Polarization of IRON-REGULATED TRANSPORTER 1 (IRT1) to the plant-soil interface plays crucial role in metal homeostasis

Barberon, Marie; Dubeaux, Guillaume; Kolb, Cornelia; Isono, Erika; Zelazny, Enric; Vert, Grégory

doi:10.1073/pnas.1402262111

Cited by 224 publications

(262 citation statements)

References 56 publications

(70 reference statements)

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“…Although homologs of most ESCRT 0-III complex components occurring in yeast and mammals are found in the Arabidopsis genome (Barberon et al, 2014;Hurley and Emr, 2006), the functions of only a few of these homologs have been explored. Among these homologs, Arabidopsis FYVE1, which has been predicted to act as a subunit of ESCRT-I (a PI3P-binding protein that is located on late endosomes), is the most studied (Barberon et al, 2014;Hurley and Emr, 2006;Gao et al, 2014;Gao et al, 2015). FYVE1, also termed FREE1 (FYVE domain protein required for endosomal sorting 1), is essential for plant growth due to its role in MVB (Multivesicular Bodies) biogenesis and membrane-protein sorting in cells (Gao et al, 2014).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…FYVE1, also termed FREE1 (FYVE domain protein required for endosomal sorting 1), is essential for plant growth due to its role in MVB (Multivesicular Bodies) biogenesis and membrane-protein sorting in cells (Gao et al, 2014). FYVE1 acts as a crucial modulator of iron-regulated transporter1 (IRT1)-dependent metal transport and metal homeostasis by regulating the endosomal recycling of IRT1 back to the plasma membrane (Barberon et al, 2014). Additionally, a study of FREE1 implied the possibility of crosstalk between the ESCRT machinery and autophagy (Gao et al, 2015).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation

Lou

Tian

et al. 2016

Molecular Plant

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.All studies published in MOLECULAR PLANT are embargoed until 3PM ET of the day they are published as corrected proofs on-line. Studies cannot be publicized as accepted manuscripts or uncorrected proofs. proteasome system, which strengthens our understanding of both the turnover of ABA receptors and ESCRTs in plant hormone signaling. proteasome system, further strengthen our understanding of both the turnover of ABA receptors and ESCRTs in plant hormone signaling. M A N U S C R I P T A C C E P T E D

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation

Lou

Tian

et al. 2016

Molecular Plant

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“…(5). In addition, a recent study also demonstrated the function of FREE1/FYVE1 in regulating the polarization of iron-regulated transporter 1 (IRT1) to the plant-soil interface, and the IRT1-dependent metal homeostasis in plant (17). FREE1 is essential for plant growth and FREE1 depletion causes seedling lethality (Fig.…”

mentioning

confidence: 95%

Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation

Gao

Zhuang

Cui

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

175

166

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Protein turnover can be achieved via the lysosome/vacuole and the autophagic degradation pathways. Evidence has accumulated revealing that efficient autophagic degradation requires functional endosomal sorting complex required for transport (ESCRT) machinery. However, the interplay between the ESCRT machinery and the autophagy regulator remains unclear. Here, we show that FYVE domain protein required for endosomal sorting 1 (FREE1), a recently identified plant-specific ESCRT component essential for multivesicular body (MVB) biogenesis and plant growth, plays roles both in vacuolar protein transport and autophagic degradation. FREE1 also regulates vacuole biogenesis in both seeds and vegetative cells of Arabidopsis. Additionally, FREE1 interacts directly with a unique plant autophagy regulator SH3 DOMAIN-CONTAINING PROTEIN2 and associates with the PI3K complex, to regulate the autophagic degradation in plants. Thus, FREE1 plays multiple functional roles in vacuolar protein trafficking and organelle biogenesis as well as in autophagic degradation via a previously unidentified regulatory mechanism of cross-talk between the ESCRT machinery and autophagy process. T he endosomal-lysosomal/vacuolar pathway is the primary catabolic system of eukaryotic cells that degrades extracellular and intracellular materials. Membrane proteins destined for degradation, such as misfolded proteins or endocytosed receptors, become tagged by ubiquitin for further sorting to the endosomal-lysosomal/vacuolar system for degradation (1). During this process, an evolutionarily conserved machinery called endosomal sorting complex required for transport (ESCRT), is responsible for sorting these ubiquitinated cargos into the intraluminal vesicles (ILVs) of prevacuolar compartments/multivesicular bodies (PVCs/MVBs), which subsequently fuse with vacuoles/lysosomes to deliver their contents into the lumen for proteolytic degradation (2, 3). Malfunction of the assembly or dissociation of the ESCRT machinery disrupts MVB formation and thus results in the accumulation of ubiquitinated membrane cargos (4, 5).Macroautophagy (hereafter as autophagy) is another highly conserved catabolic process, which converges on the endosomallysosomal/vacuolar pathway to deliver aberrant organelles, longlived proteins, and protein aggregates to the lysosome/vacuole via a unique structure termed the "autophagosome" (6). Morphologically different from MVBs, autophagosomes are characterized by a double membrane structure, which is initiated from the phagophore assembly site/preautophagosome site (PAS) (7). The proteins or organelles to be degraded are encapsulated by autophagosomes that fuse either directly with the vacuole/lysosome or with endosomes like MVBs for expansion/maturation to form amphisomes, which then fuse with vacuole/lysosome for degradation. A number of conserved autophagy-related gene (ATG) proteins have been identified as participating in the autophagy pathway in eukaryotic cells (8).Even though it is generally accepted that at least one population of...

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“…The dominant genes responsible for these processes were first cloned from Arabidopsis (Arabidopsis thaliana), known as the ferric-chelate reductase oxidase gene FRO2 and the Fe-regulated transporter gene IRT1 (Eide et al, 1996;Robinson et al, 1999). The expression of both genes is induced by Fe deficiency and is tightly regulated at multiple levels (Connolly et al, 2002(Connolly et al, , 2003Barberon et al, 2011Barberon et al, , 2014Shin et al, 2013;Ivanov et al, 2014).…”

mentioning

confidence: 99%

A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency

Yan

Li²,

Sun³

et al. 2016

Plant Physiol.

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Iron (Fe) deficiency affects plant growth and development, leading to reduction of crop yields and quality. Although the regulation of Fe uptake under Fe deficiency has been well studied in the past decade, the regulatory mechanism of Fe translocation inside the plants remains unknown. Here, we show that a WRKY transcription factor WRKY46 is involved in response to Fe deficiency. Lack of WRKY46 (wrky46-1 and wrky46-2 loss-of-function mutants) significantly affects Fe translocation from root to shoot and thus causes obvious chlorosis on the new leaves under Fe deficiency. Gene expression analysis reveals that expression of a nodulin-like gene (VACUOLAR IRON TRANSPORTER1-LIKE1 [VITL1]) is dramatically increased in wrky46-1 mutant. VITL1 expression is inhibited by Fe deficiency, while the expression of WRKY46 is induced in the root stele. Moreover, down-regulation of VITL1 expression can restore the chlorosis phenotype on wrky46-1 under Fe deficiency. Further yeast one-hybrid and chromatin immunoprecipitation experiments indicate that WRKY46 is capable of binding to the specific W-boxes present in the VITL1 promoter. In summary, our results demonstrate that WRKY46 plays an important role in the control of root-to-shoot Fe translocation under Fe deficiency condition via direct regulation of VITL1 transcript levels.

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Polarization of IRON-REGULATED TRANSPORTER 1 (IRT1) to the plant-soil interface plays crucial role in metal homeostasis

Cited by 224 publications

References 56 publications

ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation

ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation

Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation

A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency

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