no tamanho, quando comparados com grãos considerados normais, servem como um indicativo de grãos de pólen não reduzidos.…”

Section: Acessosunclassified

Estimativa da viabilidade polínica de araçazeiro (Psidium cattleianum Sabine) através de distintos métodos de coloração

Hister

Tedesco

2016

Rev. bras. plantas med.

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RESUMO:A análise histoquímica dos grãos de pólen através do uso de corantes permite a determinação de sua viabilidade polínica. O objetivo deste estudo foi comparar a eficiência dos corantes orceína acética 2% e reativo de Alexander modificado, para estimar a viabilidade polínica de vinte acessos de araçazeiro (Psidium cattleianum Sabine) -uma árvore de frutos muito apreciados que também é utilizada para fins medicinais. Botões florais na pré-antese foram coletados e fixados em etanol:ácido acético (3:1) por 24 horas, após os botões foram transferidos para etanol 70% e mantidos sob refrigeração. A técnica de esmagamento das anteras foi utilizada no preparo das lâminas. Para cada acesso foram preparadas 2 lâminas por corante e analisados 500 grãos de pólen por lâmina. A viabilidade polínica foi estimada através da porcentagem de grãos de pólen viáveis. De modo geral, observou-se que os grãos de pólen corados com orceína acética 2% apresentaram viabilidade alta, acima de 98,1%, e os acessos não diferiram entre si. A viabilidade polínica através da coloração com a solução de Alexander variou de 43% (acesso Silveira Martins 1) a 97% (acesso Candelária 1), mostrando que provavelmente exista variabilidade genética entre os acessos. Em apenas dois acessos não houve diferença significativa entre os valores de viabilidade encontrados através dos dois corantes testados. Conclui-se então que a orceína acética 2% superestimou a viabilidade polínica de P. cattleianum e que o reativo de Alexander é o mais preciso, devido a sua dupla coloração (verde de malaquita + fucsina ácida). Assim, indica-se o reativo de Alexander para análise de estimativa da viabilidade polínica de genótipos de araçá envolvidos em um programa de melhoramento genético. Palavras-chave:Araçá, orceína acética, reativo de Alexander, viabilidade polínica ABSTRACT: Estimation of pollen viability of strawberry guava (Psidium cattleianum Sabine) through distinct staining methods. The histochemical analysis of pollen grains through the use of stains makes it possible to determine their pollen viability. The purpose of this study was to compare the efficiency of 2% acetic orcein and Alexander's stain modified, in estimating the pollen viability of twenty strawberry guava accessions (Psidium cattleianum Sabine) -a valued fruit tree which is used for medicinal purposes. Pre-anthesis flower buds were collected and fixed in ethanol:acetic acid (3:1 v/v) for 24 hours at room temperature, subsequently they were stored in 70% ethanol under refrigeration. The anther squash technique was performed for slide preparation. In each accession two slides per stain and 500 pollen grains were analyzed. The pollen viability was estimated according to the percentage of viable pollen grains. Overall, it was observed that pollen grains stained with 2% acetic orcein evinced a high viability, higher than 98,1%, and the accessions did not differ. The pollen viability estimated with Alexander's stain varied from 43% (accession of Silveira Martins 1) to 97% (accession of Candelária 1), in...

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Pollen grain size in four ploidy levels of genusAvena

Cited by 32 publications

References 7 publications

Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

Estimativa da viabilidade polínica de araçazeiro (Psidium cattleianum Sabine) através de distintos métodos de coloração

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