Policosanol as a new inhibitor candidate for vascular calcification in diabetic hyperlipidemic rats

Elseweidy, Mohamed M.; Zein, Nabila; Aldhamy, Samih E; El-Sawy, M. M.; Saeid, Saeid A

doi:10.1177/1535370216659943

Cited by 24 publications

(13 citation statements)

References 38 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 1 h of alloxan administration, a 5% to 10% glucose (alpha chemika) was added to drinking water for 24 h to protect the rats from the hypoglycemic shock. 42–45 Blood glucose level was measured for each rat after 48 h. Rats that achieved a blood sugar level ≥200 mg/dl were selected as diabetic. 46–48 Diabetic rats were fed later on cholesterol diet (CCT) that contains 4% cholesterol (Alpha Chemical Co., India), 1% cholic acid (Sigma Chemical company) mixed with chow diet along with drinking water contain 0.5% thiouracil (Riedel-De Haen Ag Seelize-Hannover, Germany) for a period of 14 days 41,42 and continued later for eight weeks along with the treatment intake.…”

Section: Methodsmentioning

confidence: 99%

Early myocardial injury biomarkers in diabetic hyperlipidemic rats: Impact of 10-dehydrogingerdione and vitamin D3

Elseweidy

Aly

Hammad

et al. 2020

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

Hyperlipidemia represents a major risk factor for cardiovascular diseases leading to myocardial injury. The present study aimed to illustrate the myocardial injury induced in a diabetic hyperlipidemic rat model and the effect of vitamin D3, 10-DHGD intake either individually or in combination form. Male albino rats were selected for the study, received alloxan, hypercholesterolemic diet, and categorized into four groups. The first one (DHC), received hypercholesterolemic diet only and referred to as control. The remaining groups (2, 3, 4) received vitamin D3, 10-DHGD, and combination of both, respectively. Certain biomarkers that were selected for MI evaluation included blood glucose, lipogram pattern, Copeptin, C-reactive protein, myeloperoxidase, heart fatty acid-binding protein, and histopathological changes in myocardium and aorta. Vitamin D3 and 10-DHGD intake induced significant hypoglycemic, hypolipidemic effects, decreased inflammation, and MI biomarkers. Decreased myocardial vacuoles, foam cells, and intimal lesions were also observed compared to DHC. Their combination intake induced more marked reduction in all biomarkers and showed a histopathological pattern similar to normal features of myocardium and aorta. Our findings suggest the therapeutic roles of vitamin D3, 10-DHGD, and their combination against myocardial injury in diabetic hyperlipidemic rats. Impact statement Hyperlipidemia represents a major risk factor for cardiovascular diseases leading to myocardial injury (MI). The present study aimed to illustrate the pattern of myocardial injury induced in diabetic hyperlipidemic rat model and the effect of vitamin D3, 10-dehydrogingerdione (10-DHGD) intake either individually or in combination form.

show abstract

Section: Methodsmentioning

confidence: 99%

Early myocardial injury biomarkers in diabetic hyperlipidemic rats: Impact of 10-dehydrogingerdione and vitamin D3

Elseweidy

Aly

Hammad

et al. 2020

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

show abstract

“…It had been assumed to be irreversible, and no agents were available to prevent it. However, a recent study reported that policosanol has the potential to inhibit vascular calcification [ 21 ]. Elseweidy et al reported that treating diabetic hyperlipidemic rats with policosanol, omega-3 fatty acids, and atorvastatin for eight weeks significantly increased high-density lipoprotein cholesterol (HDL-C) and vitamin D, decreased the number of aortic vacuoles, and inhibited the calcification process.…”

Section: Discussionmentioning

confidence: 99%

Aortic calcification burden predicts deterioration of renal function after radical nephrectomy

et al. 2017

View full text Add to dashboard Cite

BackgroundRadical nephrectomy for renal cell carcinoma (RCC) is a risk factor for the development of chronic kidney disease (CKD), and the possibility of postoperative deterioration of renal function must be considered before surgery. We investigated the contribution of the aortic calcification index (ACI) to the prediction of deterioration of renal function in patients undergoing radical nephrectomy.MethodsBetween January 1995 and December 2012, we performed 511 consecutive radical nephrectomies for patients with RCC. We retrospectively studied data from 109 patients who had regular postoperative follow-up of renal function for at least five years. The patients were divided into non-CKD and pre-CKD based on a preoperative estimated glomerular filtration rate (eGFR) of ≥60 mL/min/1.73 m2 or <60 mL/min/1.73 m2, respectively. The ACI was quantitatively measured by abdominal computed tomography before surgery. The patients in each group were stratified between low and high ACIs. Variables such as age, sex, comorbidities, and pre- and postoperative renal function were compared between patients with a low or high ACI in each group. Renal function deterioration-free interval rates were evaluated by Kaplan-Meier analysis. Factors independently associated with deterioration of renal function were determined using multivariate analysis.ResultsThe median age, preoperative eGFR, and ACI in this cohort were 65 years, 68 mL/min/1.73 m2, and 8.3%, respectively. Higher ACI (≥8.3%) was significantly associated with eGFR decline in both non-CKD and pre-CKD groups. Renal function deterioration-free interval rates were significantly lower in the ACI-high than ACI-low strata in both of the non-CKD and pre-CKD groups. Multivariate analysis showed that higher ACI was an independent risk factor for deterioration of renal function at 5 years after radical nephrectomy.ConclusionsAortic calcification burden is a potential predictor of deterioration of renal function after radical nephrectomy.Trial registrationThis study was registered as a clinical trial: UMIN000023577 Electronic supplementary materialThe online version of this article (doi:10.1186/s12894-017-0202-x) contains supplementary material, which is available to authorized users.

show abstract

“…The hypercholesterolemia diet (high-fat diet, HFD) consisted of the standard diet plus supplementation of the drinking water with a standardized suspension (CCT suspension) containing 4% cholesterol, 1% cholic acid and 0.5% 2-thiouracil. The diet was designed to induce atherogenic changes and hepatic steatosis as well as disturbances of lipid metabolism (hyperlipidemia), as suggested by similar studies [23][24][25][26].…”

Section: Dietsmentioning

confidence: 99%

Improvement in serum lipids and liver morphology after supplementation of the diet with fish oil is more evident under regular feeding conditions than under high-fat or mixed diets in rats

Godea¹,

Ciubotariu²,

Danciu³

et al. 2020

Lipids Health Dis

View full text Add to dashboard Cite

Background: Dietary n − 3 polyunsaturated fatty acids (PUFAs) have a role in preventing cardiovascular and hepatic diseases. However, their effects might differ significantly depending on individual dietary patterns. The aim of the present study was to evaluate the effects of dietary supplementation with ω-3 fatty acids (FA), administered in different schedules, on hepatic and aortic histological structure, lipid profile, and body weight (BW) in male Wistar rats under standard (SD), high-fat diet (HFD) and mixed feeding conditions. Methods: PUFA treatment consisted of the administration of 50 mg/kg fish oil (FO) daily by oral gavage. HFD was obtained by adding a suspension of 4% cholesterol, thiouracil and cholic acid to the animals' drinking water. The rats were maintained on the diets for 6 weeks, and different schedules of PUFA administration were used. At 14, 28, and 42 days, the morphology of liver and aortic samples and the levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), and triglycerides (TG) were assessed.

show abstract

Policosanol as a new inhibitor candidate for vascular calcification in diabetic hyperlipidemic rats

Cited by 24 publications

References 38 publications

Early myocardial injury biomarkers in diabetic hyperlipidemic rats: Impact of 10-dehydrogingerdione and vitamin D3

Early myocardial injury biomarkers in diabetic hyperlipidemic rats: Impact of 10-dehydrogingerdione and vitamin D3

Aortic calcification burden predicts deterioration of renal function after radical nephrectomy

Improvement in serum lipids and liver morphology after supplementation of the diet with fish oil is more evident under regular feeding conditions than under high-fat or mixed diets in rats

Contact Info

Product

Resources

About