PM 7/24 (4) <i>Xylella fastidiosa</i>

doi:10.1111/epp.12575

Cited by 88 publications

(66 citation statements)

References 75 publications

(74 reference statements)

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“…In the present study, we tested the effectiveness of the compound towards a larger number of trees (i.e., a total of 41 trees) located in two olive groves of the "infected" area of Salento that applied the control strategy during a period of 3 and 4 years of application. The investigation aimed at recording the efficacy of the control strategy during the mid-term period in an area heavily affected by the OQDS [18].…”

Section: Discussionmentioning

confidence: 99%

“…Sampling was performed by following the European and Mediterranean Plant Protection Organization recommendations EPPO Standard PM 7/24 (4) [18].…”

Section: Quantitative Real-time Assessment Of Xfp Within Olive Leavesmentioning

confidence: 99%

“…The control approach we tested was adopted by some farmers of the "infected" area, which start to apply it as a routine measure in their olive groves to face the Xfp epidemic. Since the mid and long-term effectiveness of a control strategy is a basic pre-requisite for its wider application in a territory, and the efficacy of the product "has to be assessed under conditions as near as possible to the conditions of practical use of the product" [18], it is important to verify the robustness of the protocol in farms in production. In order to evaluate the practical application of the control strategy in the mid-term period, we thoroughly assessed both the field symptoms and Xfp DNA concentrations on two olive groves of the "infected" area that regularly applied the biocomplex over three and four consecutive years, respectively, taking into account three different cultivars typical of the Salento areas (Leccino, Ogliarola salentina and Cellina di Nardò).…”

Section: Introductionmentioning

confidence: 99%

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Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)

Tatulli¹,

Modesti²,

Pucci³

et al. 2021

Pathogens

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During recent years; Xylella fastidiosa subsp. pauca (Xfp) has spread in Salento causing relevant damage to the olive groves. Measures to contain the spreading of the pathogen include the monitoring of the areas bordering the so-called “infected” zone and the tree eradication in case of positive detection. In order to provide a control strategy aimed to maintain the tree productivity in the infected areas, we further evaluated the in vitro and in planta mid-term effectiveness of a zinc-copper-citric acid biocomplex. The compound showed an in vitro bactericidal activity and inhibited the biofilm formation in representative strains of X. fastidiosa subspecies, including Xfp isolated in Apulia from olive trees. The field mid-term evaluation of the control strategy assessed by quantitative real-time PCR in 41 trees of two olive groves of the “infected” area revealed a low concentration of Xfp over the seasons upon the regular spraying of the biocomplex over 3 or 4 consecutive years. In particular, the bacterial concentration lowered in July and October with respect to March, after six consecutive treatments. The trend was not affected by the cultivar and it was similar either in the Xfp-sensitive cultivars Ogliarola salentina and Cellina di Nardò or in the Xfp-resistant Leccino. Moreover, the scoring of the number of wilted twigs over the seasons confirmed the trend. The efficacy of the treatment in the management of olive groves subjected to a high pathogen pressure is highlighted by the yielded a good oil production

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Section: Discussionmentioning

confidence: 99%

“…Sampling was performed by following the European and Mediterranean Plant Protection Organization recommendations EPPO Standard PM 7/24 (4) [18].…”

Section: Quantitative Real-time Assessment Of Xfp Within Olive Leavesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)

Tatulli¹,

Modesti²,

Pucci³

et al. 2021

Pathogens

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“…For a comprehensive lipidomic analysis, the pools included the overall analytical complexity of our samples [27,28]. OQDS + and OQDS-samples were molecularly assayed via real-time PCR in technical triplicates [3,29] to verify the presence of Xf and thereon defined as Xf+ and Xf-samples (S1 Fig).…”

Section: Study Site and Sampling Proceduresmentioning

confidence: 99%

“…Xylella fastidiosa (Xf) is one of the top 10 plant pathogenic bacteria [1] and is the cause of an environmental emergency within the European Union (EU). Xf has infected a broad host range [2] of plant species; symptoms vary depending on the combination of the host plant and Xf strain [3]. At present, X. fastidiosa subsp.…”

Section: Introductionmentioning

confidence: 99%

Xylella fastidiosa subsp. pauca and olive produced lipids moderate the switch adhesive versus non-adhesive state and viceversa

Scala¹,

Pucci²,

Salustri

et al. 2020

PLoS ONE

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Global trade and climate change are reshaping the distribution map of pandemic pathogens. One major emerging concern is Xylella fastidiosa, a tropical bacterium recently introduced into Europe from America. In last decades, X. fastidiosa was detected in several European countries. X. fastidiosa is an insect vector-transmitted bacterial plant pathogen associated with severe diseases in a wide range of hosts. X. fastidiosa through a tight coordination of the adherent biofilm and the planktonic states, invades the host systemically. The planktonic phase is correlated to low cell density and vessel colonization. Increase in cell density triggers a quorum sensing system based on mixture of cis 2-enoic fatty acidsdiffusible signalling factors (DSF) that promote stickiness and biofilm. The lipidome profile of Olea europaea L. (cv. Ogliarola salentina) samples, collected in groves located in infected zones and uninfected zones was performed. The untargeted analysis of the lipid profiles of Olive Quick Decline Syndrome (OQDS) positive (+) and negative (-) plants showed a clustering of OQDS+ plants apart from OQDS-. The targeted lipids profile of plants OQDS+ and OQDS-identified a shortlist of 10 lipids that increase their amount in OQDS+ and X. fastidiosa positive olive trees. These lipid entities, provided to X. fastidiosa subsp. pauca pure culture, impact on the dual phase, e.g. planktonic $ biofilm. This study provides novel insights on OQDS lipid hallmarks and on molecules that might modulate biofilm phase in X. fastidiosa subsp. pauca.

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Fast and Reliable Electronic Assay of a Xylella fastidiosa Single Bacterium in Infected Plants Sap

et al. 2022

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Pathogens ultra-sensitive detection is vital for early diagnosis and provision of restraining actions and/or treatments. Among plant pathogens, Xylella fastidiosa is among the most threatening as it can infect hundreds of plant species worldwide with consequences on agriculture and the environment. An electrolyte-gated transistor is here demonstrated to detect X. fastidiosa at a limit-of-quantification (LOQ) of 2 ± 1 bacteria in 0.1 mL (20 colony-forming-unit per mL). The assay is carried out with a millimeter-wide gate functionalized with Xylella-capturing antibodies directly in saps recovered from naturally infected plants. The proposed platform is benchmarked against the quantitave polymerase chain reaction (qPCR) gold standard, whose LOQ turns out to be at least one order of magnitude higher. Furthermore, the assay selectivity is proven against the Paraburkholderia phytofirmans bacterium (negative-control experiment). The proposed label-free, fast (30 min), and precise (false-negatives, false-positives below 1%) electronic assay, lays the ground for an ultra-high performing immunometric point-of-care platform potentially enabling large-scale screening of asymptomatic plants.

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PM 7/24 (4) Xylella fastidiosa

Abstract: Specific scope This Standard describes a Diagnostic Protocol for Xylella fastidiosa.1 It should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Specific approval and amendment First approved in 2004–09. Revised in 2016–09, 2018–04 and 2019–05.2

Cited by 88 publications

References 75 publications

Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)

Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)

Xylella fastidiosa subsp. pauca and olive produced lipids moderate the switch adhesive versus non-adhesive state and viceversa

Fast and Reliable Electronic Assay of a Xylella fastidiosa Single Bacterium in Infected Plants Sap

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