Pollen Cryobanking for Tropical Plant Species

Rajasekharan, P. E.; Ravish, B S; Kumar, T. V. Suresh; Ganeshan, S.

doi:10.1007/978-1-4614-3776-5_4

Cited by 12 publications

(6 citation statements)

References 10 publications

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“…Cryopreserving the pollen of male sterile lines for use on female lines can save large amounts of land in hybrid seed production fields and labour to collect pollen during busy periods of hybridisation. Pollen of many tropical plant species has successfully been cryopreserved: for example, the Indian Institute of Horticultural Research in Bangalore holds 650 samples of pollen from 45 species belonging to 15 plant families [ 219 ].…”

Section: Approaches To Germplasm Conservationmentioning

confidence: 99%

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Pathirana

Carimi

2022

Plants

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Despite the dramatic increase in food production thanks to the Green Revolution, hunger is increasing among human populations around the world, affecting one in nine people. The negative environmental and social consequences of industrial monocrop agriculture is becoming evident, particularly in the contexts of greenhouse gas emissions and the increased frequency and impact of zoonotic disease emergence, including the ongoing COVID-19 pandemic. Human activity has altered 70–75% of the ice-free Earth’s surface, squeezing nature and wildlife into a corner. To prevent, halt, and reverse the degradation of ecosystems worldwide, the UN has launched a Decade of Ecosystem Restoration. In this context, this review describes the origin and diversity of cultivated species, the impact of modern agriculture and other human activities on plant genetic resources, and approaches to conserve and use them to increase food diversity and production with specific examples of the use of crop wild relatives for breeding climate-resilient cultivars that require less chemical and mechanical input. The need to better coordinate in situ conservation efforts with increased funding has been highlighted. We emphasise the need to strengthen the genebank infrastructure, enabling the use of modern biotechnological tools to help in genotyping and characterising accessions plus advanced ex situ conservation methods, identifying gaps in collections, developing core collections, and linking data with international databases. Crop and variety diversification and minimising tillage and other field practices through the development and introduction of herbaceous perennial crops is proposed as an alternative regenerative food system for higher carbon sequestration, sustaining economic benefits for growers, whilst also providing social and environmental benefits.

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Section: Approaches To Germplasm Conservationmentioning

confidence: 99%

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Pathirana

Carimi

2022

Plants

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“…by a stream of dry air (Buitink, et al 1996), and WC in dehydration-sensitive cells remain above the critical points, the time in which cells pass through intermediate WC ranges is shorter. Thus, fast drying reduces the overall occurrence of metabolism-derived damages (Farrant, et al 1993;Kioko, et al 1998;Pammenter and Berjak 1999;Pammenter, et al 2002;Pritchard and Manger 1998). Wheat pollen lost viability very fast upon slow drying, even before any physicochemical change in the water properties was detected.…”

Section: Desiccation Damages Prior Cooling Can Be Reduced By Rapid Dryingmentioning

confidence: 99%

Fast Drying Combined With Rapid Cooling Can Enhance The Survival of The Desiccation Sensitive Wheat Pollen After Ultra-Low Temperature Storage

Impe

Ballesteros

Nagel

2021

Preprint

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Long-term storage of pollen is important for the fertilization of spatially or temporally isolated female parents, especially during hybrid breeding. Wheat pollen is dehydration-sensitive and rapidly loses viability after shedding. To preserve wheat pollen, we hypothesized that fast-(flash)-drying and fast cooling (150°C min-1) compared to slow-(air)-drying and slow cooling (1°C min-1) would increase the rate of intracellular water content (WC) removal, decrease intracellular ice crystal formation, and increase viability after exposure to ultra-low temperatures. High correlations were found between pollen WC and viability analyzed by impedance flow cytometry (IFC viability: r=0.92, P<0.001) and pollen germination (r=0.94, P<0.001). After 10 min of air-drying, 66% WC was lost and pollen germination was at 12.2±12.3%. After 10 min of flash-drying, WC of pollen reduced by 74%. IFC viability decreased from 90.2±6.7 to 39.4±17.9%, and pollen germination dropped from 33.7±16.9 to 1.9±3.9%. After 12 min of flash-drying, WCs decreased to <0.34 mg H2O mg-1 DW, ice crystal formation was completely prevented (ΔH=0 J mg-1 DW), and pollen germination reached 1.2±1.0%. After slow and fast cooling, flash-dried pollen (WC 0.91±0.11 mg H2O mg-1 DW) showed less ice crystal formation during cryomicroscopic-video-recordings and had IFC viability of 4.5±7.0% (slow) and 6.1±8.8% (fast), respectively, compared to air-dried pollen which lost all viability. Generally, fast-(flash)-drying and increased cooling rates may enable the survival of wheat pollen likely due to (1) a fast rate of intracellular WC loss that reduces deleterious biochemical changes associated with the drying process and (2) a delay and reduction in intracellular ice crystal formation.

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“…The only source of information on the cryopreservation of potato and hop pollen is the United States Department of Agriculture (USDA), which has a germplasm repository for these crops and has performed some DSC studies of pollen for cryopreservation, and for other plant species [ 28 , 29 , 30 , 31 , 32 ]. On the other hand, there are quite a lot of papers involved in pollen cryopreservation, carried out on more than 170 plant species [ 33 ], including tropical species [ 34 ], exceptional plants [ 35 ] and medicinal and ornamental plant species [ 36 , 37 ], and vegetable crops [ 25 ]. Cryopreservation can be a suitable tool for preserving the genetic resources of plants, and also for increasing the efficiency of the breeding program [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding

Faltus,

Domkářová,

Svoboda

et al. 2024

Plants

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This study investigated the thermal properties of potato and hop pollen for cryopreservation and subsequent cross-breeding. Phase transitions and frozen water content in selected pollen samples were measured using a differential scanning calorimeter (DSC). Unlike hop pollen, potato pollen showed high variability in thermal properties and water content. Three specific types of pollen samples based on their thermal characteristics and water content were distinguished by DSC in potato: (1) ‘glassy’, with a water content lower than 0.21 g water per g dry matter; (2) ‘transient’, with a water content between 0.27 and 0.34 g of water per g of dry matter; (3) ‘frozen’, with a water content higher than 0.34 g of water per g of dry matter. Only the ‘glassy’ pollen samples with a low water content showed suitable properties for its long-term storage using cryopreservation in potato and hops. Cryopreservation of pollen did not significantly reduce its viability, and cryopreserved pollen was successfully used to produce both potato and hop hybrids. The results indicate that cryopreservation is a feasible technique for the preservation and utilization of pollen of these crops in the breeding process.

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Pollen Cryobanking for Tropical Plant Species

Cited by 12 publications

References 10 publications

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Management and Utilization of Plant Genetic Resources for a Sustainable Agriculture

Fast Drying Combined With Rapid Cooling Can Enhance The Survival of The Desiccation Sensitive Wheat Pollen After Ultra-Low Temperature Storage

Analysis of Thermal Characteristics of Potato and Hop Pollen for Their Cryopreservation and Cross-Breeding

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