Pneumonia caused by herpesviruses in recipients of hematopoietic cell transplants

Taplitz, Randy; Jordan, M. Colin

doi:10.1053/srin.2002.33447

Cited by 52 publications

(28 citation statements)

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“…HSV tracheobronchitis was first reported in 1949 [1]. Pulmonary involvement of the lower respiratory tract by HSV in adults has been reported in recipients of solid organ and stem cell transplantations [2,3], and in patients with burns [4], terminal cancer [5,6], AIDS [7] or patients receiving corticosteroids [8]. Herpes tracheobronchitis and bronchopneumonia have also been reported in immunocompetent patients, particularly in the elderly [9,10].…”

Section: Discussionmentioning

confidence: 99%

Endobronchial pseudo-tumour caused by herpes simplex

Upadya

Tilluckdharry²,

Nagy³

et al. 2005

European Respiratory Journal

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Herpes simplex virus (HSV) causes tracheobronchitis and pneumonitis; however, to date, there has only been one report of an endobronchial mass caused by HSV type II.This case study describes a 68-yr-old female with severe kyphoscoliosis who was intubated for acute on chronic hypercapnic respiratory failure and developed blood-tinged endotracheal secretions.Fibreoptic bronchoscopy demonstrated an endobronchial mass in the right middle lobe. Cultures grew HSV type I and biopsy specimens demonstrated cytopathological changes consistent with HSV infection. This is the first reported case of HSV type I presenting as an endobronchial tumour.

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Section: Discussionmentioning

confidence: 99%

Endobronchial pseudo-tumour caused by herpes simplex

Upadya

Tilluckdharry²,

Nagy³

et al. 2005

European Respiratory Journal

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“…However, most of the present diagnostic methods for infectious diseases target a single pathogenic agent. Different pathogenic agents often induce similar clinical symptoms, especially in the early stages of infection (4,8,10,14,17,26,27). Therefore, the appropriate selection of target agents is very important for the rapid and accurate diagnosis and effective treatment of viral infections.…”

Section: Discussionmentioning

confidence: 99%

“…Several anti-viral drugs that are effective for treating herpes virus infections have been developed and are now routinely used in treatment (1,2,18,19,21). However, because different human herpes viruses often elicit similar clinical symptoms, there is an increasing need for the accurate and timely diagnosis of herpes infections in order for these powerful antiviral drugs to be used effectively (5,7,14,15,22,26). Recently, a polymerase chain reaction (PCR) method has been developed that is specific for each virus and serves as a rapid diagnostic method.…”

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confidence: 99%

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Rapid and simultaneous detection of 6 types of human herpes virus (herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpes virus 6A/B, and human herpes virus 7) by multiplex PCR assay

et al. 2009

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A multiplex PCR assay was developed that enabled the simultaneous detection of DNA from 6 types of human herpes virus, HSV-1/2, VZV, EBV, CMV, HHV-6A/B, and HHV-7, using appropriate primer sets and conventional PCR techniques and instruments, with PCR products for each type of virus designed to be easily distinguishable by size. Electropherograms obtained from conventional agarose gels showed that, for each type, the observed number of base pairs corresponded to the intended product and that bands were easily distinguishable from each other. A minimum of 20 copies of viral DNA in a reaction was sufficient to confirm the existence of each of the 6 types of human herpes virus. Comparison of the data obtained from this method and the data obtained from conventional TaqMan PCR using clinical specimens from various sources showed consistent results. The multiplex PCR method reported here for the detection and differentiation of human herpes viruses did not require special equipment or techniques such as hybridization analysis and sequencing analysis and, therefore, enabled us to easily and rapidly detect and identify the 6 types of human herpes virus using conventional methods.

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“…Human herpes viruses (HHVs) have been recognized as clinically important pathogens of pulmonary infections that can result in ALI/ARDS in immunocompromised patients [2,3,4], and they have also been recognized as potential pathogens in nonimmunocompromised critically ill patients [5,6,7,8,9]. However, there have been few comprehensive studies to date on the prevalence and pathogenic role of HHVs in ALI/ARDS patients, partly due to the limited diagnostic tools available for identifying different HHVs in a clinical sample.…”

Section: Introductionmentioning

confidence: 99%

Detection of Herpes Viruses by Multiplex and Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Acute Lung Injury or Acute Respiratory Distress Syndrome

et al. 2013

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Background: Human herpes viruses (HHVs) are important pathogens in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Rapid and efficient diagnostic tools are needed to detect HHVs in the lung in ALI/ARDS patients. Objectives: This study aimed to evaluate the usefulness of multiplex and real-time polymerase chain reaction (PCR) analysis of bronchoalveolar lavage fluid (BALF) for detecting HHV reactivation in ALI/ARDS patients. Methods: Between August 2008 and July 2012, eighty-seven BALF samples were obtained from ALI/ARDS patients with unknown etiology and analyzed for HHVs. The types of HHVs in the BALF samples were determined using qualitative multiplex PCR followed by quantitative real-time PCR. Results: Multiplex PCR identified herpes simplex virus type 1 (HSV-1) (n = 11), Epstein-Barr virus (EBV) (n = 16), cytomegalovirus (CMV) (n = 21), HHV type 6 (HHV-6) (n = 2), and HHV-7 (n = 1) genomic DNA in 35 (40%) of the BALF samples, including 14 (16%) samples containing 2 or 3 HHV types. CMV and EBV reactivation was rare in immunocompetent patients, whereas reactivation of HSV-1 was predominantly observed in intubated patients regardless of their immune status. Overall, HHVs were almost exclusively found in patients with immunosuppression or endotracheal intubation. Real-time PCR detected 0.95-1.59 × 10⁶ copies of viral DNA/μg human genome DNA, and HSV-1 (n = 4), CMV (n = 9), and HHV-6 (n = 1) were identified as potentially pathogenic agents. Conclusions: The implementation of multiplex and real-time PCR of BALF was feasible in ALI/ARDS patients, which allowed efficient detection and quantification of HHV DNA.

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Pneumonia caused by herpesviruses in recipients of hematopoietic cell transplants

Cited by 52 publications

References 0 publications

Endobronchial pseudo-tumour caused by herpes simplex

Endobronchial pseudo-tumour caused by herpes simplex

Rapid and simultaneous detection of 6 types of human herpes virus (herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpes virus 6A/B, and human herpes virus 7) by multiplex PCR assay

Detection of Herpes Viruses by Multiplex and Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Acute Lung Injury or Acute Respiratory Distress Syndrome

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