Pluronic F‐127: An Efficient Delivery Vehicle for 3‐(1'‐hexyloxy)ethyl‐3‐devinylpyropheophorbide‐a (HPPH or Photochlor)

Abstract

To determine the impact of delivery vehicles in photosensitizing efficacy of HPPH, a hydrophobic photosensitizer was dissolved in various formulations: 1% Tween 80/5% dextrose, Pluronic P‐123 and Pluronic F‐127 in 0.5%, 1% and 2% phosphate buffer solutions (PBS). HPPH was also conjugated to Pluronic F‐127, and the resulting conjugate (PL‐20) was formulated in PBS. Among the different delivery vehicles, only Pluronic P‐123 displayed significant vehicle cytotoxicity, whereas Pluronic F127 was nontoxic. Compared to PL‐20, HPPH formulated in Tween80 and Pluronic F‐127 showed higher cell‐uptake, but lower long‐term retention in Colon26 cell compared to PL‐20. The higher retention of PL‐20 was similarly observed during in vivo uptake with BALB/c mice baring Ct26 tumors. In contrast to the in vitro uptake experiments, PL‐20 showed slightly higher uptake compared to HPPH formulated in Tween or Pluronic‐F127. A significant difference in pharmacokinetic profile was also observed between the HPPH‐Pluronic formulation and PL‐20. Under similar in vivo treatment parameters (drug dose 0.47 µmol kg−1, light dose: 135 J cm−2 at 24 h post‐injection of PS), HPPH formulated either in Tween or Pluronic F‐127 formulation showed similar in vivo PDT efficacy (20–30% tumor cure on day 60), whereas PL‐20 showed 40% tumor cure (day 60).

Self-assembling synthetic nanoadjuvant scaffolds cross-link B cell receptors and represent new platform technology for therapeutic antibody production

“…S1C) determined using dynamic light scattering was 30.4 nm (±2.7 nm), and the zeta potential was +5.82 mV (±0.89 mV) with a critical micellar concentration (CMC) of about 0.3 μg/ml. In contrast, the mean diameter of Pluronic F127 micelles was 27.1 (±3.2 nm) and the zeta potential was −0.30 mV (±0.17 mV), consistent with literature values ( 24 , 25 ).…”

Photodynamic Therapy in Combination with Doxorubicin Is Superior to Monotherapy for the Treatment of Lung Cancer

“…(h) Impact of Pluronic F-127 formulation in photophysical properties of PS 1: Similar to most of the tetrapyrrole-based PS, e.g., HPPH 30 , the PS 1 derived from chlorophyll-a, on formulating in Pluronic F-127/PBS solution forms aggregation, which significantly reduces its absorption and fluorescence intensities. However, in the presence of HSA (human serum albumin) or BSA (bovine serum albumin), the PS self-aggregation disaggregates, and exhibit photophysical properties similar to the respective monomers observed in organic solvents (e.g., methanol or tetrahydrofuran).…”

“…This report presents (a) the utility of a corresponding non-labeled iodinated PS 1 for treating lung cancer with and without chemotherapy (doxorubicin) in a variety of lung tumors xenografts derived from lung cancer patients and (b) the toxicity and toxicokinetic profiles of the PS formulated in Pluronic F-127 at variable doses in male and female rats and dogs. We and others have previously shown the improved PDT efficacy of certain tetrapyrrolic photosensitizers in a Pluronic-based formulation either by encapsulation or by conjugating the PS with Pluronic F-127 with and without the combination of co-delivery of doxorubicin for overcoming drug resistance in cancer [29][30][31].…”

Curcumin‐loaded Pluronic® F‐127 Micelles as a Drug Delivery System for Curcumin‐mediated Photodynamic Therapy for Oral Application

“…In addition, Pluronics â are characterized by their amphiphilic structure (molecules that have both hydrophilic and hydrophobic regions) and they are able to selfaggregate in aqueous solution (54,55). Several studies have reported Pluronics â as an efficient drug delivery system for cancer treatment and other applications (56)(57)(58)(59)(60).…”