Polarization interference mapping of microscopic images of protein fluorofores in the differential diagnosis of benign and malignant prostate tumours

Trifonyuk, L.; Strashkevich, A.M.; Pavlyukovich, N.; Pavlyukovich, A; Tomka, Yu. Ya.; Zhytaryuk, V. G.; Tkachuk, V. I.

doi:10.1117/12.2616836

Cited by 1 publication

(9 citation statements)

References 6 publications

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“…3. Analytical processing of microscopic interference images of dehydrated film samples was carried out using the digital Fourier transform

[ 49 ]:

where

, orthogonal components of complex amplitude for different orientations,

;

denotes the complex conjugation operation;

are the spatial frequencies and

are the number of pixels of the CCD camera…”

Section: The Methods Of 3d Jones Matrix Scanning Of Bpf and Sfmentioning

confidence: 99%

“…3. Analytical processing of microscopic interference images of dehydrated film samples was carried out using the digital Fourier transform 𝐹𝐹(𝜐𝜐, 𝜈𝜈) [49]:…”

Section: The Methods Of 3d Jones Matrix Scanning Of Bpf and Sfmentioning

confidence: 99%

“…The resulting set of optical anisotropy maps

was analyzed in a statistical approach using the following algorithms to calculate mean (

), variance (

), skewness (

) and kurtosis (

) [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ]

where

—CCD pixels quantity.…”

Section: Statistical Analysis Of Maps Of Linear and Circular Birefrin...mentioning

confidence: 99%

“…Polarimetric studies of the polarization manifestations of BF anisotropy make it possible to detect pathological changes in the quaternary and tertiary polycrystalline structure of basic proteins (albumin and globulin) in blood plasma and other biological fluids, which are inaccessible in the most common biochemical methods. In a series of works [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ], the sensitivity of polarimetric methods to inflammatory, pathological and necrotic changes in the polycrystalline structure of films of blood plasma, urine, synovial fluid, and cerebrospinal fluid was demonstrated.…”

Section: Introductionmentioning

confidence: 99%

“…This polarimetric approach can be developed and generalized into a synthesis of polarization interference mapping and digital holographic reconstruction of object fields of complex amplitudes for layer-by-layer reconstruction of optical anisotropy maps of dehydrated BF films. Therefore, to improve the sensitivity and accuracy of polarimetric matrix diagnostics, it is urgent to create a new method for the layer-by-layer study of polycrystalline films of biological fluids by synthesizing polarization (Jones matrix) and interference methods [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

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Optical Sensor System for 3D Jones Matrix Reconstruction of Optical Anisotropy Maps of Self-Assembled Polycrystalline Soft Matter Films

Wójcik,

Hu,

Ushenko

et al. 2024

Sensors

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Our work uses a polarization matrix formalism to analyze and algorithmically represent optical anisotropy by open dehydration of blood plasma films. Analytical relations for Jones matrix reconstruction of optical birefringence maps of protein crystal networks of dehydrated biofluid films are found. A technique for 3D step-by-step measurement of the distributions of the elements of the Jones matrix or Jones matrix images (JMI) of the optically birefringent structure of blood plasma films (BPF) has been created. Correlation between JMI maps and corresponding birefringence images of dehydrated BPF and saliva films (SF) obtained from donors and prostate cancer patients was determined. Within the framework of statistical analysis of layer-by-layer optical birefringence maps, the parameters most sensitive to pathological changes in the structure of dehydrated films were found to be the central statistical moments of the 1st to 4th orders. We physically substantiated and experimentally determined the sensitivity of the method of 3D polarization scanning technique of BPF and SF preparations in the diagnosis of endometriosis of uterine tissue.

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“…3. Analytical processing of microscopic interference images of dehydrated film samples was carried out using the digital Fourier transform

[ 49 ]:

where

, orthogonal components of complex amplitude for different orientations,

;

denotes the complex conjugation operation;

are the spatial frequencies and

are the number of pixels of the CCD camera…”

Section: The Methods Of 3d Jones Matrix Scanning Of Bpf and Sfmentioning

confidence: 99%

“…3. Analytical processing of microscopic interference images of dehydrated film samples was carried out using the digital Fourier transform 𝐹𝐹(𝜐𝜐, 𝜈𝜈) [49]:…”

Section: The Methods Of 3d Jones Matrix Scanning Of Bpf and Sfmentioning

confidence: 99%

“…The resulting set of optical anisotropy maps

was analyzed in a statistical approach using the following algorithms to calculate mean (

), variance (

), skewness (

) and kurtosis (

) [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ]

where

—CCD pixels quantity.…”

Section: Statistical Analysis Of Maps Of Linear and Circular Birefrin...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optical Sensor System for 3D Jones Matrix Reconstruction of Optical Anisotropy Maps of Self-Assembled Polycrystalline Soft Matter Films

Wójcik,

Hu,

Ushenko

et al. 2024

Sensors

View full text Add to dashboard Cite

show abstract

Polarization interference mapping of microscopic images of protein fluorofores in the differential diagnosis of benign and malignant prostate tumours

Cited by 1 publication

References 6 publications

Optical Sensor System for 3D Jones Matrix Reconstruction of Optical Anisotropy Maps of Self-Assembled Polycrystalline Soft Matter Films

Optical Sensor System for 3D Jones Matrix Reconstruction of Optical Anisotropy Maps of Self-Assembled Polycrystalline Soft Matter Films

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