Polarized two-photon fluorescence excitation spectra of indole and benzimidazole

Anderson, Bruce E.; Jones, Richard D.; Rehms, Aden A.; Ilich, Predrag; Callis, Patrik R.

doi:10.1016/0009-2614(86)85085-0

Cited by 41 publications

(15 citation statements)

References 24 publications

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“…3. 37 where I (2) is the measured emission intensity resulting from 2PE, C is the molar concentration, and (λ) is the differential luminescence quantum yield for the sample (A) and standard (B), respectively. The two-photon action cross-section, σ, a parameter widely used in 2PE spectroscopy, is defined as the product of the two-photon cross-section, δ, and the fluorescence quantum yield, : 36 The two-photon action cross-section of the sample, σ A , can be estimated using eqn.…”

Section: Two-photon Excitationmentioning

confidence: 99%

Multiphoton-excited luminescence of a lanthanide ion in a protein complex: Tb3+ bound to transferrin

White

Litvinenko

Meech

et al. 2004

Photochem Photobiol Sci

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The Tb(iii) complex of the iron-transport protein transferrin (Tb(2)-Tfr) exhibits strongly sensitised, sharp line luminescence from f-f states following multiphoton excitation via two tyrosinate residues directly co-ordinated to the lanthanide ion. Using an ultrafast Ti:sapphire laser system, a quadratic dependence of the Tb(iii) luminescence intensity was observed on excitation with photons at 503 and 566 nm, and a cubic dependence with photons at 800 nm. The two-photon cross-sections at 503 and 566 nm are 7.4 x 10(-50) and 0.37 x 10(-50) cm(4) s photon(-1) mol(-1), respectively, which compare favourably with values reported for the green fluorescent protein. Three-photon excitation at 800 nm gives rise to a Tb(iii) emission spectrum with excellent signal to noise ratios. These results lead to a proposal that if a Tb(iii)-protein complex with similar luminescent properties could be formed in vivo, an intra-cellular imaging system that uses multiphoton-excited, long-lived lanthanide ion luminescence could be developed. This offers the prospect of multiphoton imaging in tight focal planes using sharp line emission with long lifetimes for wavelength and time discrimination against background fluorescence.

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Section: Two-photon Excitationmentioning

confidence: 99%

Multiphoton-excited luminescence of a lanthanide ion in a protein complex: Tb3+ bound to transferrin

White

Litvinenko

Meech

et al. 2004

Photochem Photobiol Sci

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“…Secondly, we make use of the polarization properties of the excitation beams. Single-colour multi-photon techniques have been used to measure polarized two-photon spectra to elucidate the nature of the excited state of UV molecules in solution [16,17]. There are different selection rules for one-and two-photon spectroscopy; hence, one-photon 'forbidden states' can be characterized.…”

Section: Introductionmentioning

confidence: 99%

Applications of the time-resolved two-colour two-photon excitation of UV fluorophores using femtosecond laser pulses

et al. 2009

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A short overview of the principles and applications of the two-colour two-photon (2C2P) excitation of fluorescence by using femtosecond pulses is given. Fluorescence is generated by the simultaneous absorption of an 800 nm photon and a 400 nm photon of overlapping laser beams of a titanium:sapphire laser. Two examples of its application are presented: firstly, it is used to monitor the enzymatic cleavage of bovine serum albumin (BSA) by elastase. The fluorescent amino acid tryptophan present in BSA is excited corresponding to an effective one-photon wavelength of 266 nm. Secondly, it is shown how one can utilize the different polarizations of the excited beams for determining the symmetry of the excited states of molecules, exemplarily shown for p-terphenyl in cyclohexane. Further applications and experiments for 2C2P are suggested for using it in UV-fluorescence microscopy and for determining the properties of the electronic states of biomolecules by using differently polarized photons.

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“…Since then, two-photon spectroscopy has been used to study the excited state symmetry of organic chromophores [2][3][4]. Two-photon excitation has been applied to study hydrocarbons [5,6], porphyrins [7], polyenes [8,9], protein bound chromophores [10] and indole derivatives [11,12]. Two-photon excitation has also become a tool in analytical chemistry [13,14].…”

Section: Multi-photon Excitationmentioning

confidence: 99%

Advances in Fluorescence Spectroscopy: Multi-Photon Excitation, Engineered Proteins, Modulation Sensing and Microsecond Rhenium Metal-Ligand Complexes

et al. 1999

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The technology and applications of fluorescence spectroscopy are rapidly advancing. In this overview presentation we summarize some recent developments from this laboratory. Two and three-photon excitation have been observed for a wide variety of intrinsic and extrinsic fluorophores, including tryptophan, tyrosine, DNA stains, membrane probes, and even alkanes. It has been possible to observe multi-photon excitation of biopolymers without obvious photochemical or photo-thermal effects. Although not described in our lecture, another area of increasing interest is the use of engineered proteins for chemical and clinical sensing. We show results for the glucose-galactose binding protein from E. coli. The labeled protein shows spectral changes in response to micromolar concentrations of glucose. This protein was used with a novel sensing method based on the modulated emission of the labeled proteins and a long lifetime reference fluorophore. And finally, we describe a recently developed rhenium complex which displays a lifetime near 3 µs in oxygenated aqueous solution. Such long lifetime probes allow detection of microsecond dynamic processes, bypassing the usual nanosecond timescale limit of fluorescence. The result of these developments in protein engineering, sensing methods, and metal-ligand probe chemistry will be the increased use of fluorescence in clinical chemistry and point-of-care analyses.

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Polarized two-photon fluorescence excitation spectra of indole and benzimidazole

Cited by 41 publications

References 24 publications

Multiphoton-excited luminescence of a lanthanide ion in a protein complex: Tb3+ bound to transferrin

Multiphoton-excited luminescence of a lanthanide ion in a protein complex: Tb3+ bound to transferrin

Applications of the time-resolved two-colour two-photon excitation of UV fluorophores using femtosecond laser pulses

Advances in Fluorescence Spectroscopy: Multi-Photon Excitation, Engineered Proteins, Modulation Sensing and Microsecond Rhenium Metal-Ligand Complexes

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