Pollen flow within and among isolated populations of two rare, self-compatible plant species from inselbergs of Northeast Brazil

Wanderley, Artur Maia; Santos, Eloyza Karoline Rozendo dos; Galetto, Leonardo; Benko‐Iseppon, Ana Maria; Machado, Isabel Cristina

doi:10.1007/s11258-020-01004-5

Cited by 8 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To quantify self-pollination and geitonogamy in wild populations, we tracked the flow of the pollen with pollen-substitutes. Flowers of 10 randomly chosen individuals were dusted with UV-reflective dust (RadGlo RC 33, Radiant Color, Europark 1046 B 3530 Houthalen) on their inner surface of the corolla near to and on the anthers, similarly to Wanderley et al (2020). At night, a UV emitting torch (MorPilot, 51 Led) was used for checking the inner corolla of all flowers on the same stalk as the source UV flower and on all individual plants occurring within one meter radius from the dusted, source flower.…”

Section: Nectar Production and Sugar Concentrationmentioning

confidence: 99%

Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes

et al. 2021

View full text Add to dashboard Cite

Plant mating systems may reflect an adaptation to a habitat type, with self-pollination being potentially common in unstable and disturbed conditions. We investigated the reproductive ecology of an Alpine, narrow-range toadflax, Linaria tonzigii Lona (Plantaginaceae), occurring in steep and dynamic mountain screes. We explored self-compatibility and spontaneous autogamy, seed viability, daily nectar production, pollinator behaviour and pollen transfer in wild populations, using hand pollination treatments, quantification of nectar volume, viability Tetrazolium assay, active pollinator sampling and video recordings, and UV-bright dust for pollen substitution. After ex novo sequencing of several genetic regions of L. tonzigii, we performed a multi-marker phylogenetic analysis of 140 Linaria species and tracked the occurrence of the self-compatibility trait. Our results showed that this species is self-compatible, pollinated mostly via spontaneous autogamy and pollinator-mediated geitonogamy, and self-pollinated seeds are as viable as cross-pollinated ones. Selfing could be due to pollinator rarity because, despite the studied species providing a high nectar volume, wild bees, moths and small beetles infrequently visited its flowers in the sparsely vegetated scree slopes. In addition, a preliminary survey showed a low genetic haplotype diversity in the study plant. Moreover, the phylogeny shows that self-compatibility is scattered in the tree, suggesting the adaptive nature of this reproductive trait in the genus Linaria. This study supports for theories that high prevalence of selfing is an adaptation to environments unfavourable for cross-pollination, and particularly where pollinators are rare, such as in perturbed, poorly vegetated high-elevation habitats.

show abstract

Section: Nectar Production and Sugar Concentrationmentioning

confidence: 99%

Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Because of the differences in corolla colour and lip-shape between these species, it is likely that this diversity is linked to specific hummingbird pollinators, but extensive field observations will be needed to better understand this relationship. Wanderley et al (2020) studied pollination success of A. manoelfelixii and A. pernambucense in isolated populations and showed that both species selfpollinate. However, pollination by hummingbirds increased fecundity.…”

Section: Discussionmentioning

confidence: 99%

“…This species is pollinated by hummingbirds (Wanderley et al 2020). It has been collected with flowers and fruits between March and August.…”

Section: Ecologymentioning

confidence: 99%

An overview of the Brazilian inselberg genus Ameroglossum (Linderniaceae, Lamiales), with the description of seven new species

Almeida

Christenhusz

Wanderley

et al. 2021

EJT

992

View full text Add to dashboard Cite

Field studies of the inselberg flora of north-eastern Brazil have resulted in the discovery of much greater morphological diversity of the genus Ameroglossum, than previously envisaged. These enigmatic plants are endemic to isolated rock outcrops, which have long been surrounded by unsuitable habitat. The morphological forms are geographically isolated and stable in cultivation when grown under similar conditions. We here provide detailed descriptions of the two previously known species and propose an additional seven new species in the genus. A taxonomic revision of the genus, including an identification key is provided. We hypothesize that the evolution of this genus is driven by the diversity of their hummingbird pollinators. Due to the threatened nature of inselberg habitats and the small populations found for most taxa, the species described here are likely to be under threat and in need for formal redlisting.

show abstract

“…The same PCR conditions were applied as described in the original publications for each primer pair. Electrophoresis of the PCR products was carried out on denaturing 6% polyacrylamide (PAA) gels on an automated sequencer (Li-Cor 4300 IR 2 ) following the procedure described by Wagner et al (2015).…”

Section: Methodsmentioning

confidence: 99%

“…An indirect estimate of pollen versus seed flow among plant populations can be obtained by comparing the relative extent of nuclear versus chloroplast genetic differentiation (Ennos 1994). Whereas chloroplast markers are maternally inherited via seeds in most angiosperms and presumably also in bromeliads (Wagner et al 2015), nuclear markers are biparentally inherited and are able to move via both pollen and seed flow.…”

mentioning

confidence: 99%

Genetic structure and gene flow among populations of Encholirium magalhaesii, a rocky grassland fields bromeliad

et al. 2020

View full text Add to dashboard Cite

Encholirium is a genus of 31 xerophytic terrestrial or epilithic bromeliad species endemic to eastern Brazil. Together with Dyckia and Deuterocohnia, it forms the so-called xeric clade of the Pitcairnioideae subfamily of Bromeliaceae. Encholirium species are adapted to rocky landscapes, and many of them are found in the rocky grassland fields of the Espinhaço in the state of Minas Gerais, which is considered the diversity center of the genus. Encholirium species and other bromeliads growing on isolated, island-like habitats like rock outcrops and "inselbergs" are often characterized by high levels of genetic structure and limited gene flow among populations, but there are also exceptions. In the present study, we applied nuclear and chloroplast microsatellite markers to investigate the genetic diversity and population structure of E. magalhaesii L.B.Sm., an endemic species of the Espinhaço, collected in four localities of the Diamantina municipality in southeastern Brazil. Moderate genetic differentiation among localities was revealed by the nuclear markers (F st = 0.199), whereas genetic structuring was much higher with plastid markers (F st = 0.496). Estimations of pollen/seed flow ratios suggest that pollen flow is more efficient in maintaining the genetic connectivity among E. magalhaesii populations than seed flow.

show abstract

Pollen flow within and among isolated populations of two rare, self-compatible plant species from inselbergs of Northeast Brazil

Cited by 8 publications

References 28 publications

Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes

Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes

An overview of the Brazilian inselberg genus Ameroglossum (Linderniaceae, Lamiales), with the description of seven new species

Genetic structure and gene flow among populations of Encholirium magalhaesii, a rocky grassland fields bromeliad

Contact Info

Product

Resources

About