Point of Care Sensors for Infectious Pathogens

Sharafeldin, Mohamed; Davis, Jason J.

doi:10.1021/acs.analchem.0c04677

Cited by 66 publications

(60 citation statements)

References 166 publications

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“…Other than clinical diagnostic techniques, point-of-care (POC) diagnostic systems could pave the way and start a new era for the screening of viruses. 20 …”

Section: Introductionmentioning

confidence: 99%

Rapid Point-of-Care COVID-19 Diagnosis with a Gold-Nanoarchitecture-Assisted Laser-Scribed Graphene Biosensor

et al. 2021

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The global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has revealed the urgent need for accurate, rapid, and affordable diagnostic tests for epidemic understanding and management by monitoring the population worldwide. Though current diagnostic methods including real-time polymerase chain reaction (RT-PCR) provide sensitive detection of SARS-CoV-2, they require relatively long processing time, equipped laboratory facilities, and highly skilled personnel. Laser-scribed graphene (LSG)-based biosensing platforms have gained enormous attention as miniaturized electrochemical systems, holding an enormous potential as point-of-care (POC) diagnostic tools. We describe here a miniaturized LSG-based electrochemical sensing scheme for coronavirus disease 2019 (COVID-19) diagnosis combined with three-dimensional (3D) gold nanostructures. This electrode was modified with the SARS-CoV-2 spike protein antibody following the proper surface modifications proved by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) characterizations as well as electrochemical techniques. The system was integrated into a handheld POC detection system operated using a custom smartphone application, providing a user-friendly diagnostic platform due to its ease of operation, accessibility, and systematic data management. The analytical features of the electrochemical immunoassay were evaluated using the standard solution of S-protein in the range of 5.0–500 ng/mL with a detection limit of 2.9 ng/mL. A clinical study was carried out on 23 patient blood serum samples with successful COVID-19 diagnosis, compared to the commercial RT-PCR, antibody blood test, and enzyme-linked immunosorbent assay (ELISA) IgG and IgA test results. Our test provides faster results compared to commercial diagnostic tools and offers a promising alternative solution for next-generation POC applications.

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“…Other than clinical diagnostic techniques, point-of-care (POC) diagnostic systems could pave the way and start a new era for the screening of viruses. 20 …”

Section: Introductionmentioning

confidence: 99%

Rapid Point-of-Care COVID-19 Diagnosis with a Gold-Nanoarchitecture-Assisted Laser-Scribed Graphene Biosensor

et al. 2021

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“…Bioreceptors are immobilized on the gate terminal. The terminal is connected to both the source and drain electrodes [152][153][154][155]. The sensor's performance is tested using an antigen biomarker obtained from nasopharyngeal swab specimens of infected COVID-19 patients.…”

Section: Graphene Field Effect Transistormentioning

confidence: 99%

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

et al. 2021

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COVID-19, also known as SARS-CoV-2 is a novel, respiratory virus currently plaguing humanity. Genetically, at its core, it is a single-strand positive-sense RNA virus. It is a beta-type Coronavirus and is distinct in its structure and binding mechanism compared to other types of coronaviruses. Testing for the virus remains a challenge due to the small market available for at-home detection. Currently, there are three main types of tests for biomarker detection: viral, antigen and antibody. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) remains the gold standard for viral testing. However, the lack of quantitative detection and turnaround time for results are drawbacks. This manuscript focuses on recent advances in COVID-19 detection that have lower limits of detection and faster response times than RT-PCR testing. The advancements in sensing platforms have amplified the detection levels and provided real-time results for SARS-CoV-2 spike protein detection with limits as low as 1 fg/mL in the Graphene Field Effect Transistor (FET) sensor. Additionally, using multiple biomarkers, detection levels can achieve a specificity and sensitivity level comparable to that of PCR testing. Proper biomarker selection coupled with nano sensing detection platforms are key in the widespread use of Point of Care (POC) diagnosis in COVID-19 detection.

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“…Facing an unprecedented pandemic threat, fast and sensitive molecular diagnostic tests play an important role in controlling the spread of infectious pathogens and enable early disease management. In molecular diagnosis, nucleic acid amplification tests (NAATs) deliver highly sensitive detection of extremely low-concentrated pathogens in the early stage of diseases, especially newly emerging and re-emerging infectious outbreaks ( Innis et al, 1988 ; Sharafeldin and Davis, 2021 ; Yang and Rothman, 2004 ). Recently, COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has grown into a global pandemic and has become a serious health issue ( Kevadiya et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

Das

Lin

Kwon

et al. 2022

Biosensors and Bioelectronics

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Point of Care Sensors for Infectious Pathogens

Abstract: profoundly improved epidemiological control, food safety, and general biodefense. The potential socio-economic impact of getting this right is profound.

Cited by 66 publications

References 166 publications

Rapid Point-of-Care COVID-19 Diagnosis with a Gold-Nanoarchitecture-Assisted Laser-Scribed Graphene Biosensor

Rapid Point-of-Care COVID-19 Diagnosis with a Gold-Nanoarchitecture-Assisted Laser-Scribed Graphene Biosensor

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA

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