Point‐of‐care method for total white cell count: an evaluation of the HemoCue WBC device

Abstract

Point-of-care testing (POCT) is becoming an important adjunct to haematology laboratory practice. An important component of the blood count is the total white cell count (WBC). Previously, this required laborious microscopic cell counting, but it can now be performed by means of automation; however, in many under-resourced countries, costly automated counters are only available in very few central hospitals. Moreover, neither method is practical in most POCT situations. The HemoCue WBC has been developed as a simplified alternative method, consisting of a reagent pre-loaded disposable cuvette together with basic image analysis technology. This report describes an assessment of its utility. The WBC of 500 routine blood samples from the hospital were tested in parallel by the HemoCue WBC and by a reference analyser to assess accuracy and utility of the former. The tests included precision, linearity, type of blood sample and anticoagulant and potential interfering substances in blood specimens. In the tests for accuracy, 192 of the 200 showed percentage difference from the NEQAS reference of <10% whilst the remaining eight samples differed by <12%, thus meeting the requirements of Clinical laboratory improvement amendments (CLIA)-88 regulations. Of the samples tested with potential interfering substances only those with >2% normoblasts or reticulocytosis showed significant differences from the reference measurements. The HemoCue WBC is reliable for WBC counts within the analytical range of 0.4–30.0 × 109/l, except in samples where there are significant numbers of normoblasts or reticulocytes. It is simple to use and provides a valuable advance in the facilities available for POCT in haematology.

Lipid Profili, Serum Ürik Asit, Glukoz ve İnsülin Direnci Üzerine Gilbert"s Sendromunun Etkileri

“…Corrected reticulocyte counts were done with a reticulocyte smear to the patients with normal levels of LDH, and the levels of more than 2% were excluded 14 .…”

Automated end-to-end blood testing at the point-of-care: Integration of robotic phlebotomy with downstream sample processing

“…For example, handheld analyzers such as the iSTAT device 12,14 use disposable cartridges to assess basic chemistry, blood gases, and protein markers, primarily using capillary blood from the finger. Automated cell counters such as the Chempaq XBC 15 and HemoCue WBC 16 have also been introduced to perform cell counts from capillary blood. However, the main limitation with POCT analyzers is the reliance on capillary blood from finger-pricks.…”

The effect of Gilbert’s syndrome on the dispersions of QT and P-wave dispersion: an observational study

“…Increased levels of IB with elevated levels of LDH were considered as hemolysis and excluded from the study (18). Corrected reticulocyte counts were done with a reticulocyte smear to the patients with normal levels of LDH, and the levels of more than 2% were excluded (19). The study was approved by the local ethics committees, and informed consent from each participant was obtained (Approval No: 2012/114).…”