PO-86 Integrin alpha(v)beta(3) and osteopontin promote hematogenous metastasis of osteosarcoma cells to the lung

Hassenpflug, Wolf; Tschan, Mario P.; Torbett, Bruce E.; Felding-Habermann, B.

doi:10.1016/s0049-3848(07)70239-6

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“…The selective cytotoxicity of curcumin-loaded APNPs was also greater than that of curcumin dissolved in the organic solvent DMSO, which may be because the RGD sequence targets overexpressed integrins on cancerous cells. 34,[40][41][42] Since DMSO has been shown to enhance the penetration of both hydrophobic and hydrophilic agents into the cell membrane, 43 curcumin could be delivered through the lipid membrane with the aid of DMSO. By becoming incorporated below the hydrophilic head group in a nonspecific manner, molecules of DMSO increase the average head group area and the amenability to bending in the lipid membranes, the entry of water molecules is then facilitated, inducing non-specific water pore formation.…”

Section: Discussionmentioning

confidence: 99%

Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Chang¹,

Sun²,

Webster³

2015

IJN

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Osteosarcoma is the most frequent primary malignant form of bone cancer, comprising 30% of all bone cancer cases. The objective of this in vitro study was to develop a treatment against osteosarcoma with higher selectivity toward osteosarcoma cells and lower cytotoxicity toward normal healthy osteoblast cells. Curcumin (or diferuloylmethane) has been found to have antioxidant and anticancer effects by multiple cellular pathways. However, it has lower water solubility and a higher degradation rate in alkaline conditions. In this study, the amphiphilic peptide C18GR7RGDS was used as a curcumin carrier in aqueous solution. This peptide contains a hydrophobic aliphatic tail group leading to their self-assembly by hydrophobic interactions, as well as a hydrophilic head group composed of an arginine-rich and an arginine-glycine-aspartic acid structure. Through characterization by transmission electron microscopy, self-assembled structures of spherical amphiphilic nanoparticles (APNPs) with diameters of 10–20 nm in water and phosphate-buffered saline were observed, but this structure dissociated when the pH value was reduced to 4. Using a method of codissolution with acetic acid and dialysis tubing, the solubility of curcumin was enhanced and a homogeneous solution was formed in the presence of APNPs. Successful encapsulation of curcumin in APNPs was then confirmed by Fourier transform infrared and X-ray diffraction analyses. The cytotoxicity and cellular uptake of the APNP/curcumin complexes on both osteosarcoma and normal osteoblast cell lines were also evaluated by methyl-thiazolyl-tetrazolium assays and confocal fluorescence microscopy. The results showed that the curcumin-loaded APNPs had significant selective cytotoxicity against MG-63 osteosarcoma cells when compared with normal osteoblasts. We have demonstrated for the first time that APNPs can encapsulate hydrophobic curcumin in their hydrophobic cores, and curcumin-loaded APNPs could be an innovative treatment for the selective inhibition of osteosarcoma cells.

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Section: Discussionmentioning

confidence: 99%

Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Chang¹,

Sun²,

Webster³

2015

IJN

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PO-86 Integrin alpha(v)beta(3) and osteopontin promote hematogenous metastasis of osteosarcoma cells to the lung

Cited by 1 publication

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Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

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PO-86 Integrin alpha(v)beta(3) and osteopontin promote hematogenous metastasis of osteosarcoma cells to the lung

Cited by 1 publication

References 0 publications

Selective inhibition of MG-63 osteosarcoma cell&nbsp;proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Selective inhibition of MG-63 osteosarcoma cell&nbsp;proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

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Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Selective inhibition of MG-63 osteosarcoma cell proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres