PNA-Based MicroRNA Detection Methodologies

Cadoni, Enrico; Manicardi, Alex; Madder, Annemieke

doi:10.3390/molecules25061296

Cited by 29 publications

(32 citation statements)

References 116 publications

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“…Employing PNA in cells or tissues is more challenging, as the matrix becomes increasingly complex, more extensively modified PNAs are required to facilitate solubility and cellular uptake while maintaining selectivity. As a result, PNA has been found to have many applications as a research and diagnostic tool both in the lab and in the clinic [7][8][9], while advancement of PNA therapeutics, especially when compared to other nucleic acid derivatives [10,11], has notably lagged behind. To better understand the potential of PNA-based technologies, we will examine selected research and diagnostic applications highlighting the versatility of PNA as well as key limitations that hinder the extension of these technologies to therapeutic applications.…”

Section: Pna Probes For Research and Diagnostic Applicationsmentioning

confidence: 99%

“…With miRNAs identified as increasingly prominent players in regulating gene expression, detection and quantification of these species is critical to deepening our understanding of miRNAs relation to disease. PNA-based fluorescence and electrochemical sensors of miRNAs have seen increasing use in a number of applications as highlighted in a recent review [9]. Early attempts at miRNA detection mirrored those of mRNA, using in situ enzymatic amplification to generate an optical signal [270].…”

Section: Detection Of Micrornasmentioning

confidence: 99%

“…Since its inception, PNA has become an extremely useful research tool and enabling component of many assays and diagnostics [4,[7][8][9]. On the other hand, development of PNA based therapeutics has notably lagged behind other nucleic acid technologies [10,11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications

Brodyagin

Katkevičs

Kotikam

et al. 2021

Beilstein J. Org. Chem.

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Peptide nucleic acid (PNA) is arguably one of the most successful DNA mimics, despite a most dramatic departure from the native structure of DNA. The present review summarizes 30 years of research on PNA’s chemistry, optimization of structure and function, applications as probes and diagnostics, and attempts to develop new PNA therapeutics. The discussion starts with a brief review of PNA’s binding modes and structural features, followed by the most impactful chemical modifications, PNA enabled assays and diagnostics, and discussion of the current state of development of PNA therapeutics. While many modifications have improved on PNA’s binding affinity and specificity, solubility and other biophysical properties, the original PNA is still most frequently used in diagnostic and other in vitro applications. Development of therapeutics and other in vivo applications of PNA has notably lagged behind and is still limited by insufficient bioavailability and difficulties with tissue specific delivery. Relatively high doses are required to overcome poor cellular uptake and endosomal entrapment, which increases the risk of toxicity. These limitations remain unsolved problems waiting for innovative chemistry and biology to unlock the full potential of PNA in biomedical applications.

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Section: Pna Probes For Research and Diagnostic Applicationsmentioning

confidence: 99%

Section: Detection Of Micrornasmentioning

confidence: 99%

See 1 more Smart Citation

Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications

Brodyagin

Katkevičs

Kotikam

et al. 2021

Beilstein J. Org. Chem.

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show abstract

“…Peptide nucleic acids (PNAs) are DNA mimics with neutral polyamide backbone and have gained a lot of attention as alternatives to DNA probes, in particular for short NA sequences such as micro-RNAs (20-22 mers) that are unlikely to be effectively amplified [49]. Here, the polyamide backbone in PNA probes enables the formation of a PNA-RNA duplex with higher binding strength than those of RNA-RNA or DNA-RNA.…”

Section: Recognition Strategiesmentioning

confidence: 99%

Integrating high-performing electrochemical transducers in lateral flow assay

Perju

Wongkaew

2021

Anal Bioanal Chem

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Lateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

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“…These RNAs may be in the form of mRNAs [ 20 ], lncRNAs [ 21 ], miRNAs, and others. An excellent review in this Special Issue, reported by Cadoni et al [ 22 ], describes the various methodologies used in conjunction with PNA to detect miRNAs. Changes in miRNA expression are associated with a variety of diseases and therefore these miRNAs are considered as ideal disease biomarkers.…”

mentioning

confidence: 99%