Polarographic studies with the dropping mercury kathode. Part XIII. The effect of albumins

Heyrovský, J.; Babička, J.

doi:10.1135/cccc19300370

Cited by 82 publications

(46 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We reserve the denomination peak H only for the CPS peptide and protein signals. Signals obtained with polarography or voltammetry will be called presodium waves (or peaks) as earlier suggested by J. Heyrovsky et al [21]. In the presence of 70 mM urea this peak increased by about 20% (Fig.…”

Section: Linear Sweep and Elimination Voltammetry Of Ureadenatured Bsamentioning

confidence: 97%

Constant Current Chronopotentiometry and Voltammetry of Native and Denatured Serum Albumin at Mercury and Carbon Electrodes

et al. 2008

View full text Add to dashboard Cite

Constant current chronopotentiometric peak H at mercury electrodes was recently shown as a sensitive tool for global and local changes in protein conformation [1]. Large differences between the heights of peak H of native (hBSA nat ) and denatured BSA (hBSA den ) were observed. The ratio hBSA den /hBSA nat increased with more negative stripping current suggesting that the rate of potential change is important for discrimination between native and denatured BSA. Voltammetric peaks of BSA were less well developed and BSA den /BSA nat was much smaller. It was not possible to discriminate BSA den and BSA nat using carbon electrodes.

show abstract

Section: Linear Sweep and Elimination Voltammetry Of Ureadenatured Bsamentioning

confidence: 97%

Constant Current Chronopotentiometry and Voltammetry of Native and Denatured Serum Albumin at Mercury and Carbon Electrodes

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The history of the electrochemical analysis of proteins began in 1930 with the discovery of the so-called presodium polarographic wave by Heyrovský and Babička [1]. Shortly afterwards Brdička [2,3] demonstrated a double wave produced in ammoniacal cobalt solutions by proteins containing cystine or cysteine residues.…”

Section: Introductionmentioning

confidence: 99%

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

et al. 1998

View full text Add to dashboard Cite

Constant current derivative chronopotentiometric stripping analysis (CPSA) was used to study bioactive peptides [Lys 8 ]-vasopressin and angiotensin II at carbon paste electrode (CPE) and hanging mercury drop electrode (HMDE). Both peptides contain a single tyrosine residue which is oxidized at CPE close to þ0.6 V (against Ag/AgCl/3 M KCl electrode) producing peak Y; this peak was by about 15 mV more positive in vasopressin than in angiotensin II. At HMDE vasopressin yielded peak S due to the reduction of the disulfidic bond in the peptide. No peak S was observed with angiotensin II as it does not contain any cystine/cysteine residues. Vasopressin produced different catalytic hydrogen reduction signals in media with cobalt ion and in media not containing any metal ion. The signal produced in the latter media (peak H), which appeared close to ¹1.7 V, was well separated from the background. No catalytic hydrogen reduction signal was obtained with angiotensin II. The above mentioned CPSA signals can be applied for the determination of the respective peptide at nanomolar concentrations. The least sensitive peak S appears which requires at least 100 nM vasopressin concentration at moderate accumulation time. By two orders of magnitude, higher sensitivities can be obtained with peaks Y and H. In measuring these peaks CPSA shows significant advantages over the voltammetric stripping techniques.

show abstract

“…4 Their action was interpreted 5 as the mediating transfer of protons from solution to the electrode, where they combine with hydrogen atoms bound to the mercury surface under overpotential, and subsequently get reduced. Catalytic evolution of hydrogen from blood serum occurred at potentials more positive than the electroreduction of sodium ions, and thus that kind of hydrogen catalysis became known as »presodium catalysis«.…”

Section: »Presodium« Catalysismentioning

confidence: 99%

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Heyrovský

2006

ChemInform

View full text Add to dashboard Cite

show abstract

Polarographic studies with the dropping mercury kathode. Part XIII. The effect of albumins

Cited by 82 publications

References 0 publications

Constant Current Chronopotentiometry and Voltammetry of Native and Denatured Serum Albumin at Mercury and Carbon Electrodes

Constant Current Chronopotentiometry and Voltammetry of Native and Denatured Serum Albumin at Mercury and Carbon Electrodes

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Contact Info

Product

Resources

About