Polarographic Assay Based on Hydrogen Peroxide Scavenging in Determination of Antioxidant Activity of Strong Alcohol Beverages

Gorjanović, Stanislava; Novaković, Miroslav; Vukosavljević, Predrag; Pastor, Ferenc T.; Tešević, Vele; Sužnjević, Desanka Ž.

doi:10.1021/jf101158j

Cited by 43 publications

(49 citation statements)

References 31 publications

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“…Higher correlation of HPS with ABTS (0.86, significant at p < 0.05 level for teas, and 0.85, significant at p < 0.01 level, for herbal infusions, at 95% confidence limit), than with DPPH scavenging (0.77, significant at p < 0.10 level, for teas, and 0.80, significant at p < 0.01 level, for herbal infusions, 95% confidence limit) is observed ( Table 2). Correlations between HPS and DPPH were high for wine samples (0.99) 23 and strong alcoholics (0.92), 24 whereas HPS has been correlated here with ABTS and FRAP for the first time. Finding that HPS correlates better with FC GAE than DPPH supports a previous conclusion that HPS reflects the sum of a wider range of AOs present than DPPH.…”

Section: ■ Results and Discussionmentioning

confidence: 68%

“…In our previous papers AO activity was expressed as volume required for 50% decrease of anodic oxidation current (AO 50 ), its reciprocal value (AO 50 −1 ), 22,23 or percentage of anodic oxidation current decrease obtained upon addition of 500 μL of samples (HPS500). 24 The slope has been introduced 21 as a more adequate way to express HPS activity, particularly when the activities of the analyzed samples vary significantly. 21 The results obtained by applying the novel electrochemical assay and commonly used spectrophotometric ones have been summarized in Table 1.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Until now, the assay has been successfully applied on various alcoholic beverages including beer, 22 red and white wines, 23 and various strong alcoholics. 24 In this paper the intention was to explore the feasibility of DC polarography in the determination of HPS activity of worldwide popular types of tea (Camellia sinesis) (white, yellow, green, oolong, and black), a tea-like beverage called maté(Ilex paraguariensis) that is widely consumed in Argentina, Brazil, Uruguay, and Paraguay and becoming increasingly popular in the United States and Europe, and herbal infusions used globally as traditional medicine and to prove the versatility of the assay in the analysis of various beverages, both alcoholic and nonalcoholic. This is the first report on DC polarographic assay application on nonalcoholic beverages.…”

Section: ■ Introductionmentioning

confidence: 99%

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Antioxidant Capacity of Teas and Herbal Infusions: Polarographic Assessment

Gorjanović

Komes

Pastor

et al. 2012

J. Agric. Food Chem.

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Hydrogen peroxide scavenging (HPS) activity of unfermented (green, yellow, and white), partially fermented (oolong), and completely fermented (black) tea ( Camellia sinensis ), maté ( Ilex paraguariensis ), and various herbal infusions, as well as individual compounds (flavan-3-ols, flavonols, cinnamic and benzoic acids, and methylxanthines), was assessed by recently developed direct current (DC) polarographic assay. Correlations of tea and herbal infusion HPS activity with total phenolic content determined using the Folin-Ciocalteu assay (FC-GAE) (0.81 and 0.93), ferric reducing/antioxidant power (FRAP) (0.97 and 0.92), 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.77 and 0.80), and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging (0.86 and 0.86) were statistically significant. Correlations between relative antioxidant capacity index (RACI), calculated by assigning all applied assays equal weight, and HPS (0.98), FRAP (0.97), ABTS (0.89), and DPPH (0.89) confirmed DC polarographic assay reliability when applied individually. Correlation analysis, ANOVA, and Levene and Tukey's HSD tests unequivocally confirmed this reliable, rapid, and low-cost assay validity, clearly demonstrating its advantages over spectrophotometric assays applied.

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Section: ■ Results and Discussionmentioning

confidence: 68%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant Capacity of Teas and Herbal Infusions: Polarographic Assessment

Gorjanović

Komes

Pastor

et al. 2012

J. Agric. Food Chem.

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“…Experiments were carried out according to the method previously described elsewhere with hydrolysate samples containing 2 mg cm −3 of protein (Jakovetić et al 2013a;Gorjanović et al 2010). DPPH antiradical scavenging activity, DPPH (%), was determined by using the following equation (Gorjanović et al 2010):…”

Section: Determination Of Antioxidant Activity As the Ability To Scavmentioning

confidence: 99%

Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit

Jakovetić

Luković

Jugović

et al. 2014

Food Bioprocess Technol

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The objective of this research was to design an efficient continuously operated membrane reactor with a separation unit for egg white protein (EWP) hydrolysis and production of hydrolysates with improved antioxidant properties. For this purpose, a mechanically stirred tank reactor coupled with the polyethersulfone ultrafiltration module with a molecular weight cutoff of 10 kDa was employed. Several proteolytic enzymes have been tested in order to obtain the best quality of peptide-based formulations intended for human consumption. Among protease from Bacillus licheniformis (Alcalase), protease from Bacillus amyloliquefaciens (Neutrase), and protease from papaya latex (papain), the highest degree of hydrolysis (DH), as well as the best antioxidant properties of obtained hydrolysates, was achieved with Alcalase. The effects of operating variables such as enzyme/ substrate ([E]/[S]) ratio, impeller speed, and permeate flow rate were further studied using response surface methodology (RSM) and Box-Behnken experimental design. Results obtained in RSM analysis confirmed that over the studied range [E]/[S] ratio, impeller speed and permeate flow rate had the significant effect on the DH and reactor capacity. The effects of different impeller geometries were also studied and fourbladed propeller stirrer enabled the highest DH. Antioxidant properties were analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), by the 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, and by the linear voltammetry methods. Results show that the use of Alcalase in the membrane reactor system is of potential interest for the EWP hydrolysis and obtaining value-added egg products.

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“…The polarographic oxidation of hydrogen peroxide on mercury electrodes has been used as a source of ROS to determine the antioxidant nature of phenolic compounds, wines, alcoholic drinks or beers [14][15][16][17][18]. The antioxidant capacity was determined from the decrease in the oxidation signal caused by the addition of antioxidant.…”

Section: Introductionmentioning

confidence: 99%