PO-22 - Thrombodynamics test for analysis of hemostasis in patients with malignant tumors and predicting thrombotic complications

Василенко, И. А.; Vlasova, Elizaveta; Метелин, В. Б.; Балканов, А. С.; Bichenkov, O.

doi:10.1016/s0049-3848(16)30155-4

Cited by 3 publications

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“…The assay sensitivity to pre-analytical conditions and method error when different protocols was used is described in [34]. Thrombodynamics test turned to be sensitive to presence of active factors VIIa, IXa, XIa [35,36], microparticles [21,36], unfractionated heparin and enoxaparin [37] in plasma, impaired coagulation in hemophilia [38], hypercoagulation in patients with sepsis [39], malignant tumors [40], multiple myeloma [41]. The assay was shown to be useful for monitoring heparin efficiency [37,42], predicting negative in vitro fertilization caused by hypercoagulation [43].…”

Section: Thrombodynamics Testmentioning

confidence: 99%

Use of Thrombodynamics for revealing the participation of platelet, erythrocyte, endothelial, and monocyte microparticles in coagulation activation and propagation

et al. 2020

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Background and objectiveFor many pathological states, microparticles are supposed to be one of the causes of hypercoagulation. Although there are some indirect data about microparticles participation in coagulation activation and propagation, the integral hemostasis test Thrombodynamics allows to measure micropaticles participation in these two coagulation phases directly. Demonstrates microparticles participation in coagulation activation by influence on the appearance of coagulation centres in the plasma volume and the rate of clot growth from the surface with immobilized tissue factor.Methods: Microparticles were obtained from platelets and erythrocytes by stimulation with thrombin receptor-activating peptide (SFLLRN) and calcium ionophore (A23187), respectively, from monocytes, endothelial HUVEC culture and monocytic THP cell culture by stimulation with lipopolysaccharides. Microparticles were counted by flow cytometry and titrated in microparticle-depleted normal plasma in the Thrombodynamics test. ResultsMonocyte microparticles induced the appearance of clotting centres through the TF pathway at concentrations approximately 100-fold lower than platelet and erythrocyte microparticles, which activated plasma by the contact pathway. For endothelial microparticles, both activation pathways were essential, and their activity was intermediate. Monocyte microparticles induced plasma clotting by the appearance of hundreds of clots with an extremely slow growth rate, while erythrocyte microparticles induced the appearance of a few clots with a growth rate similar to that from surface covered with high-density tissue factor. Patterns of clotting induced by platelet and endothelial microparticles were intermediate.

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