Pollen Allergens: Molecular Cloning and Mechanism for Pollen-induced Asthma

Knox, Bruce; Smith, Penelope M. C.; Suphioglu, Cenk; Taylor, Philip; Avjioglu, Asil; Theerakulpisut, Piyada; Hough, T.; Ong, Eng Kok; Singh, Mohan B.

doi:10.1007/978-1-4612-2958-2_2

Cited by 3 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…␣-Expansin proteins are found in low abundance even in rapidly growing tissues in which they are specifically expressed; they are not readily soluble in solutions of low ionic strength, are not glycosylated, and are tightly bound to cell walls (9,19,20). In contrast, group I allergens are found in high abundance in pollen, are highly soluble in dilute solutions, are glycosylated, and apparently do not bind tightly to the pollen wall (21,22). These differences suggested to us that the function of the group I allergens may be to loosen the cell walls of the stigma and style to allow penetration of the pollen tube through these tissues.…”

Section: Resultsmentioning

confidence: 99%

Group I allergens of grass pollen as cell wall-loosening agents

Cosgrove

Bedinger

Durachko³

1997

Proc. Natl. Acad. Sci. U.S.A.

316

243

View full text Add to dashboard Cite

Group I allergens are the major allergens of grass pollen, but their biological function is unknown. These proteins are shown here to be structurally related to expansins, which are able to induce extension (creep) of plant cell walls. Extracts of maize pollen possess potent expansinlike activity, as measured in wall extension and wall stressrelaxation assays. This activity is selective for grass cell walls and is, at least partly, due to the action of maize group I allergens. We propose that group I allergens facilitate invasion of the pollen tube into the maternal tissues by loosening the cell walls of the grass stigma and style. Additionally, the presence of related mRNAs in vegetative tissues of rice, Arabidopsis, and soybean implies that allergen homologs may function to loosen walls in growing vegetative tissues as well.

show abstract

Section: Resultsmentioning

confidence: 99%

Group I allergens of grass pollen as cell wall-loosening agents

Cosgrove

Bedinger

Durachko³

1997

Proc. Natl. Acad. Sci. U.S.A.

316

243

View full text Add to dashboard Cite

show abstract

“…Although the natural function o f these organ-specific pollen proteins that promote allergic reactions is not known with certainty [1], they are considered to be involved in pol len/stigma recognition mechanisms and thus regulate the plant breeding system [2], Environmental factors that affect growing conditions for plants including soil fertility, mois ture, light, and temperature have been shown to affect pollen size, pollen production, pollen viability, germination, and seedling heterogeneity [3][4][5][6][7][8][9][10], It is expected that an im proved understanding of environmental effects on pollen al lergen synthesis would increase understanding of relation ships between environmental factors and variation in atmo spheric content of pollen allergens.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of Pollen Proteins within Individual <i>Betula Pendula</i> Trees

Hjelmroos

Schumacher²,

Hage‐Hamsten³

1995

Int Arch Allergy Immunol

View full text Add to dashboard Cite

To determine the variation of antigenic water soluble proteins in white birch (Betula pendula) pollen, extracts of pollen from different sides of individual trees were analyzed by isoelectric focusing (IEF), crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis. IgE-antibody-binding patterns were also studied in samples analyzed by IEF by probing with serum pooled from patients with birch pollen allergy, followed by radiolabelled anti-IgE. Antigenic proteins and allergens per unit weight of extracted protein were greatest in pollen extracts from the south side of the trees. Allergens decreased progressively in pollen from west- through east- to north- facing branches. Proteins with high isoelectric points (pI > 8.5) and proteins between pI 4.5 and 5.6 were infrequent in extracts from the north side. Extracts from branches facing north were poor in allergens: in general, only one or two precipitin lines were found, and in some cases they did not bind to IgE antibodies. Differences between numbers of proteins and allergens found in extracts from south, west and north branches were statistically significant for all methods used. The results indicate the need to collect birch pollen for allergen extract manufacture from south-facing branches.

show abstract

“…[5, 6] In the subtropical high altitude zones of Brazil, Lolium multiflorum (Italian or annual ryegrass; subfamily: Pooideae) has been shown to be a major sensitizing pollen source in patients with grass pollen allergy. [7–9] However, also other grass species, such as Anthoxanthum odoratum (sweet vernal grass; subfamily: Pooideae), Cynodon dactylon (Bermuda grass; subfamily: Chloridoideae), Holcus lanatus (common velvet grass; subfamily: Pooideae), Paspalum notatum (bahia grass; subfamily: Panicoideae) and Bromus sp (subfamily: Pooideae) occur there.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that most clinically relevant grasses belong to the Pooideae subfamily, although in certain climatic and geographic areas such as the Mediterranean area and areas with subtropical climate, grasses from other families, such as Bermuda grass ( Cynodon dactylon; subfamily: Chloridoideae), may also play an important role. [ 5 , 6 ] In the subtropical high altitude zones of Brazil, Lolium multiflorum (Italian or annual ryegrass; subfamily: Pooideae) has been shown to be a major sensitizing pollen source in patients with grass pollen allergy. [ 7 – 9 ] However, also other grass species, such as Anthoxanthum odoratum (sweet vernal grass; subfamily: Pooideae), Cynodon dactylon (Bermuda grass; subfamily: Chloridoideae), Holcus lanatus (common velvet grass; subfamily: Pooideae), Paspalum notatum (bahia grass; subfamily: Panicoideae) and Bromus sp (subfamily: Pooideae) occur there.…”

Section: Introductionmentioning

confidence: 99%

Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients

et al. 2015

View full text Add to dashboard Cite

BackgroundGrass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.ObjectiveTo analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.MethodsWe analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.ResultsWithin the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%), Phl p 5 (82%), Phl p 2 (76%) followed by Phl p 4 (64%), Phl p 6 (45%), Phl p 11 (18%) and Phl p 12 (18%). Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.ConclusionsComponent-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population.

show abstract

Pollen Allergens: Molecular Cloning and Mechanism for Pollen-induced Asthma

Cited by 3 publications

References 14 publications

Group I allergens of grass pollen as cell wall-loosening agents

Group I allergens of grass pollen as cell wall-loosening agents

Heterogeneity of Pollen Proteins within Individual <i>Betula Pendula</i> Trees

Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients

Contact Info

Product

Resources

About