Pluronic–lysozyme conjugates as anti-adhesive and antibacterial bifunctional polymers for surface coating

Muszanska, Agnieszka K.; Busscher, Henk J.; Herrmann, Andreas; Mei, Henny C. van der; Norde, Willem

doi:10.1016/j.biomaterials.2011.05.016

Cited by 124 publications

(92 citation statements)

References 40 publications

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“…As stated above, PEGylated surfaces are very useful for this [34]. However, to combine it with anti-bacterial properties is also possible when e.g., an anti-bacterial protein, like lysozyme, is covalently incorporated into the film [89]. Alternatively, cell adhesion is desired in the formation of scaffolds for tissue growth [90].…”

Section: Bio-interfacesmentioning

confidence: 99%

Polymer Directed Protein Assemblies

Rijn

2013

Polymers

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Protein aggregation and protein self-assembly is an important occurrence in natural systems, and is in some form or other dictated by biopolymers. Very obvious influences of biopolymers on protein assemblies are, e.g., virus particles. Viruses are a multi-protein assembly of which the morphology is dictated by poly-nucleotides namely RNA or DNA. This "biopolymer" directs the proteins and imposes limitations on the structure like the length or diameter of the particle. Not only do these bionanoparticles use polymer-directed self-assembly, also processes like amyloid formation are in a way a result of directed protein assembly by partial unfolded/misfolded biopolymers namely, polypeptides. The combination of proteins and synthetic polymers, inspired by the natural processes, are therefore regarded as a highly promising area of research. Directed protein assembly is versatile with respect to the possible interactions which brings together the protein and polymer, e.g., electrostatic, v.d. Waals forces or covalent conjugation, and possible combinations are numerous due to the large amounts of different polymers and proteins available. The protein-polymer interacting behavior and overall morphology is envisioned to aid in clarifying protein-protein interactions and are thought to entail some interesting new functions and properties which will ultimately lead to novel bio-hybrid materials.

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Section: Bio-interfacesmentioning

confidence: 99%

Polymer Directed Protein Assemblies

Rijn

2013

Polymers

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“…Pluronic-Lysozyme conjugates are synthesized as described in [23]. The coating is formed by exposing the surface to the solution of unconjugated Pluronic (PEO) and PluronicLysozyme conjugates in varying ratios and allowing them to adhere.…”

Section: Wet Lab Experimentsmentioning

confidence: 99%

“…The field of antibacterial surfaces is an active area of study, and many research groups are proposing innovative solutions [23,10,25,1,15,34]. In this study, we focus on …”

Section: Introductionmentioning

confidence: 99%

Simulating Anti-adhesive and Antibacterial Bifunctional Polymers for Surface Coating using BioScape

Sharma

Compagnoni

Libera

et al. 2013

Proceedings of the International Conference on Bioinformatics, Computational Biology and Biomedical Informatics

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Traditionally biomaterials development consists of designing a surface and testing its properties experimentally. This trial-and-error approach is limited because of the resources and time needed to sample a representative number of configurations in a combinatorially complex scenario. Therefore, computational modeling is of significant importance in identifying best antibacterial materials to prevent and treat implant related biofilm infections.In this paper we focus on bifunctional surface with polymer brushes and Pluronic-Lysozyme conjugates developed by Henk Busscher's group in Groningen, The Netherlands. The bifunctional brushes act as anti-adhesive due to the unmodified polymer brushes and antibacterial, because of the Pluronic-Lysozyme conjugates. They developed and studied three different surfaces with varying proportions of antibacterial and anti-adhesive properties. In order to aid the development of optimal bifunctional surfaces, we build a three dimensional computational model using BioScape, an agentbased modeling and simulation language developed by Compagnoni's group at Stevens. We model two different experimental phases: adhesion and growth. We use the results of experiments on two surfaces as training data, and we validate our model by reproducing the experimental results from the third surface. The resulting model is able to simulate varying configurations of surface coatings both at adhesion and growth phases at a fraction of the time necessary to perform in-vitro experiments.The output of the model not only plots populations over time, but it also produces 3D-rendered videos of bacteriasurface interactions enhancing the visualization of the system's behavior.

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“…Pluronic block copolymers gained recently much attention as materials for biomedical applications. 35 The block copolymers are composed of PEG and PPO blocks arranged in a triblock architecture PEG n -b-PPO m -b-PEG n . Due to their amphiphilic structure, Pluronic block copolymers form micellar structures similar to DBCs.…”

Section: Supramolecular Assemblies Of Dbcsmentioning

confidence: 99%

DNA Block Copolymers: Functional Materials for Nanoscience and Biomedicine

2012

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W e live in a world full of synthetic materials, and the development of new technologies builds on the design and synthesis of new chemical structures, such as polymers. Synthetic macromolecules have changed the world and currently play a major role in all aspects of daily life. Due to their tailorable properties, these materials have fueled the invention of new techniques and goods, from the yogurt cup to the car seat belts. To fulfill the requirements of modern life, polymers and their composites have become increasingly complex. One strategy for altering polymer properties is to combine different polymer segments within one polymer, known as block copolymers. The microphase separation of the individual polymer components and the resulting formation of well defined nanosized domains provide a broad range of new materials with various properties. Block copolymers facilitated the development of innovative concepts in the fields of drug delivery, nanomedicine, organic electronics, and nanoscience.Block copolymers consist exclusively of organic polymers, but researchers are increasingly interested in materials that combine synthetic materials and biomacromolecules. Although many researchers have explored the combination of proteins with organic polymers, far fewer investigations have explored nucleic acid/polymer hybrids, known as DNA block copolymers (DBCs). DNA as a polymer block provides several advantages over other biopolymers. The availability of automated synthesis offers DNA segments with nucleotide precision, which facilitates the fabrication of hybrid materials with monodisperse biopolymer blocks. The directed functionalization of modified single-stranded DNA by WatsonÀCrick base-pairing is another key feature of DNA block copolymers. Furthermore, the appropriate selection of DNA sequence and organic polymer gives control over the material properties and their self-assembly into supramolecular structures. The introduction of a hydrophobic polymer into DBCs in aqueous solution leads to amphiphilic micellar structures with a hydrophobic polymer core and a DNA corona.In this Account, we discuss selected examples of recent developments in the synthesis, structure manipulation and applications of DBCs. We present achievements in synthesis of DBCs and their amplification based on molecular biology techniques. We also focus on concepts involving supramolecular assemblies and the change of morphological properties by mild stimuli. Finally, we discuss future applications of DBCs. DBC micelles have served as drug-delivery vehicles, as scaffolds for chemical reactions, and as templates for the self-assembly of virus capsids. In nanoelectronics, DNA polymer hybrids can facilitate size selection and directed deposition of single-walled carbon nanotubes in field effect transistor (FET) devices.

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Pluronic–lysozyme conjugates as anti-adhesive and antibacterial bifunctional polymers for surface coating

Cited by 124 publications

References 40 publications

Polymer Directed Protein Assemblies

Polymer Directed Protein Assemblies

Simulating Anti-adhesive and Antibacterial Bifunctional Polymers for Surface Coating using BioScape

DNA Block Copolymers: Functional Materials for Nanoscience and Biomedicine

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