Pluronic® mixed micelles as efficient nanocarriers for benzoporphyrin derivatives applied to photodynamic therapy in cancer cells

Pellosi, Diogo Silva; Tessaro, André Luiz; Moret, Francesca; Gaio, Elisa; Reddi, Elena; Caetano, Wilker; Quaglia, Fabiana; Hioka, Noboru

doi:10.1016/j.jphotochem.2015.08.024

Cited by 64 publications

(46 citation statements)

References 42 publications

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“…The mixtures F127/Brij 700 show an opposite behaviour of F127/Brij 78 and F127/ Brij 98 mixtures as the F127 concentration increases (Figure 3). In the F127/Brij 700 mixtures, the hydrodynamic diameter of particles decreases by increasing concentration of F127, as in the systems formed by mixed micelles obtained of the mixture F127/ SDS in the study proposed by Mondal et al 19 Pellosi et al 20 observed in their studies of pluronic mixed micelles that, in mixtures containing F127/ P123 there was an inverse behaviour: the hydrodynamic diameter of particles increases when the concentration of F127 decreases.…”

Section: Gel Characterisationmentioning

confidence: 79%

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

et al. 2017

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E 100 ) were prepared in the proportions 10/90, 30/70, 50/50 and 70/30, respectively. The characterizations were carried out by tube inversion, rheology, particle size, and theoretical cmcs. The griseofulvin solubilization tests were performed at 25 and 37 °C in micellar solutions of 1 wt. % and quantified by UV/Vis. According to the rheological data, the presence of F127 make the F127/Brij 78 70/30 and F127/Brij 98 70/30 mixtures thermoresponsive at concentrations of 30 and 35 wt.%, with transition fluid/gel in the range of 28-30 °C, ideal for topical use. All of the F127 / Brij 700 mixtures showed moderate distribution polydisperse (PDI < 0.4), as well as mixtures of F127 / Brij 98, with the exception of the mixture with ratio 30/70. Mixtures of F127 / Brij 78 had wide polydisperse distribution (PDI > 0.4). The optimal mixing for oral application would be of F127/Brij 78 because it exhibits greater solubilizing capacity (S cp ) of griseofulvin at temperatures of 25 to 37 °C when compared to F127 alone.

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Section: Gel Characterisationmentioning

confidence: 79%

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

et al. 2017

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“…In a previous work, we demonstrated that the thin-film method followed by freeze-drying (with trehalose as cryoprotectant) allows preparation of poloxamer mixed micelles loaded with VP. 7 Results of this previous study demonstrated that the optimal ratio between P123 and F127 was at 2:1 (w:w). This ratio was maintained in micelles explored here.…”

Section: Properties Of Drug-loaded Micellesmentioning

confidence: 90%

“…Thin-film rehydration provided a fluffy lyophilized product, readily reconstituted in water within 20-30 seconds of manual shaking, as already demonstrated in our previous work. 7 The storage stability of the lyophilized formulations ( Figure S1) demonstrated a small decrease in drug content, with a slight size increase (~5-6 nm) after reconstitution of …”

Section: Properties Of Drug-loaded Micellesmentioning

confidence: 99%

“…6 For example, in a very recent paper, we demonstrated that poloxamer mixed micelles enhanced the solubility and photodynamic activity of very hydrophobic benzoporphyrin derivatives. 7 Sorafenib (SRB) is a drug approved for the treatment of advanced inoperable hepatocellular and advanced renal cancers after oral administration (Nexavar ® ). 8,9 Its possible use for systemic treatment of liver fibrosis 10 and hepatocellular carcinoma [11][12][13] has been recently highlighted.…”

Section: Introductionmentioning

confidence: 99%

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Pluronic<sup>®</sup> P123/F127 mixed micelles delivering sorafenib and its combination with verteporfin in cancer cells

Pellosi¹,

Moret²,

Fraix³

et al. 2016

IJN

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Here, we developed Pluronic ® P123/F127 (poloxamer) mixed micelles for the intravenous delivery of the anticancer drug sorafenib (SRB) or its combination with verteporfin (VP), a photosensitizer for photodynamic therapy that should complement well the cytotoxicity profile of the chemotherapeutic. SRB loading inside the core of micelles was governed by the drug:poloxamer weight ratio, while in the case of the SRB–VP combination, a mutual interference between the two drugs occurred and only specific ratios could ensure maximum loading efficiency. Coentrapment of SRB did not alter the photophysical properties of VP, confirming that SRB did not participate in any bimolecular process with the photosensitizer. Fluorescence resonance energy-transfer measurement of micelles in serum protein-containing cell-culture medium demonstrated the excellent stability of the system in physiologically relevant conditions. These results were in line with the results of the release study showing a release rate of both drugs in the presence of proteins slower than in phosphate buffer. SRB release was sustained, while VP remained substantially entrapped in the micelle core. Cytotoxicity studies in MDA-MB231 cells revealed that at 24 hours, SRB-loaded micelles were more active than free SRB only at very low SRB concentrations, while at 24+24 hours a prolonged cytotoxic effect of SRB-loaded micelles was observed, very likely mediated by the block in the S phase of the cell cycle. The combination of SRB with VP under light exposure was less cytotoxic than both the free combination and VP-loaded micelles + SRB-loaded micelles combination. This behavior was clearly explainable in terms of micelle uptake and intracellular localization. Besides the clear advantage of delivering SRB in poloxamer micelles, our results provide a clear example that each photochemotherapeutic combination needs detailed investigations on their particular interaction, and no generalization on enhanced cytotoxic effects should be derived a priori.

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“…poly(amino acids), poly(esters)) is suitable for hydrophobic PS accommodation while the hydrophilic segments (usually PEG) stabilize the micelle interface with the aqueous environment and allow long circulation in the bloodstream. As an example of efficient micelles for PS delivery, we recently reported Pluronic ® mixed micelles loaded with benzoporphyrin derivatives (BPDs) for cancer treatment [110]. Pluronic ® are surfactant molecules containing two PEG segments and one hydrophobic poly(propylene oxide) (PPO) region arranged in a PEG-PPO-PEG triblock structure, and the proper selection of unit/segment lengths allows the modulation of micelle characteristics.…”

Section: Polymeric Micellesmentioning

confidence: 99%

Strategies for optimizing the delivery to tumors of macrocyclic photosensitizers used in photodynamic therapy (PDT)

Moret

Reddi

2017

J. Porphyrins Phthalocyanines

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This review briefly summaries the principles and mechanisms of action of photodynamic therapy (PDT) as concerns its application in the oncological field, highlighting its drawbacks and some of the strategies that have been or are being explored to overcome them. The major aim is to increase the efficiency and selectivity of the photosensitizer (PS) uptake in the cancer cells for optimizing the PDT effects on tumors while sparing normal cells. Some attempts to achieve this are based on the conjugation of the PS to biomolecules (small ligands, peptides) functioning as carriers with the ability to efficiently penetrate cells and/or specifically recognize and bind proteins/receptors overexpressed on the surface of cancer cells. Alternatively, the PS can be entrapped in nanocarriers derived from various types of materials that can target the tumor by exploiting the enhanced permeability and retention (EPR) effects. The use of nanocarriers is particularly attractive because it allows the simultaneous delivery of more than one drug with the possibility of combining PDT with other therapeutic modalities.

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Pluronic® mixed micelles as efficient nanocarriers for benzoporphyrin derivatives applied to photodynamic therapy in cancer cells

Cited by 64 publications

References 42 publications

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

Pluronic<sup>®</sup> P123/F127 mixed micelles delivering sorafenib and its combination with verteporfin in cancer cells

Strategies for optimizing the delivery to tumors of macrocyclic photosensitizers used in photodynamic therapy (PDT)

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Pluronic® mixed micelles as efficient nanocarriers for benzoporphyrin derivatives applied to photodynamic therapy in cancer cells

Cited by 64 publications

References 42 publications

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

Pluronic<sup>&reg;</sup> P123/F127 mixed micelles delivering sorafenib and its combination with verteporfin in cancer cells

Strategies for optimizing the delivery to tumors of macrocyclic photosensitizers used in photodynamic therapy (PDT)

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Pluronic<sup>®</sup> P123/F127 mixed micelles delivering sorafenib and its combination with verteporfin in cancer cells