Polarization of Steroid Fluorescence.

Strickler, Herbert S.; Grauer, Robert C.

doi:10.1021/ac60229a014

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Single Factor Experiments1

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2012

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“…Losing of the enzyme due to denaturation would reduce the reaction rate. Therefore, one of the most important factors facilitated the enzymolysis was that maintaining a proper temperature in the hydrolysis process [6][7][8], it was between the temperature of 37 ℃and 50 ℃. Fig.…”

Section: Single Factor Experimentsmentioning

confidence: 99%

Orthogonal Optimization of Enzymatic Hydrolysis of Whey Protein

Xia

2012

AMR

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Objective The study proposed an efficient and economic approach to hydrolyze whey protein isolate. Methods Using trypsin to hydrolyze the whey protein, this study investigated the influences of pH, hydrolysis temperature, enzyme-substrate ratio (E/S) and hydrolysis duration on the process of enzymatic hydrolysis. The orthogonal test method was used to design the experiments of the study. Result The optimized conditions of enzymatic hydrolysis were pH8, hydrolysis temperature 40°C，5% of E/S, and hydrolysis duration 2 hours. The hydrolysis degree was 26.5％. Conclusion The parameters identified from this study can benefit the development of whey protein.

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Section: Single Factor Experimentsmentioning

confidence: 99%