Polarizing activity in the developing limb of the Syrian hamster

Abstract

The transplantation of small pieces of tissue from the limb buds of 9 1/2 -10 day hamster embryos to the wing bud of the chick results in the induction of supernumerary wing structures. The anteroposterior polarity of these induced structures is under the control of the transplanted hamster tissue. The developing hamster limb thus has limb polarizing activity similar to that found in avian species and, as in the chick, the activity is found primarily in the posterior region of the limb bud.

Temporal pattern of posterior positional identity in mouse limb buds

“…Other authors have reported similar results with posterior cells from the limb buds of other mammals and from reptiles (MacCabe and Parker, 1976;Fallon and Crosby, 1977;Honig, 1984). We have made use of the ability of mouse posterior cells to elicit a supernumerary response after heterospecific grafting to the chick wing bud to map the temporal pattern of active posterior positional identity in the developing mouse limb bud.…”

“…Several previous studies have shown that cells from the posterior edge of limb buds of mice (Tickle et ak, 1976;Fallon and Crosby, 1977) as well as other vertebrates (MacCabe and Parker, 1976;Fallon and Crosby, 1977;Honig, 1984) are able to stimulate a supernumerary response when grafted into the anterior of a host chick wing bud. In this study we have used such a heterospecific assay to detect cells at different stages of mouse limb bud development with active posterior positional identity, as judged by their ability to stimulate a supernumerary response in chick limbs.…”

Control of the limb bud outgrowth in quail-chick chimera

“…The ability of the chick limb mesoderm to respond to ZPA grafts from many species is well documented. In such case, the chick limb responded within its own intrinsic abilities by making wing digits (MacCabe and Parker, 1976;Tickle et al, 1976;Fallon and Crosby, 1977). In our experiments, we focused on the growth of the limb and we analysed the size of the limb in detail.…”

Cellular contribution to supernumerary limbs resulting from the interaction between developing and regenerating tissues in the axolotl

“…Experimental evidence for this assumption stems primarily from the demonstration that a pattern regulatory response can be elicited from tissue grafts between different limbs within an organism (Saunders and Gasseling, 1968;Rollman-Dinsmore and Bryant, 1982), between limbs from evolutionarily diverse animals (Tickle et aZ., 1976;MacCabe and Parker, 1976;Fallon and Crosby, 1977), and between regenerating and developing limbs (Muneoka and Bryant, 1982). We have argued elsewhere for the validity of Wolperts' universality principle as it applies to the vertebrate limb (Muneoka and Bryant, 1984b).…”