Abstract:samples of a constant concentration of urea nitrogen, and the fluorescence was measured. As seen in Figure 1, the fluorescence increased as the amount of sulfuric acid increased. The maximum fluorescence intensity occurred after the addition of 0.3 ml. Further addition caused a decrease in the intensity of fluorescence. This was the amount of acid selected for use in the procedure.The specificity of this reaction for urea has been discussed in previous publications (2,10,12). These authors show that contributi… Show more
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