PO-0927 The Difference Spectrum Of Activity Of Synthetic Interferon Inductors

Abstract

ethnographic approach was taken; the consensus meetings were overseen by a facilitator and non-participant observations of the meetings were recorded. Post consensus meeting semi-structured interviews were conducted with the group members. Qualitative and quantitative analyses were carried out. We examined the extent to which individuals and groups agreed with the 'gold standard' using percentage exact agreement (%EA) (Figure 1). Results Agreement ranged from 35-70%. The mean agreement for individuals was 54% and 47% for the consensus groups. Conclusion In assessing avoidability of ADRs individual assessments had better agreement with the 'gold standard' evaluation than group assessments. Qualitative analysis of meeting observations and participant interviews may help identify reasons for this and inform the optimisation of the LAAT for assessment of ADR avoidability. Objectives Until now the stimulating effects of these interferonogens have been shown for type I and II IFNs, however the data regarding induction of type III IFNs has not been available. Methods The IFN (a, b, g and 2/3) inducing activities of low-molecular synthetic interferonogens tilorone (amixin), meglumine acridonacetate (cycloferon), sodium carboxymethylcellulose and gossypol conjugate (kagocel) are compared in CBA mice. Results PO-0927Administration of tilorone in comparison to cycloferon and kagocel has a significant dose-dependent effect on IFNs concentration in mice serum. Maximal level of all IFNs in serum was observed after 24 h of administration of tilorone (IFNa 176 ± 10 and 398 ± 12 pg/ml for the doses of 40 and 400 mg/kg, IFNb 8 ± 4-110 ± 6, IFNg 4 ± 1-46 ± 10, IFN2/3 1337 ± 93 and 2231 ± 93 pg/ml). Cycloferon and kagocel do not induce the increase of IFNa, IFNb and IFNg in mice serum.The highest level of IFN2/3 after introduction of kagocel at the both doses was seen after 48 h (616 ± 135-382 ± 46 pg/ml). Administration of cycloferon at a dose of 150 mg/kg significantly stimulated IFN2/3 level in serum after 72-120 h of administration (391 ± 31-388 ± 80 pg/ml). Cycloferon at the highest dose significantly increased the IFN2/3 levels between 12-24 h after administration (434 ± 79-441 ± 47 pg/ml). Conclusion Tilorone induces synthesis of type I and type II IFNs as well as type III IFNs (IFN2/3) in serum, while administration of kagocel and cycloferon stimulates only type III IFNs (IFN2/3) in serum. Tilorone (amixin) has the widest spectrum of activity and is the most promising of the studied interferon inducers. Background and aims Rotavirus accounts for more than 50% of gastroenteritis worldwide and leads to significant morbidity and mortality especially among children younger than five. The drug utilisation profile in rotavirus infection among the admitted children < 5 years with gastroenteritis was studied. Methods Cross-sectional hospital based study was carried out among children age <5 years admitted to Paediatric ward with Rotavirus gasteroenteritis (RVGE) from 2011-12. A data collection form (proforma) was used to record the...