Pn-AMPs, the hevein-like proteins from Pharbitis nil confers disease resistance against phytopathogenic fungi in tomato, Lycopersicum esculentum

Lee, Ok Sun; Lee, Bo-Young; Park, Nammi; Koo, Jun Mo; Kim, Young Hoe; D, Theertha Prasad; Karigar, Chandrakant S.; Chun, Hyun Jin; Jeong, Byoung Ryong; Kim, Doh Hoon; Nam, Jaesung; Yun, Jae-Gil; Kwak, Sang‐Soo; Cho, Moo Je; Yun, Dae‐Jin

doi:10.1016/s0031-9422(02)00668-4

Cited by 47 publications

(30 citation statements)

References 29 publications

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“…Ac-AMP2, a 30-residue peptide found in Amaranthus caudatus seeds (79,80), has potent antifungal activity and binds specifically to chitin, a polymer of ␤-1,4-Nacetyl-D-glucosamine (81). Mj-AMP2 (82) is a 36-residue, cationic peptide from the seeds of Mirabilis jalapa and hevein is a 43-residue, multivalent, chitin-binding antifungal peptide found in the rubber tree (Hevea brasiliensis) (83)(84)(85). SHL-1 is a 32-residue peptide found in a spider venom (86,87) with three disulfide bonds that form a cystine knot motif, wherein one disulfide bridge crosses between the other two.…”

Section: Discussionmentioning

confidence: 99%

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

Lehrer

Jung

Ruchala

et al. 2009

The Journal of Immunology

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Four of the six human α-defensins (human neutrophil peptides 1–3 and human α-defensin 5; HD5) have a lectin-like ability to bind glycosylated proteins. Using HD5 as a model, we applied surface plasmon resonance techniques to gain insights into this property. HD5 bound natural glycoproteins > neoglycoproteins based on BSA > nonglycosylated BSA ≫ free sugars. The affinity of HD5 for simple sugars covalently bound to BSA was orders of magnitude greater than its affinity for the same sugars in solution. The affinity of HD5 for protein-bound carbohydrates resulted from multivalent interactions which may also involve noncarbohydrate residues of the proteins. HD5 showed concentration-dependent self-association that began at submicromolar concentrations and proceeded to dimer and tetramer formation at concentrations below 5 μM. The (R9A, R28A) and (R13A, R32A) analogs of HD5 showed greatly reduced self-association as well as minimal binding to BSA and to BSA-affixed sugars. From this and other evidence, we conclude that the extensive binding of HD5 to (neo)glycoproteins results from multivalent nonspecific interactions of individual HD5 molecules with carbohydrate and noncarbohydrate moieties of the target molecule and that the primary binding events are magnified and enhanced by subsequent in situ assembly and oligomerization of HD5. Self-association and multivalent binding may play integral roles in the ability of HD5 to protect against infections caused by viruses and other infectious agents.

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Section: Discussionmentioning

confidence: 99%

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

Lehrer

Jung

Ruchala

et al. 2009

The Journal of Immunology

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“…Strikingly, some HEVEIN-like peptides also affect microorganisms without chitin in their cell wall, such as oomycetes and bacteria (de Souza Cândido et al, 2014). Overexpression of HEVEIN-like peptides resulted in increased plant resistance against various fungal and oomycete pathogens (Lee et al, 2003;R Shukurov et al, 2012).…”

Section: Cysteine-rich Peptidesmentioning

confidence: 99%

The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions

et al. 2015

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“…Potatoes expressing lucerne defensin have been shown to be resistant to Verticillium infection in field trials (Gao et al 2000) and hevein-like proteins expressed in tomato conferred resistance to Phytophtora capsici and Fusarium oxysporum (Lee et al 2003). …”

Section: Specific Targets: Crop Diseasesmentioning

confidence: 99%

The potential of genetically enhanced plants to address food insecurity

2004

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Food insecurity is one of the most important social issues faced today, with 840 million individuals enduring chronic hunger and three billion individuals suffering from nutrient deficiencies. Most of these individuals are poverty stricken and live in developing countries. Strategies to address food insecurity must aim to increase agricultural productivity in the developing world in order to tackle poverty, and must provide long-term improvements in crop yields to keep up with demand as the world's population grows. Genetically enhanced plants provide one route to sustainable higher yields, either by increasing the intrinsic yield capability of crop plants or by protecting them from biotic and abiotic constraints. The present paper discusses a range of transgenic approaches that could increase agricultural productivity if applied on a large scale, including the introduction of genes that confer resistance to pests and diseases, or tolerance of harsh environments, and genes that help to lift the intrinsic yield capacity by increasing metabolic flux towards storage carbohydrates, proteins and oils. The paper also explores how the nutritional value of plants can be improved by genetic engineering. Transgenic plants, as a component of integrated strategies to relieve poverty and deliver sustainable agriculture to subsistence farmers in developing countries, could have a significant impact on food security now and in the future.

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Pn-AMPs, the hevein-like proteins from Pharbitis nil confers disease resistance against phytopathogenic fungi in tomato, Lycopersicum esculentum

Cited by 47 publications

References 29 publications

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions

The potential of genetically enhanced plants to address food insecurity

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