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Abstract

A simple, precise, accurate, and rapid high performance thin layer chromatographic method has been developed and completely validated for the estimation of hydroxychavicol isolated from piper betel leaves powder. The isolation of hydroxychavicol was carried out using flash chromatography. Validation was carried out with pre-coated silica gel aluminum plate G 60 F254 (10 x 10 cm) as the stationary phase, mobile phase consisting of Butanol: Ethyl Acetate in the composition of 7:3 (v/v) and scanned in Absorbance Reflectance mode at 280 nm using Camag TLC scanner 3 with WinCAT software. The Rf value of 0.64 was obtained for isolated hydroxychavicol. The proposed method was validated over a linearity range of 200-800 ng/spot, and its percentage recovery was calculated along with intraday and interday precision. The limit of detection and the limit of quantification were found to be 13.890 ng/spot and 40.457 ng/spot, respectively. The suggested method can be successfully used to detect and quantify hydroxychavicol in herbal formulations.