Pneumoslide-M Technique for Rapid Detection of Atypical Pathogens in Critically ILL Children with Lower Respiratory Tract Infections

El-Sahrigy, A F Sally; Rahman, Azza M.O. Abdel; Shady, A E Eman Abou; Attia, Hazem Ali; Gomaa, Howayda E

doi:10.3923/jms.2006.793.799

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…Other mixed infection types were MP with RSV and INFB with PIVs. Sally, et al found that MP often participated in mixed infections, and, according to one of their hypotheses, patients infected with MP may be susceptible to other infectious pathogens [10]. However, neither the present study nor previous findings could explain these results, so further research is needed to elucidate the question.…”

Section: Resultscontrasting

confidence: 62%

“…Recently, PCR has been reported as a rapid method with high sensitivity that may exceed that of culture, but PCR assays need specialized equipment and the reagents are expensive [9]. Much research shows that the Pneumoslide IgM test is a reasonably sensitive, highly specific, easy, rapid and cost-effective technique for detection of viral or atypical bacterial pathogens [10]. As an indirect immunofluorescense technique for IgM detection, Pneumoslide IgM can simultaneously diagnose 9 pathogens of infectious disease of the respiratory tract, including the Legionella pneumophila serogroup 1 (LP1), Mycoplasma pneumoniae (MP), Coxiella burnetii (COX), Chlamydophila pneumoniae (CP), Adenovirus (ADV), Respiratory syncytial virus (RSV), Influenza A (INFA), Influenza B (INFB), Parainfluenza 1, 2 and 3 (PIVs).…”

Section: Introductionmentioning

confidence: 99%

“…Pneumoslide IgM could detect virus in 25% of patients, whereas viral culture detected only in 16.7%. Sally compared the technique with PCR, and reported the sensitivity and specificity of Pneumoslide IgM for RSV was 75% and 98.1%, respectively, whereas they were 78% and 95% for M. pneumoniae, respectively [10]. So Pneumoslide IgM had reasonable sensitivity and specificity for detection pathogens causing CAP.…”

Section: Introductionmentioning

confidence: 99%

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The aetiology of community associated pneumonia in children in Nanjing, China and aetiological patterns associated with age and season

Chen

Jia

et al. 2015

BMC Public Health

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BackgroundViral and atypical bacterial pathogens play an important role in respiratory tract infection. Using the Pneumoslide IgM test, the presented study explored the aetiology of community-acquired pneumonia and investigated further whether there was an association between age or season and aetiological organisms.MethodsSerum samples, taken between August 2011 and August 2013, from patients with CAP were tested with the Pneumoslide IgM kit. The Pneumoslide IgM technology can simultaneously diagnose 9 viral and atypical bacterial pathogens: Legionella pneumophila serogroup 1 (LP1), Mycoplasma pneumoniae (MP), Coxiella burnetii (COX), Chlamydophila pneumonia (CP), Adenovirus (ADV), Respiratory syncytial virus (RSV), Influenza A (INFA), Influenza B (INFB), Parainfluenza 1, 2 and 3 (PIVs). The data was analyzed by using Statistical Package for the Social Sciences for Windows (SPSS, version 11.0).ResultsOf a total of 1204 serum samples tested, 624 samples were positive. M. pneumoniae was the dominant pathogen, with INFB, PIVs, and RSV ranking second to fourth, respectively. The positive percentages of MP, INFB, PIVs and RSV were found to be associated with age, especially MP, INFB and PIVs. The positive percentages of MP, PIVs and RSV were also found to be associated with season. The positive percentage of MP in autumn was the highest. The positive percentages of LP1 in August and September, ADV in June and INFB in March were relatively higher than that in other months.ConclusionsThe results show there were 4 main viral and atypical bacterial pathogens causing CAP in our study. Some pathogens were found to be associated with age and season. M. pneumoniae was the most predominant pathogen among these 9 pathogens. It is necessary to take preventative measures in order to prevent the spread of these pathogens in susceptible age groups during peak season.

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Section: Resultscontrasting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The aetiology of community associated pneumonia in children in Nanjing, China and aetiological patterns associated with age and season

Chen

Jia

et al. 2015

BMC Public Health

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First molecular evidence of Coxiella burnetii in patients with atypical pneumonia, India

Gangoliya

Kumar

Alam

et al. 2016

Journal of Medical Microbiology

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Prevalence and Correlation of Infectious Agents in Hospitalized Children with Acute Respiratory Tract Infections in Central China

Liu

Xiong

et al. 2015

PLoS ONE

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Acute respiratory tract infections (ARTIs) are associated with significant morbidity and mortality worldwide, especially in children under the age of 5 years. Almost 2 million children die from ARTIs each year, and most of them are from developing countries. The prevalence and correlation of pathogens in ARTIs are poorly understood, but are critical for improving case prevention, treatment, and management. In this study, we investigated the prevalence and correlation of infectious agents in children with ARTIs. A total of 39,756 children with one or more symptoms, including fever, cough, sore throat, tonsillitis, pharyngitis, herpangina, pneumonia, and bronchiolitis, were enrolled in the study. All patients were hospitalized in Wuhan Children’s Hospital between October 1, 2010 and September 30, 2012, and were evaluated for infectious agents. Pathogens, including Mycoplasma pneumoniae, influenza A virus, influenza B virus, adenoviruses, respiratory syncytial virus, parainfluenza virus, Legionella pneumophila, Chlamydophila pneumoniae, and Coxiella burnetii, were screened simultaneously in patient blood samples using anti-pathogen IgM tests. Regression analysis was used to reveal correlations among the pathogens. Our results showed that one or more pathogens were identified in 10,206 patients, and that Mycoplasma pneumoniae, adenoviruses, and influenza B virus were the leading infectious agents. Mixed-infections of pathogens were detected in 2,391 cases, with Mycoplasma pneumoniae as the most frequent pathogen. The most common agents in the co-infections were Mycoplasma pneumoniae and influenza B virus. Regression analysis revealed a linear correlation between the proportion of mixed infections and the incidence of multi-pathogen infections. The prevalence of infectious agents in children with ARTIs was determined. Equations were established to estimate multiple infections by single-pathogen detection. This revealed a linear correlation for pathogens in children with ARTIs. This study provides useful information for improving case prevention and management.

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Pneumoslide-M Technique for Rapid Detection of Atypical Pathogens in Critically ILL Children with Lower Respiratory Tract Infections

Cited by 4 publications

References 26 publications

The aetiology of community associated pneumonia in children in Nanjing, China and aetiological patterns associated with age and season

The aetiology of community associated pneumonia in children in Nanjing, China and aetiological patterns associated with age and season

First molecular evidence of Coxiella burnetii in patients with atypical pneumonia, India

Prevalence and Correlation of Infectious Agents in Hospitalized Children with Acute Respiratory Tract Infections in Central China

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