Pollen as a target of environmental changes

Grover, Anil; Twell, David; Schleiff, Enrico

doi:10.1007/s00497-016-0285-7

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…Analysing plant cells and in particular the quality of pollen in an efficient and reliable manner is not only of interest of plant breeding and seed production industries, but plays also a role in scientific projects investigating pollen development [30,50], pollen-related heat or cold tolerance [22,23], male-sterility systems [51], the establishment of pollen storage or doubled haploid protocols [13,14]. The most critical argument against all methods analysing pollen viability is the missing relevance for pollen germination [39].…”

Section: Discussionmentioning

confidence: 99%

“…The applied combination of controlled and adjustable temperature gradient and IFC analysis opens opportunities to screen for genetic variation in temperature tolerance [22,23]. …”

Section: Discussionmentioning

confidence: 99%

“…In brief, the developmental steps are accompanied by changes in cell size, composition of the cytoplasm, cell wall formation, nuclear divisions, and metabolic activity, but seem to occur not necessarily in a highly synchronised manner [17–19]. The carbohydrate type and content is species dependent [20], varies during the development [21], is affected by stress [22,23] and storage conditions [24] which further affects the membrane protective function of sucrose [25] with consequences for pollen viability and later germination capacity.…”

Section: Introductionmentioning

confidence: 99%

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Impedance Flow Cytometry: A Novel Technique in Pollen Analysis

Heidmann

Schade-Kampmann²,

Lambalk

et al. 2016

PLoS ONE

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IntroductionAn efficient and reliable method to estimate plant cell viability, especially of pollen, is important for plant breeding research and plant production processes. Pollen quality is determined by classical methods, like staining techniques or in vitro pollen germination, each having disadvantages with respect to reliability, analysis speed, and species dependency. Analysing single cells based on their dielectric properties by impedance flow cytometry (IFC) has developed into a common method for cellular characterisation in microbiology and medicine during the last decade. The aim of this study is to demonstrate the potential of IFC in plant cell analysis with the focus on pollen.MethodDeveloping and mature pollen grains were analysed during their passage through a microfluidic chip to which radio frequencies of 0.5 to 12 MHz were applied. The acquired data provided information about the developmental stage, viability, and germination capacity. The biological relevance of the acquired IFC data was confirmed by classical staining methods, inactivation controls, as well as pollen germination assays.ResultsDifferent stages of developing pollen, dead, viable and germinating pollen populations could be detected and quantified by IFC. Pollen viability analysis by classical FDA staining showed a high correlation with IFC data. In parallel, pollen with active germination potential could be discriminated from the dead and the viable but non-germinating population.ConclusionThe presented data demonstrate that IFC is an efficient, label-free, reliable and non-destructive technique to analyse pollen quality in a species-independent manner.

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Section: Discussionmentioning

confidence: 99%

“…The applied combination of controlled and adjustable temperature gradient and IFC analysis opens opportunities to screen for genetic variation in temperature tolerance [22,23]. …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impedance Flow Cytometry: A Novel Technique in Pollen Analysis

Heidmann

Schade-Kampmann²,

Lambalk

et al. 2016

PLoS ONE

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“…Growing evidences show that reproductive phase is the most vulnerable stage to extreme heat stress in most of the crops. There is dire need to find out heatresistant germplasm for the tailored breeding, for better understanding of pollen development and fertilization and to illustrate reaction of reproductive tissues under high temperature stress (Grover et al, 2016). Various heat-tolerant plant species have been evolved via process of natural selection through attaining acclimation and adjustment mechanism.…”

Section: Future Perspectives For Application Of Modern Tools For Improving Crops Geneticsmentioning

confidence: 99%

Response and tolerance mechanism of food crops under high temperature stress: a review

et al. 2022

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High temperature stress events are critical factors inhibiting crop yield. Meanwhile, world population is growing very rapidly and will be reached up to 9 billion by 2050. To feed increasing world population, it is challenging task to increase about 70% global food productions. Food crops have significant contribution toward global food demand and food security. However, consequences from increasing heat stress events are demolishing their abilities to survive and sustain yield when subjected to extreme high temperature stress. Therefore, there is dire need to better understand response and tolerance mechanism of food crops following exposure to heat stress. Here, we aimed to provide recent update on impact of high temperature stress on crop yield of food crops, pollination, pollinators, and novel strategies for improving tolerance of food crop under high temperature stress. Importantly, development of heat-resistant transgenic food crops can grant food security through transformation of superior genes into current germplasm, which are associated with various signaling pathways as well as epigenetic regulation in response to extreme high temperature stress.

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“…Accuracy of different viability test varies with species or cultivars (Rodriguez-Riano and Dafni, 2000). In vitro pollen germination test is a very effective and convenient method to study the applied aspects of pollen biology (Kristen and Kappler, 1990;Grover et al, 2016). Viability and germination test of pollens have been reported in litchi and other several sapindaceous plant species.…”

Section: Issn: 2319-7706 Volume 6 Number 7 (2017) Pp 270-278mentioning

confidence: 99%