Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

Oita, Iuliana; Halewyck, Hadewych; Pieters, Sigrid; Thys, Bert; Heyden, Yvan Vander; Rombaut, Bartholomeus

doi:10.1016/j.jviromet.2012.05.015

Cited by 13 publications

(13 citation statements)

References 32 publications

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“…It is even worse when using Nano-ESI-MS for the analysis of a single cell owing to its very small sample volume. [10,15] Although the separation methods, such as liquid chromatograph (LC), [22][23][24][25] capillary electrophoresis (CE) [1,7,[26][27][28][29] and solid phase micro-extraction (SPME), [30][31] have been frequently applied for salt removal to reduce the interference from the matrix, the process is relatively tedious. Moreover, the separation procedures for LC or CE may cause a sample dilution during elution, especially for ultra-small volume of samples.…”

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confidence: 99%

Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

et al. 2013

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confidence: 99%

Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

et al. 2013

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“…The use of the surfactant SDS as BGE additive allows to overcome the common troubles of irreproducibility encountered in the CZE analyses of non‐enveloped icosahedral viruses . Although BGE additives may not be necessary in some cases , when the presence of SDS is required, it becomes problematic to understand the behavior of non‐enveloped icosahedral viruses since the way by which SDS interacts with the surface of such viruses is poorly known.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, non‐enveloped icosahedral viruses possess a global negative surface charge at pH higher than the isoelectric point contributing to proffer them an electrophoretic mobility . Exploiting this characteristic, it has been shown that capillary zone electrophoresis (CZE), a separative analytical technique based on the difference of the electrophoretic mobility of the analytes , can be applied in the separation, detection and quantification of viral, subviral and virus‐like particles in aqueous media . However, in practice, the CZE analysis of viruses is harder than of molecules, where uncontrolled viral particles aggregation and capillary wall adsorption responsible of irreproducible results having been often encountered .…”

Section: Introductionmentioning

confidence: 99%

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

et al. 2017

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SDS is commonly employed as BGE additive in CZE analysis of non-enveloped icosahedral viruses. But the way by which SDS interacts with the surface of such viruses remains to date poorly known, making complicate to understand their behavior during a run. In this article, two related bacteriophages, MS2 and Qβ, are used as model to investigate the migration mechanism of non-enveloped icosahedral viruses in SDS-based CZE. Both phages are characterized by similar size and surface charge but significantly different surface hydrophobicity (Qβ > MS2, where '>' means 'more hydrophobic than'). By comparing their electrophoretic mobility in the presence or not of SDS on both sides of the CMC, we show that surface hydrophobicity of phages is a key factor influencing their mobility and that SDS-virus association is driven by hydrophobic interactions at the surface of virions. The CZE analyses of heated MS2 particles, which over-express hydrophobic domains at their surface, confirm this finding. The correlations between the present results and others from the literature suggest that the proposed mechanism might not be exclusive to the bacteriophages examined here.

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“…However, Oita et al. found the complex salt and impurity composition in cell extracts very challenging to assess the poliovirus concentration [54]. In such condition, a sample preparation procedure using ultrafiltration or chromatography to purify and concentrate the SLPPs is necessary in order to allow injecting enough SLPPs to be above the detection limit and removing the interfering species [54].…”

Section: Analytical Techniques For Rapid Characterization and Quantifmentioning

confidence: 99%

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

Yang

et al. 2020

Engineering in Life Sciences

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Super large proteinaceous particles (SLPPs) such as virus, virus like particles, and extracellular vesicles have successful and promising applications in vaccination, gene therapy, and cancer treatment. The unstable nature, the complex particulate structure and composition are challenges for their manufacturing and applications. Rational design of the processing should be built on the basis of fully understanding the characteristics of these bio‐particles. This review highlights useful analytical techniques for characterization and stabilization of SLPPs in the process development and product formulations, including high performance size exclusion chromatography, multi‐angle laser light scattering, asymmetrical flow field‐flow fractionation, nanoparticle tracking analysis, CZE, differential scanning calorimetry, differential scanning fluorescence, isothermal titration calorimetry , and dual polarization interferometry. These advanced analytical techniques will be helpful in obtaining deep insight into the mechanism related to denaturation of SLPPs, and more importantly, in seeking solutions to preserve their biological functions against deactivation or denaturation. Combination of different physicochemical techniques, and correlation with in vitro or in vivo biological activity analyses, are considered to be the future trend of development in order to guarantee a high quality, safety, and efficacy of SLPPs.

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Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

Cited by 13 publications

References 32 publications

Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

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