Podocan Is Expressed in Blood and Adipose Tissue and Correlates Negatively With the Induction of Diabetic Nephropathy

Nio, Yasunori; Okawara, Mitsugi; Okuda, Suguru; Matsuo, Taisuke; Furuyama, Naoki

doi:10.1210/js.2017-00123

Cited by 8 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PODN (Podocan) is highly homologous to members of the small leucine-rich repeat protein (SLRP) family, which are proteoglycans that regulate and maintain extracellular matrix collagen fibrils. It is highly expressed in adipocytes ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Adipose Tissue Properties in Tumor-Bearing Breasts

et al. 2020

View full text Add to dashboard Cite

The tissue stroma plays a major role in tumors' natural history. Most programs for tumor progression are not activated as cell-autonomous processes but under the conditions of cross-talks between tumor and stroma. Adipose tissue is a major component of breast stroma. This study compares adipose tissues in tumor-bearing breasts to those in tumor-free breasts with the intention of defining a signature that could translate into markers of cancer risk. In tumor-bearing breasts, we sampled adipose tissues adjacent to, or distant from the tumor. Parameters studied included: adipocytes size and density, immune cell infiltration, vascularization, secretome and gene expression. Adipose tissues from tumor-bearing breasts, whether adjacent to or distant from the tumor, do not differ from each other by any of these parameters. By contrast, adipose tissues from tumor-bearing breasts have the capacity to secrete twice as much interleukin 8 (IL-8) than those from tumor-free breasts and differentially express a set of 137 genes of which a significant fraction belongs to inflammation, integrin and wnt signaling pathways. These observations show that adipose tissues from tumor-bearing breasts have a distinct physiological status from those from tumor-free breasts. We propose that this constitutive status contributes as a non-cell autonomous process to determine permissiveness for tumor growth.

show abstract

Section: Discussionmentioning

confidence: 99%

Adipose Tissue Properties in Tumor-Bearing Breasts

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Podocan belongs to the fifth class of the SLR protein family and is expressed in the sclerotic glomerular lesions of experimental human immunodeficiency virus-associated nephropathy (HIVAN) (Ross et al, 2003; Shimizu-Hirota et al, 2004b). Podocan is involved in kidney function and may represent a unique therapeutic target for the treatment of diabetic nephropathy (Nio et al, 2017). Moreover, a lack of podocan results in excessive arterial repair and prolonged smooth muscle cell (SMC) proliferation, which is likely mediated by the Wnt/β-catenin pathway (Hutter et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Podocan Promotes Differentiation of Bovine Skeletal Muscle Satellite Cells by Regulating the Wnt4-β-Catenin Signaling Pathway

Liú

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

Background Small leucine-rich repeat proteins (SLRPs) are highly effective and selective modulators of cell proliferation and differentiation. Podocan is a newly discovered member of the SLRP family. Its potential roles in the differentiation of bovine muscle-derived satellite cells (MDSCs) and its underlying functional mechanism remain unclear. Our study aimed to characterize the function of the podocan gene in the differentiation of bovine MDSCs and to clarify the molecular mechanism by which podocan functions in order to contribute to a better understanding of the molecular mechanism by which extracellular matrix promotes bovine MDSC differentiation and provide a theoretical basis for the improvement of beef quality. Methods Bovine MDSCs were transfected with vectors to overexpress or inhibit podocan, and podocan protein was added to differentiation culture medium. qRT-PCR, western blotting, and immunofluorescence were performed to investigate the effects of podocan on MDSC differentiation. Confocal microscopy and western blotting were used to assess the nuclear translocation and expression of β-catenin. An inhibitor and activator of β-catenin were used to assess the effects of the Wnt/β-catenin signaling pathway on MDSC differentiation. We inhibited β-catenin while overexpressing podocan in MDSCs. Then, we performed mass spectrometry to identify which proteins interact with podocan to regulate the Wnt/β-catenin signaling pathway. Finally, we confirmed the relationship between podocan and Wnt4 by co-immunoprecipitation and western blotting. Results Podocan protein expression increased significantly during bovine MDSC differentiation. Differentiation of bovine MDSC was promoted and suppressed by podocan overexpression or inhibition, respectively. Podocan was also shown to modulate the Wnt/β-catenin signaling pathway. Treatment of bovine MDSCs with β-catenin inhibitor and activator showed that the Wnt/β-catenin pathway is involved in bovine MDSC differentiation. Furthermore, the effect of podocan on bovine MDSC differentiation was suppressed when this pathway was inhibited. We also found that podocan interacts with Wnt4. When Wnt4 was inhibited, podocan-induced promotion of bovine MDSC differentiation was attenuated through Wnt/β-catenin signaling. Conclusion Podocan regulates Wnt/β-catenin through Wnt4 to promote bovine MDSC differentiation.

show abstract

“…TBX2 was involved in the progression of hypertension [163], but this gene may be associated with development of CAD. Genes such as podocan (PODN) [164] and PAS domain containing serine/threonine kinase (PASK) [165] were liable for progression of diabetes, but these genes may be linked with progression of CAD. In the target gene -miRNA regulatory network, 5 up regulated genes and 5 down regulated genes with a high node degree was chosen as target gene.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Signatures Reveal Candidate Key Genes in the Peripheral Blood Mononuclear Cells of Patients With Coronary Artery Disease

Kolur¹,

Vastrad²,

Vastrad³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundCoronary artery disease (CAD) is one of the most common disorders in the cardiovascular system. This study aims to explore potential signaling pathways and important biomarkers that drive CAD development. MethodsThe CAD GEO Dataset GSE113079 was featured to screen differentially expressed genes (DEGs). The pathway and Gene Ontology (GO) enrichment analysis of DEGs were analyzed using the ToppGene. We screened hub and target genes from protein-protein interaction (PPI) networks, target gene - miRNA regulatory network and target gene - TF regulatory network, and Cytoscape software. Validations of hub genes were performed to evaluate their potential prognostic and diagnostic value for CAD. Results1,036 DEGs were captured according to screening criteria (525upregulated genes and 511downregulated genes). Pathway and Gene Ontology (GO) enrichment analysis of DEGs revealed that these up and down regulated genes are mainly enriched in thyronamine and iodothyronamine metabolism, cytokine-cytokine receptor interaction, nervous system process, cell cycle and nuclear membrane. Hub genes were validated to find out potential prognostic biomarkers, diagnostic biomarkers and novel therapeutic target for CAD. ConclusionsIn summary, our findings discovered pivotal gene expression signatures and signaling pathways in the progression of CAD. CAPN13, ACTBL2, ERBB3, GATA4, GNB4, NOTCH2, EXOSC10, RNF2, PSMA1 and PRKAA1 might contribute to the progression of CAD, which could have potential as biomarkers or therapeutic targets for CAD.

show abstract

Podocan Is Expressed in Blood and Adipose Tissue and Correlates Negatively With the Induction of Diabetic Nephropathy

Cited by 8 publications

References 37 publications

Adipose Tissue Properties in Tumor-Bearing Breasts

Adipose Tissue Properties in Tumor-Bearing Breasts

Podocan Promotes Differentiation of Bovine Skeletal Muscle Satellite Cells by Regulating the Wnt4-β-Catenin Signaling Pathway

Transcriptome Signatures Reveal Candidate Key Genes in the Peripheral Blood Mononuclear Cells of Patients With Coronary Artery Disease

Contact Info

Product

Resources

About