PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis

Zaid, Mohd Hazani Mat; Abdullah, Jaafar; Yusof, Nor Azah; Sulaiman, Yusran; Wasoh, Helmi; Noh, Mohd Fairulnizal Md; Issa, Rahizan

doi:10.1016/j.snb.2016.10.045

Cited by 92 publications

(20 citation statements)

References 40 publications

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“…The morphology of GP/PANI-modified SPGE surface was studied by FESEM-EDX. It was observed in Figure 3 a,b that the surface structure of GP/PANI-modified electrode turned relatively rough and agglomerate compared to bare SPGE due to the formation of nanoparticle clusters on the electrode surface, which resulted in an increase of active surface area [ 28 , 29 ]. The presence of GP/PANI nanocomposite was also confirmed using EDX analysis.…”

Section: Resultsmentioning

confidence: 99%

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Azmi

Yusof

Kusnin

et al. 2018

Sensors

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A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20–100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.

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Section: Resultsmentioning

confidence: 99%

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Azmi

Yusof

Kusnin

et al. 2018

Sensors

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“…The data in Figure were analyzed using the Z‐view software adopting the Randles‐type circuit (inset of Figure ), which includes the impedance characteristic of the electrodes described by the charge transfer resistance ( R ct ), solution resistance ( R s ) and double layer capacitance ( C dl ). The charge transfer resistance ( R ct ) is a measure of the rate of electron transfer between the redox species and the electrode during the electrochemical reaction course, whereas the solution resistance ( R s ) represents the solution‐phase interference that primarily arises from the electrolyte resistance .…”

Section: Resultsmentioning

confidence: 99%

Green synthesis of conductive polyaniline by Trametes versicolor laccase using a DNA template

Giacobbe

Pezzella

Ventura

et al. 2019

Engineering in Life Sciences

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The green synthesis of highly conductive polyaniline by using two biological macromolecules, i.e laccase as biocatalyst, and DNA as template/dopant, was achieved in this work. Trametes versicolor laccase B (TvB) was found effective in oxidizing both aniline and its less toxic/mutagenic dimer N‐phenyl‐p‐phenylenediamine (DANI) to conductive polyaniline. Reaction conditions for synthesis of conductive polyanilines were set‐up, and structural and electrochemical properties of the two polymers were extensively investigated. When the less toxic aniline dimer was used as substrate, the polymerization reaction was faster and gave less‐branched polymer. DNA was proven to work as hard template for both enzymatically synthesized polymers, conferring them a semi‐ordered morphology. Moreover, DNA also acts as dopant leading to polymers with extraordinary conductive properties (∼6 S/cm). It can be envisaged that polymer properties are magnified by the concomitant action of DNA as template and dopant. Herein, the developed combination of laccase and DNA represents a breakthrough in the green synthesis of conductive materials.

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“…To further study probe coverage and surface modification, MB has been used as a redox label due to the affinity of MB towards DNA. Generally, the time used for the immersion of MB was studied in the range of 10 to 120 min [ 50 ]. In this study, the 60 min was chosen as the period for the immersion of MB.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis

Mohamad

Zaid

Abdullah

et al. 2017

Sensors

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This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10−6–10−9 M with the detection limit of 7.853 × 10−7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors.

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PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis

Cited by 92 publications

References 40 publications

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Green synthesis of conductive polyaniline by Trametes versicolor laccase using a DNA template

Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis

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